[Sperm sorting based on the imitation of the physiological process on the microfluidic chip].

Objective: To establish a new method for sperm sorting by imitating the physiological process of sperm-cervical mucus interaction on the microfluidic chip.

Methods: We designed a microfluidic chip to imitate the physiological process of natural sperm sorting in the microchannel based on the interaction between sperm and cervical mucus, and obtained motile sperm after the interaction. Meanwhile, we established an integrated real-time sperm detection reservoir on this chip to determine sperm parameters using the computer-assisted sperm analysis system.

[Effects of a microfluidic sperm sorter on sperm routine parameters and DNA integrity].

Objective: To investigate the effects of a microfluidic sperm sorter on the routine parameters and DNA integrity of human sperm.

Methods: We divided 40 semen samples into two aliquots and performed sperm sorting using a self-made polydimethylsiloxane microfluidic sperm sorter and the swim-up method, respectively. Then we evaluated and compared the effects of these two methods on the sperm routine parameters and DNA integrity by computer-assisted sperm analysis and sperm chromatin dispersion test.