Development of ELISAs for the detection of urogenital chlamydia trachomatis infection targeting the pORF5 protein.

Objective: To prepare antibodies against pORF5 plasmid protein of Chlamydia trachomatis and develop double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISAs) for the detection of genital C. trachomatis infections.

Methods: The pORF5 protein was expressed in Escherichia coli and used to immunize BALB/c mice and New Zealand rabbits to produce monoclonal antibodies (mAbs) and polyclonal antibody (pAb) for DAS-ELISAs.

Localization and characterization of the hypothetical protein CT440 in Chlamydia trachomatis-infected cells.

The inclusion membrane proteins play potentially important roles in chlamydial biology and pathogenesis. Here we localized and characterized the hypothetical protein CT440 in Chlamydia trachomatis-infected cells. The open reading frame (ORF) encoding the CT440 protein from the C.

[Cloning and cellular localization of pORF8 plasmid protein of Chlamydia trachomatis].

Objective: To clone the plasmid protein pORF8 of Chlamydia trachomatis and localize its expression in Chlamydia-infected cells.

Methods: pORF8 gene was amplified and cloned into pGEX-6p vector, and the pORF8 fusion protein was expressed in E.coli XL1 Blue.

[Preparation and identification of monoclonal antibodies against Chlamydia trachomatis Tarp protein].

Objective: To obtain monoclonal antibodies (mAbs) against Chlamydia trachomatis Tarp protein.

Methods: Chlamydia trachomatis serovar D recombinant Tarp fusion protein was cloned and expressed. Balb/c mice were immunized with recombinant Tarp fusion protein, and the spleen cells of the immunized mice were fused with SP2/0 mouse myeloma cells.

[Early production of interleukin-17 in airway upon Chlamydia trachomatis infection increases the local secretion of IL-6 and MIP-2].

Objective: To evaluate the early interleukin-17 (IL-17) production in airway upon Chlamydia trachomatis infection and its relationship with the secretion of interleukin-6 (IL-6) and macrophage inflammatory protein 2 (MIP-2) in local site.

Methods: In vivo, a murine model of pneumonia induced by intranasal inoculation with Chlamydia trachomatis mouse pneumonitis (MoPn, now classified as a new species C. muridarum) was used for the study.

[Cloning and expression of Chlamydia trachomatis OmcBc gene and antigenicity analysis of the protein].

Objective: To investigate the antigenicity of recombinant Chlamydia trachomatis (Ct) OmcBc protein and search for the new target for early diagnosis of Chlamydia infection and Chlamydia vaccine development.

Methods: The C fragment of OmcB encoding the amino acids from T270 to T553 was amplified from Chlamydia serovar D genomic DNA. The pGEX-6p-Ct OmcBc expression plasmid was constructed and transformed into E.

Three-dimensional reconstruction of the uterine vascular supply through vascular casting and thin slice computed tomography scanning.

It was the objective of this study to construct a model of the uterine vascular supply through vascular casting and thin slice computed tomography scanning. This will provide a teaching aide for the understanding of uterine artery embolization (UAE) procedures, as well as normal uterine and ovarian arterial anatomy. Using 20% chlorinated poly vinyl chloride, we infused and cast a set of a normal uterus, vagina and bilateral adnexa through the uterine artery and ovarian artery.

[Localization of the hypothetical protein CT249 in the Chlamydia trachomatis inclusion membrane].

To characterize the hypothetical protein CT249 using antibodies raised with CT249 fusion protein. The open reading frame (ORF) coding for CT249 in the Chlamydia trachomatis serovar L2 genome was cloned into the pGEX-6p2 vector using the restriction enzymes BamH I and Not I. The recombinant plasmid pGEX-6p2-CT249 was transformed into XL1-blue bacteria and the gene CT249 was expressed as fusion proteins with the glutathione-s-transferase (GST) tagged to the N-terminus.