Molecular characterization of a MOSPD2 homolog in the barbel steed (Hemibarbus labeo) and its involvement in monocyte/macrophage and neutrophil migration.

Motile sperm domain containing 2 (MOSPD2) is a single-pass membrane protein to which until recently little function had been ascribed. Although its mammalian homologs have been identified, the status of the mospd2 gene in lower vertebrates is still unknown. In the present study, cDNA of the mospd2 gene of barbel steed (Hemibarbus labeo) was cloned and sequenced to characterize its potential involvement in the innate immune system of this fish.

Intratumoral estrogen sulfotransferase induction contributes to the anti-breast cancer effects of the dithiocarbamate derivative TM208.

Aim: Sulfotransferase-catalyzed sulfation is the most important pathway for inactivating estrogens. Thus, activation of estrogen sulfotransferase (EST) may be an alternative approach for the treatment of estrogen-dependent breast cancer. In this study we investigated the involvement of EST in anti-breast cancer effects of the dithiocarbamate derivative TM208 in vitro and in vivo.

Dopamine D1 receptor activation induces dehydroepiandrosterone sulfotransferase (SULT2A1) in HepG2 cells.

Aim: Dopamine receptors are present in the nervous system and also widely distributed in the periphery. The aim of this study was to investigate the role of D1 subtype dopamine receptors (DRD1) in the regulation of dehydroepiandrosterone sulfotransferase (SULT2A1) in HepG2 cells.

Methods: HepG2 cells were treated with DRD1 agonists with or without DRD1 antagonist for 9 d.

[Soil respiration characteristics in the clear-cutting site of Quercus aliena var. acuteserrata forest in Xiaolong Mountain in Qinling Mountains].

By using Li-6400 portable photosynthesis system with soil chamber, the soil respiration rates (Rs) of Quercus aliena var. acuteserrata forest land (control site) and their clear-cutting site were measured in Xiaolong Mountain of Qinling Mountains from May 2011 to April 2012 to understand the diurnal and monthly dynamics of soil respiration rate and the influences from soil temperature, soil moisture, soil physical and chemical properties. The results showed that both diurnal and monthly dynamics of soil respiration rate presented a single-peak curve, similar to the variation of soil temperature at the clear-cutting and control sites.

[Combined treatment with areola approach for capsular contracture after breast augmentation with implants].

Objective: To investigate the combined treatment with areola approach for capsular contracture after breast augmentation with implants.

Methods: From Feb. 2005 to Jun.

[Spiral computed tomography imaging of internal carotid artery diameter: a report of 260 cases].

Objective: To explore the diameter of internal carotid artery by arterial phase imaging of multi-slice spiral computed tomography (CT).

Methods: A total of 260 routine brain CT scans of the examiner were performed. And the arterial phase images of carotid artery were acquired, observed and measured through a three-dimensional reconstruction workstation.

[The telomerase activity of human adipose derived stem cells during proliferation and differentiation in vitro].

Objective: To investigate the telomerase activity of human adipose derived stem cells (ADSCs) during proliferation and differentiation in vitro.

Methods: ADSCs were highly purified and cultured in vitro. The morphology, phenotype and biological properties of the cultured ADSCs were observed by flow cytometer.

Significance of MMP-2 and TIMP-2 mRNA expressions on glomerular cells in the development of glomerulosclerosis.

Objective: To study the expressions of MMP-2 and TIMP-2 mRNA on cultured rat mesangial cells (MsC) and in human diseased glomeruli, and to explore their significance in the development of glomerulosclerosis.

Methods: The expressions of MMP-2, TIMP-2, and Col IV mRNA on cultured rat MsC stimulated by IL-1 or/and TGF-beta1 were investigated through Northern blot analysis. The levels of MMP-2 and TIMP-2 mRNA expressions and immunoreactivity of PCNA and Col IV in human diseased glomeruli from renal biopsies of lupus nephritis (LN) patients were examined by in situ hybridization and immunohistochemistry, respectively.