Dynamic changes of gut microbiota between the first and second trimester for women with gestational diabetes mellitus and their correlations with BMI: a nested cohort study in China.

Introduction: Gut microbiota (GM) has been implicated in gestational diabetes mellitus (GDM), yet longitudinal changes across trimesters remain insufficiently explored.

Methods: This nested cohort study aimed to investigate GM alterations before 24 weeks of gestation and their association with GDM. Ninety-three Chinese participants provided fecal samples during the first and second trimesters.

Antiviral signaling of a type III CRISPR-associated deaminase.

Prokaryotes have evolved diverse defense strategies against viral infection, such as foreign nucleic acid degradation by CRISPR-Cas systems and DNA/RNA synthesis inhibition via nucleotide pool depletion. Here, we report an antiviral mechanism of type III CRISPR-Cas-regulated ATP depletion, where ATP is converted into ITP by CRISPR-Cas-associated adenosine deaminase (CAAD) upon activation by either cA or cA, followed by hydrolysis into IMP by Nudix hydrolase, ultimately resulting in cell growth arrest. The cryo-electron microscopy structures of CAAD in its apo and activated forms, together with biochemical evidence, revealed how cA/cA binds to the CARF domain and abrogates CAAD autoinhibition, inducing substantial conformational changes that reshape the structure of CAAD and induce its deaminase activity.

Influence of climate and landscape structure on soil erosion in China's Loess Plateau: Key factor identification and spatiotemporal variability.

Climate and landscape structure are widely recognized as the primary drivers of soil erosion; however, the spatiotemporal variability of their effects remains insufficiently understood, limiting our comprehension of the dynamic processes of soil erosion. To address this gap, this study analyzed soil erosion trends on the Loess Plateau from 2000 to 2018. extreme Gradient Boosting was used to identify key climatic and landscape structural factors, while a geographically and temporally weighted regression model was applied to assess the spatiotemporal variability of these influences.

Dual-channel minimally invasive percutaneous cerclage for femoral shaft and metaphysis fractures during the internal fixation with intramedullary nails: case reports and technical description.

When treating femoral fractures with closed reduction intramedullary nail fixation, excessive residual displacement of the fracture end can affect the fixation effect and prolong the healing time, which is difficult to manage intraoperatively. This article introduces a simple steel wire cerclage fixation technique, which is percutaneous and can control the incision within 1 cm without excessive reliance on specially designed surgical instruments. It is suitable for the reduction and fixation of oblique femoral metaphysis fractures and butterfly-shaped femoral shaft fracture blocks.

Efficient manganese ammonia oxidation (Mnammox) and its influencing factors at low temperature: Metal oxide-mediated denitrification process in water bodies.

This study explores the metal oxide-mediated NH-N reduction process: manganese ammonia oxidation efficiency, influencing factors and its resistance to low-temperature environments in water bodies. After 177d of stabilized startup of an up-flow reactor, NH-N removal efficiency was 63.51 %, total nitrogen (TN) removal rate was 0.

Characterization of a Novel Monoclonal Antibody with High Affinity and Specificity against Aflatoxins: A Discovery from Rosetta Antibody-Ligand Computational Simulation.

Aflatoxin B1 (AFB) accumulates in crops, where it poses a threat to human health. To detect AFB, anti-AFB monoclonal antibodies have been developed and are widely used. While the sensitivity and specificity of these antibodies have been extensively studied, information regarding the atomic-level docking of AFB (and its derivatives) with these antibodies is limited.

Knockdown of CPSF4 Inhibits Bladder Cancer Cell Growth by Upregulating NRF1.

Increasing studies have shown that nuclear respiratory factor 1 (NRF1) deficiency frequently occurs in many human diseases, and its activation can protect neurons and other cells from degenerative diseases and malignant tumors. However, how NRF1 is regulated in bladder cancer remains unknown. Our research aims to reveal the role of leavage and polyadenylation-specific factor 4 (CPSF4) on the growth inhibition effect of bladder cancer and clarify its relationship with NRF1.

Assessment of PD-L1 Expression in Non-Small Cell Lung Cancers Using [Ga]Ga-DOTA-WL12 PET/CT.

Assessing programmed death ligand-1 (PD-L1) expression in non-small cell lung cancer (NSCLC), particularly in metastatic cases, remains challenging. In this study, surface plasmon resonance (SPR) analysis and [Ga]Ga-DOTA-WL12 micro-PET/CT imaging are performed. [Ga]Ga-DOTA-WL12 PET/CT and [F]FDG PET/CT are performed on a cohort of 20 patients with NSCLC.

Novel aptasensor based on polyaniline functionalized carboxylated dobby carbon nanotubes and molybdenum disulfide for endotoxin detection.

Endotoxins, also known as lipopolysaccharides (LPS), are present within the cell walls of Gram-negative bacteria and are released upon cellular death, which can pose a significant risk to human and animal health. Due to the minimal amount of endotoxin required to trigger an inflammatory response in human body, the demand for sensitive methods with low endotoxin detection limits is essential necessary. This paper presents a straightforward aptamer sensor which can enhance the conductivity and specific surface area of molybdenum disulfide (MoS) by incorporating carboxylated multi-walled carbon nanotubes (MWCNTs-COOH) and polyaniline (PANI).

AuNi bimetallic aerogel with ultra-high stability applied in smart and portable biosensing.

Glucose detection is of significant importance in providing information to the human health management. However, conventional enzymatic glucose sensors suffer from a limited long-term stability due to the losing activity of the enzymes. In this work, the AuNi bimetallic aerogel with a well-defined nanowire network is synthesized and applied as the sensing nanomaterial in the non-enzymatic glucose detection.

Mining co-location patterns of manufacturing firms using Q statistic and additive color mixing.

The agglomeration effect significantly influences firms' site selection. Manufacturing firms often exhibit intricate spatial co-location patterns that are indicative of agglomerations due to their reliance on material input and product output across various subdivisions of manufacture. In this study, we present an analytical approach employing the Q statistic and additive color mixing visualization to assess co-location patterns of manufacturing firms.

Small Extracellular Vesicles Derived from Altered Peptide Ligand-Loaded Dendritic Cell Act as A Therapeutic Vaccine for Spinal Cord Injury Through Eliciting CD4 T cell-Mediated Neuroprotective Immunity.

The balance among different CD4 T cell subsets is crucial for repairing the injured spinal cord. Dendritic cell (DC)-derived small extracellular vesicles (DsEVs) effectively activate T-cell immunity. Altered peptide ligands (APLs), derived from myelin basic protein (MBP), have been shown to affect CD4 T cell subsets and reduce neuroinflammation levels.

Portable visual and electrochemical detection of hydrogen peroxide release from living cells based on dual-functional Pt-Ni hydrogels.

It is important to monitor the intra-/extracellular concentration of hydrogen peroxide (HO) in biological processes. However, miniaturized devices that enable portable and accurate HO measurement are still in their infancy because of the difficulty of developing facile sensing strategies and highly integrated sensing devices. In this work, portable HO sensors based on Pt-Ni hydrogels with excellent peroxidase-like and electrocatalytic activities are demonstrated.

Recent design strategies and applications of organic fluorescent probes for food freshness detection.

Food spoilage poses a significant risk to human health, making the assessment of food freshness essential for ensuring food safety and quality. In recent years, there has been rapid progress in the development of fast detection technologies for food freshness. Among them, organic fluorescent probes have garnered significant attention in the field of food safety and sensing due to their easy functionalization, high sensitivity, and user-friendly nature.

Quantitative analysis method of Panax notoginseng based on thermal perturbation terahertz two-dimensional correlation spectroscopy.

This paper proposes a Panax notoginseng (P. notoginseng) quantitative analysis based on terahertz time-domain spectroscopy and two-dimensional correlation spectroscopy (2DCOS). By imposing temperature perturbation combined with 2DCOS, the one-dimensional absorbance spectra were transformed into 2DCOS synchronous spectra, which reflected the differences in characteristic information between different P.

Effect of Heat-Moisture Treatment on the Structure and Digestibility of Sweet Potato Starch.

The objective of this study was to investigate the effect of temperature changes during heat-moisture treatment (HMT) on the appearance, structure and digestibility of sweet potato starch (SPS). The results showed that after HMT, there were depressions, cavities and fragments on the surface of SPS particles. The polarized crosses of SPS were irregular and partially blurred.

Single-cell analysis of spinal cord injury reveals functional heterogeneity of oligodendrocyte lineage cells.

Spinal cord injury (SCI) is a traumatic condition that causes myelin destruction and neuronal death, making it challenging to reverse. In spinal cord tissue, oligodendrocyte progenitor cells and oligodendrocytes are essential for maintaining myelin morphology and axon regeneration. The decrease in oligodendrocyte lineage cells after SCI is a major factor contributing to the difficulty in restoring spinal cord function.

Effects of sodium carboxymethyl cellulose on storage stability and qualities of different frozen dough.

Hydrocolloids as Additives have been used for improving the quality of frozen dough for a long time. In this work, the effects of sodium carboxymethyl cellulose (CMC) on quality changes of frozen dough in storage were studied. The water loss rate of the dough and water holding capacity were measured.

Arsenic Contents, Speciation and Toxicity in Germinated Rice Alleviated by Selenium.

Rice can accumulate more organic and inorganic arsenic (iAs) than other crop plants. In this study, the localization of As in rice grains was investigated using High Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry (HPLC-ICP-MS) based on 26 rice varieties collected from two provinces. In all the samples, the total As contents in polished rice were 0.

PE-STOP: A versatile tool for installing nonsense substitutions amenable for precise reversion.

The rapid advances in genome editing technologies have revolutionized the study of gene functions in cell or animal models. The recent generation of double-stranded DNA cleavage-independent base editors has been suitably adapted for interrogation of protein-coding genes on the basis of introducing premature stop codons or disabling the start codons. However, such versions of stop/start codon-oriented genetic tools still present limitations on their versatility, base-level precision, and target specificity.