Development and application of an AllGlo probe-based qPCR assay for detecting knockdown resistance (kdr) mutations in Anopheles sinensis.

Background: Anopheles sinensis is one of the most important malaria vectors in China and other Southeast Asian countries. High levels of resistance have been reported in this species due to the long-term use of insecticides, especially pyrethroids, for public health and agricultural purposes. Knockdown resistance (kdr) caused by a single base pair mutation in the gene encoding the sodium channel is strongly associated with pyrethroid insecticide resistance in many Anopheles mosquitoes.

[Epidemic situation and control strategy of imported malaria in Hubei Province from 2006 to 2011].

Objective: To understand the characteristics of imported malaria in Hubei Province so as to provide the evidence for formulating the prevention and control strategy.

Methods: The data of all imported malaria cases from 2006-01 to 2011-07 were retrospectively analyzed, and at the same time, the imported malaria cases since 2010 were interviewed through telephone and the cases since 2011-05 were interviewed face to face. All data were analyzed with EpiInfo.

[Molecular identification of Anopheles hyrcanus complex by using single enzyme digestion PCR-RFLP method].

Objective: To establish a novel molecular identification method for discrimination of members within Anopheles hyrcanus complex.

Methods: The sequences of the ribosomal DNA second internal transcribed spacer (rDNA ITS2) region of An. hyrcanus complex, including An.

[Evaluation on malaria situation in areas along Yellow River and Huaihe River by indirect fluorescent antibody test].

Objective: To evaluate malaria situation by indirect fluorescent antibody test (IFAT) in 12 villages of six counties along Yellow River and Huihe River in Anhui, Henan and Hubei Provinces.

Methods: Cross sectional seroepidemiologic survey was conducted through IFAT in Anopheles sinensis area and A. anthropophagus plus A.

[Study on the thresholds of malaria transmission by Anopheles anthropophagus in Hubei Province].

Objective: To study the thresholds and potential of malaria transmission by Anopheles anthropophagus in Hubei Province and provide indicators for the disease surveillance, early warning, prevention and control in the locality.

Methods: From July to August 2001, field investigations on vectors and malaria situation were carried out in the village of Yanjiafan, Suizhou City, where the malaria incidence was high. The entomological investigations included the man-biting rate, the proportion of parous anophelines, the human blood index and the blood preference.