High-Temperature Optoelectronic Transport Behavior of n-TiO Nanoball-Stick/p-Lightly Boron-Doped Diamond Heterojunction.

The n-TiO nanoballs-sticks (TiO NBSs) were successfully deposited on p-lightly boron-doped diamond (LBDD) substrates by the hydrothermal method. The temperature-dependent optoelectronic properties and carrier transport behavior of the n-TiO NBS/p-LBDD heterojunction were investigated. The photoluminescence (PL) of the heterojunction detected four distinct emission peaks at 402 nm, 410 nm, 429 nm, and 456 nm that have the potential to be applied in white-green light-emitting devices.

Mitochondrial segmentation and function prediction in live-cell images with deep learning.

Mitochondrial morphology and function are intrinsically linked, indicating the opportunity to predict functions by analyzing morphological features in live-cell imaging. Herein, we introduce MoDL, a deep learning algorithm for mitochondrial image segmentation and function prediction. Trained on a dataset of 20,000 manually labeled mitochondria from super-resolution (SR) images, MoDL achieves superior segmentation accuracy, enabling comprehensive morphological analysis.

Reevaluating Anti-Inflammatory Therapy: Targeting Senescence to Balance Anti-Cancer Efficacy and Vascular Disease.

Modulating immune function is a critical strategy in cancer and atherosclerosis treatments. For cancer, boosting or maintaining the immune system is crucial to prevent tumor growth. However, in vascular disease, mitigating immune responses can decrease inflammation and slow atherosclerosis progression.

Boosting cation mobility in sol-gel derived organic-inorganic hybrid solid electrolytes through physical conditioning.

Organic-inorganic hybrid materials are explored for application as solid electrolytes for lithium-ion batteries. The material consists of a porous silica network, of which the pores are infiltrated by poly(ethylene oxide) and lithium perchlorate. The synthesis involves two steps: First, the inorganic backbone is created by the acid-catalyzed sol-gel synthesis of tetraethyl orthosilicate to ensure continuity of the backbone in three dimensions.

A generalist medical language model for disease diagnosis assistance.

The delivery of accurate diagnoses is crucial in healthcare and represents the gateway to appropriate and timely treatment. Although recent large language models (LLMs) have demonstrated impressive capabilities in few-shot or zero-shot learning, their effectiveness in clinical diagnosis remains unproven. Here we present MedFound, a generalist medical language model with 176 billion parameters, pre-trained on a large-scale corpus derived from diverse medical text and real-world clinical records.

Investigation on Aluminum Alloy Reflector Mirror Processing Technology Combining Ultrasonic Rolling and Single-Point Diamond Turning.

In the process of aluminum alloy reflector mirror processing, the structural defects of aluminum alloys present bottlenecks restricting the development of aluminum alloy reflector mirror processing technologies. Therefore, this study proposes an aluminum alloy reflector mirror processing method involving ultrasonic rolling and single-point diamond turning. The core idea of this method is to use ultrasonic rolling to pretreat the surface of the workpiece to refine the grains and increase the hardness, then perform single-point diamond turning to improve the optical reflection performance.

Canadian forests are more conducive to high-severity fires in recent decades.

Canada has experienced more-intense and longer fire seasons with more-frequent uncontrollable wildfires over the past decades. However, the effect of these changes remains unknown. This study identifies driving forces of burn severity and estimates its spatiotemporal variations in Canadian forests.

MALT1 promotes the antibacterial immune response by activating NF-κB signaling and enhancing hemocyte phagocytosis in the Chinese mitten crab.

Mucosa-associated lymphoid tissue lymphoma translocation protein 1 (MALT1), a scaffold protein, plays a pivotal role in the NF-κB pathway downstream of T-cell receptors (TCRs) and B-cell receptors (BCRs). As a key signaling hub, MALT1 integrates various pathways, making it essential for both innate and adaptive immunity. However, its role in the antibacterial immune responses of crustaceans remains unclear.

Caspase-8 promotes innate immunity in the Chinese mitten crab by regulating the expression of antimicrobial peptides and apoptosis in hemocyte.

In mammals, caspase-8 primarily functions as an initiator caspase that regulates apoptosis, while in Drosophila, the caspase-8 ortholog DREDD not only induces apoptosis during development but also regulates antimicrobial peptides (AMPs) expression during Gram-negative bacterial infection-induced immune responses. However, the immune-related function of caspase-8 in the crustacean remains unknown. In the present study, the open reading frame of EsCaspase-8 was cloned from the Chinese mitten crab (Eriocheir sinensis).

A multicenter retrospective study of the combination of immune checkpoint inhibitors and chemotherapy regimens with or without liver metastasis for the first-line treatment of advanced gastric cancer.

Background: Several studies have indicated that the use of immune checkpoint inhibitors (ICI) can prolong the survival of patients with advanced gastric cancer (AGC). However, it remains unclear whether the presence of liver metastasis leads to systemic immune suppression, resulting in poorer immune therapy outcomes. This study aims to investigate whether liver metastasis affects the efficacy of ICI in first-line treatment for AGC patients.

Artificial Intelligence-Empowered Multistep Integrated Radiation Therapy Workflow for Nasopharyngeal Carcinoma.

Purpose: To establish an artificial intelligence (AI)-empowered multistep integrated (MSI) radiation therapy (RT) workflow for patients with nasopharyngeal carcinoma (NPC) and evaluate its feasibility and clinical performance.

Methods And Materials: Patients with NPC scheduled for MSI RT workflow were prospectively enrolled. This workflow integrates RT procedures from computed tomography (CT) scan to beam delivery, all performed with the patient on the treatment couch.

Development of differential diagnostic models for distinguishing between limb-girdle muscular dystrophy and idiopathic inflammatory myopathy.

Objective: Limb-girdle muscular dystrophy (LGMD) is usually confused with idiopathic inflammatory myopathy (IIM) in clinical practice. Our study aimed to establish convenient and reliable diagnostic models for distinguishing between LGMD and IIM.

Methods: A total of 71 IIM patients, 24 LGMDR2 patients and 22 LGMDR1 patients diagnosed at our neuromuscular center were enrolled.

Methane emission fluxes and associated microbial community characteristics in a temperate seagrass meadow.

Seagrass meadows are acknowledged as blue carbon ecosystems, yet they are also ideal habitats for methane (CH) release, offsetting their ability to mitigate climate change. The global CH fluxes in seagrass meadows remain highly uncertain due to regional and species biases, and the microbial mechanisms driving methane release are poorly understood. Here, we investigated CH air-sea fluxes, sediment CH emission potential and microbes involved in CH release using geochemical techniques combined with qPCR and Illumina sequencing in a temperate Zostera japonica and Zostera marina mixed meadow.

Intron retention caused by a canonical splicing variant in SSR4-related congenital disorder of glycosylation.

Congenital disorder of glycosylation type Iy (CDG-Iy) is an X-linked monogenic inherited disease caused by variants in the SSR4 gene. To date, a total of 11 variants have been identified in 14 CDG-Iy patients. Our study identified a novel canonical splicing variant, c.

How leisure involvement impacts visitors' perceived health benefits in urban forest parks: examining the moderating role of place attachment.

Urban forest park leisure is a significant means for residents to achieve health and well-being, thus possessing high public health value. However, the relationship between visitors' leisure involvement and perceived health benefits has not been clarified. This study introduced restorative experiences and positive emotions as mediators and place attachment as a psychological moderator, innovatively constructing a stimulus-organism-response-moderator (SORM) integrated model.

ATP-dependent thermoring basis for the heat unfolding of the first nucleotide-binding domain isolated from human CFTR.

Traditionally, the thermostability of a protein is defined by a melting temperature, at which half of the protein is unfolded. However, this definition cannot indicate the structural origin of a heat-induced unfolding pathway. Here, the thermoring structures were studied on the ATP-dependent heat-induced unfolding of the first nucleotide-binding domain from the human cystic fibrosis transmembrane conductance regulator.

Trikafta rescues F508del-CFTR by tightening specific phosphorylation-dependent interdomain interactions.

Unlabelled: Trikafta is well-known for correcting the thermal and gating defects caused by the most common cystic fibrosis mutation F508del in the human cystic fibrosis transmembrane conductance regulator even at physiological temperature. However, the exact pathway is still unclear. Here, the noncovalent interactions among two transmembrane domains (TMD 1 and TMD2), the regulatory (R) domain and two nucleotide binding domains (NBD1 and NBD2), along with the thermoring structures of NBD1, were analyzed around the active gating center.

An In Vitro RNA Editing-Based Reporter Assay for Transcriptional Activity of Therapeutic Gene in Gene Therapy Products.

The expression of therapeutic genes is critical for the efficacy of gene therapy products. However, existing methods such as immunological analysis at the protein level or reverse-transcription PCR at the RNA level are unable to accurately quantify the expression activity of the target gene. Herein, an in vitro RNA editing-based reporter assay was developed to detect specific mRNA.

Integrated single-cell transcriptome and TCR profiles of hepatocellular carcinoma highlight the convergence on interferon signaling during immunotherapy.

Background: Despite the success of immune checkpoint inhibitor (ICI)-based combination therapies in hepatocellular carcinoma (HCC), its effectiveness remains confined to a subset of patients. The development of reliable, predictive markers is important for accurate patient stratification and further mechanistic understanding of therapy response.

Methods: We comprehensively analyzed paired single-cell RNA transcriptome and T-cell repertoire profiles from 14 HCC ascites samples, collected from 7 patients before and after treatment with the combination of sintilimab (anti-PD-1) and bevacizumab (anti-VEGF).

Mapping of the T Cell Landscape of Biliary Tract Cancer Unravels Anatomical Subtype-Specific Heterogeneity.

Biliary tract cancer (BTC), encompassing diseases such as intrahepatic (ICC), extrahepatic cholangiocarcinoma (ECC), and gallbladder cancer (GBC), is not only on the rise but also poses a significant and urgent health threat due to its high malignancy. Genomic differences point to the possibility that these subtypes represent distinct diseases. Elucidation of the specific distribution of T cell subsets, critical to cancer immunity, across these diseases could provide better insights into the unique biology of BTC subtypes and help identify potential precision medicine strategies.

A comparison between single and fractionated doses of albumin-bound paclitaxel in the treatment of advanced esophageal cancer: A multicenter case-control study.

Purpose: At present, albumin-bound paclitaxel combined with platinum is the standard first-line treatment option for advanced esophageal cancer (EC). However, following a hospitalization surge, clinicians may prefer to use albumin-bound paclitaxel as a single dose. The present study aimed to investigate the survival of patients with advanced EC when treated with single or fractionated doses of albumin-bound paclitaxel.

Expanding the spectrum of HSPB8-related myopathy: a novel mutation causing atypical pediatric-onset axial and limb-girdle involvement with autophagy abnormalities and molecular dynamics studies.

Variants in HSPB8 are predominantly associated with peripheral neuropathies, but their occurrence in myopathies remains exceedingly rare. The genetic and clinical spectrum of HSPB8-related myopathy is not yet complete. Herein, we not only described the first Chinese case of HSPB8-related myopathy characterized by a novel heterozygous frameshift variant (c.