[Anti-schistosomiasis effect induced by full length DNA vaccine coding paramyosin of Schistosoma japonicum in C57BL/6 mice].

Objective: To observe the anti-schistosomiasis effect in mice immunized with Sjc97 DNA vaccine.

Methods: C57BL/6 mice were vaccinated intramuscularly with the Sjc97 DNA twice at an interval of 3 weeks, and challenged with 30 +/- 2 cercariae of Schistosoma japonicum three weeks after immunization. Mice in blank plasmid vector control and infection control groups were also infected with same number of cercariae.

[Immunological characteristics induced in mice by nucleic acid vaccine harboring the gene encoding GAPDH in Schistosoma japonicum].

Objective: To study the immunological characteristics induced in C57BL/6 mice by nucleic acid vaccine harboring the gene encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of Schistosoma japonicum.

Methods: The gene encoding GAPDH of S. japonicum from screening of cDNA library was amplified using universal primer T3 promoter and T7 promoter, the PCR product was cloned into the T-A vector pT-Adv.

[Screening of cDNA library of Schistosoma japonicum with sera from rabbits vaccinated with ultraviolet-attenuated schistosomula].

Objective: To search new potential schistosomiasis vaccine by screening cDNA library with sera of rabbits vaccinated with attenuated larvae.

Methods: Schistosoma japonicum (Sj) adult worm cDNA library was screened with sera of rabbits vaccinated with ultraviolet-attenuated schistosomula and by sera of infected rabbits, and the inserts of positive clones were amplified and sequenced.

Results: Six kinds of Sj genes were obtained after three rounds of screening.

[Schistosoma japonicum: screening of cDNA library with sera from self-cured water buffaloes (Bos buffelus)].

Objective: To understand and identify the molecules related to the self-cure of Schistosoma japonicum infection in water buffaloes.

Methods: The S. japonicum adult worm cDNA library was immunologically screened with the sera of self-cured water buffaloes.

[Preliminary observation on specific antibody level and reduction of ovulation induced by recombinant Schistosoma japonicum 26 kDa GST antigen in water buffaloes].

Objective: To observe the specific antibody level and reduction of egg-laying induced by a recombinant Schistosoma japonicum 26 kDa GST antigen (rSjc26 GST) in water buffaloes.

Methods: 20 water buffaloes were randomly divided into two groups, the vaccination group and control group, with 10 buffaloes each. The subjects in vaccination group were immunized with rSjc26 GST antigen while the control received adjuvant only.