Publications by authors named "Guang-Ping Ruan"
Article Synopsis
- Aging leads to various degenerative diseases, particularly affecting the skin, which undergoes significant structural and functional changes, such as reduced collagen production and increased wrinkles.
- Mesenchymal stromal cells (MSC), particularly those derived from umbilical cords (UC-MSC), show promise in cell therapy for delaying skin aging due to their regenerative properties.
- The paper aims to explore the mechanisms by which UC-MSCs contribute to skin rejuvenation, providing a foundation for future research in combating skin aging.
Article Synopsis
- Reduced thymic epithelial cells (TECs) are linked to thymic degeneration, but umbilical cord mesenchymal stem cells (UCMSCs) have shown promise in restoring TEC function and structure despite the unclear mechanisms behind this effect.
- The study involved inducing senescence in TECs and then coculturing them with UCMSCs, which resulted in significant changes in gene expression, particularly in the PI3K-AKT signaling pathway.
- The findings suggest that UCMSCs enhance TEC proliferation by upregulating VEGFA and promoting key cell cycle proteins, ultimately reversing age-related dysfunction in TECs.
Mechanisms of Stem Cells and Their Secreted Exosomes in the Treatment of Autoimmune Diseases.
Curr Stem Cell Res Ther
September 2024
Article Synopsis
- - Stem cells are effective in treating autoimmune diseases due to their ability to self-renew and differentiate, primarily by regulating immune responses.
- - Their therapeutic impact involves adjusting the balance of auxiliary cells and inflammatory factors to help reduce inflammation in patients.
- - Stem cell-derived secretory bodies, which have low immunogenicity and can target injury sites, are gaining importance in treatment, especially when combined with other materials to prolong their effectiveness.
Article Synopsis
- Recent studies indicate that umbilical cord mesenchymal stem cells (HA-MSCs) may counteract ovarian aging, although the specific cellular and molecular mechanisms are not yet fully understood.
- A 10X Genomics single-nucleus transcriptome sequencing experiment was conducted on the ovaries of macaque monkeys after HA-MSC treatment to explore these mechanisms.
- Results showed that HA-MSC treatment improved the structure and function of ovaries by enhancing cell proliferation and reversing senescence in aging cells, suggesting a promising new approach for treating ovarian aging.
Article Synopsis
- tRFs and tiRNAs are small noncoding RNA molecules that play crucial roles in both prokaryotic and eukaryotic cells.
- Researchers identified three specific tRF molecules with increased expression in reprogrammed cells and tested them in human umbilical cord mesenchymal stem cells.
- The transfection of these tRFs led to a rise in pluripotent factors like OCT4 and NANOG, enhanced cell activity, reduced apoptosis, and changes in the cell cycle, indicating their potential to promote cell rejuvenation and increase pluripotency.
Background: The tumorigenesis of infused umbilical cord mesenchymal stem cells (UC-MSCs) is being preclinically evaluated.
Methods: We observed tumor formation in NOD SCID mice after a single subcutaneous injection of hUC-MSCs and the effect of these cells on tumor growth in tumor-bearing mice. Three generations (P5, P7, and P10) of hUC-MSCs (1 × 10) from two donors (hUC-MSC1 and hUC-MSC2) were inoculated subcutaneously into NOD SCID mice.
Article Synopsis
- The study investigates the effects of mouse umbilical cord mesenchymal stem cells (mUCMSCs) on the reproductive health of ageing C57 mice, focusing on their potential to repair ovarian function.
- Aging mice with diminished ovarian function received mUCMSC injections, resulting in increased ovarian size, the presence of follicles, and enhanced hormone levels compared to a control group.
- The findings indicate that mUCMSCs can reduce cell apoptosis and oxidative stress in granulosa cells by altering specific gene expressions and activating key cellular pathways, suggesting a potential for regenerative therapies in reproductive health.
Based on the characteristics of modern weapon injury, a repetitive model of traumatic systemic inflammatory response syndrome (SIRS) and an evaluation system were established. The models were treated with GFP-labeled tree shrew umbilical cord mesenchymal stem cells (UCMSCs). Forty out of 50 tree shrews were used to make a unilateral femoral comminuted fracture.
View Article and Find Full Text PDFThe effects of BMMSC treatment on lung tissue degeneration in elderly macaques.
Stem Cell Res Ther
March 2021
Article Synopsis
- Age-related degeneration of lung tissue increases the risk of severe lung diseases, making the search for anti-aging treatments crucial.
- A study on elderly macaque models found that administration of bone marrow-derived mesenchymal stem cells (BMMSCs) significantly improved lung health by reducing fibrosis and inflammation.
- BMMSC treatment was effective in decreasing harmful oxidative stress and enhancing the function and proliferation of lung cells, suggesting it could be a viable method to combat age-related lung degeneration.
Article Synopsis
- Ischemia-reperfusion injury significantly contributes to acute kidney injury and affects recovery after kidney transplants; this study explores the potential protective role of stem cell transplantation in such injuries.
- The experiment involved 40 rabbits divided into four groups, with 30 establishing the injury model, and 10 serving as untreated controls; comparisons were made based on treatments with either induced or non-induced peripheral blood mononuclear cells (PBMCs).
- Results showed that rabbits treated with induced PBMCs exhibited improved renal function and less severe pathological changes, indicating the therapeutic potential of these induced stem cells in mitigating kidney injury.
Article Synopsis
- A mouse model for allergic rhinitis (AR) was developed using BALB/c mice to aid in future research on the condition.
- * The study involved preparing human umbilical cord mesenchymal stem cells (hUCMSCs), which were shown to be effective for tracking in animal studies and possibly for AR treatment.
- * The transplantation of hUCMSCs, particularly through tail vein injection, demonstrated positive effects on the AR model, with a significant presence of these cells in the nasal area after two weeks.
Article Synopsis
- The study aimed to investigate how allogeneic umbilical cord mesenchymal stem cell (mUCMSC) transplantation affects the thymus in aging mice, potentially offering a new treatment for age-related thymic atrophy.
- Results indicated that mUCMSC treatment improved thymus structure and function by analyzing changes in thymic tissue and immune cell markers, as well as promoting hair regeneration in the mice.
- The findings suggest that mUCMSCs can reduce thymus aging by affecting specific senescence-related genes, though further research is needed to fully understand the underlying mechanisms.
Article Synopsis
- Inflammatory bowel disease (IBD) causes chronic inflammation in the gastrointestinal tract, and researchers are exploring new treatments with minimal side effects.
- Mouse models of IBD were created using a solution called DSS, allowing scientists to test the effects of umbilical cord mesenchymal stem cells (UCMSCs) from both mice and humans.
- The study found that UCMSCs improve survival rates and promote healing by enhancing key proteins in the intestines, offering a promising avenue for future IBD therapies.
Article Synopsis
- - The study explored the link between bone marrow mesenchymal stem cells (BMSCs) and aging by establishing an aging macaque model, analyzing how BMSCs from young and aged macaques differ in their characteristics.
- - Researchers found that aging BMSCs showed decreased levels of key genes associated with aging, such as TERT, SIRT1, and SIRT6, using techniques like RT-PCR and Western blot.
- - The team compared the effects of young and aged BMSCs on 293T cells, investigated secreted cytokines, and analyzed transcriptomes of peripheral blood mononuclear cells from macaques to understand gene transcription and regulatory mechanisms related to aging.
Article Synopsis
- Umbilical cord mesenchymal stem cells (UC-MSCs) demonstrate strong potential to improve survival and mitigate damage from acute radiation injury in tree shrews.
- The treatment resulted in significant reductions in lung inflammation, apoptosis, and fibrosis, alongside enhanced blood counts and hematopoiesis.
- Analysis indicated increased levels of anti-inflammatory cytokines and growth factors in treated animals, while inflammatory and fibrosis-related factors decreased, suggesting UC-MSCs could be a promising therapy for radiation injuries.
Article Synopsis
- This study evaluates the safety and immunological effects of umbilical cord mesenchymal stem cells from cynomolgus monkeys (mUC-MSCs) to guide human clinical applications.
- Eighteen cynomolgus monkeys were split into three groups to assess the impact of different dosages, with observations made for any toxic reactions after repeated cell administrations.
- Results showed no deaths or serious health issues, with some minor changes in certain blood levels that normalized after 28 days, suggesting a safe dosage for potential human use is significantly higher than current clinical amounts.
Article Synopsis
- Researchers are exploring a novel cell transplantation method using reprogrammed peripheral blood mononuclear cells (PBMCs) turned into induced mesenchymal stem cells (iMSCs) for treating chronic renal insufficiency.
- An animal model of chronic renal insufficiency, created through unilateral ureteral obstruction, was used to evaluate the effectiveness of these iMSCs compared to non-induced PBMCs.
- The study aims to assess the therapeutic potential of iMSC transplantation in managing chronic renal issues, hoping to provide new treatment options.
Article Synopsis
- * This study explored the use of islet-like cells from umbilical cord mesenchymal stem cells (UC-MSCs) in tree shrews with type 2 diabetes, showing effectiveness in reducing disease severity.
- * UC-MSCs were found to differentiate into functional insulin-secreting cells through stimulation from high glucose and activation of Notch signaling, making this method a potentially simple and cost-effective diabetes treatment.
Background: The establishment of a tree shrew model for systemic lupus erythematosus (SLE) provides a new method to evaluate the pathogenesis of autoimmune diseases.
Methods: Eighty tree shrews were randomly divided into four groups receiving either an intraperitoneal injection of pristane, lipopolysaccharide (LPS), or pristane and LPS, or no injection. Three weeks after injection, the SLE model tree shrews were divided into the model group and the treatment group.
The aim of this study was to establish a tree shrew metabolic syndrome model and demonstrate the utility of MSCs in treating metabolic syndrome. We used tree shrew umbilical cord mesenchymal stem cell (TS-UC-MSC) transplantation for the treatment of metabolic syndrome to demonstrate the clinical application of these stem cells and to provide a theoretical basis and reference methods for this treatment. Tree shrew metabolic syndrome model showed significant insulin resistance, high blood sugar, lipid metabolism disorders, and hypertension, consistent with the diagnostic criteria.
View Article and Find Full Text PDFWe previously found that chicken egg white extract could promote cell survival and proliferation. In the present study, we further separated this extract into its components to identify those primarily responsible for promoting cell proliferation. Components of differing molecular weight were separated from chicken egg white extract by ultrafiltration and 293T cell cultures were supplemented with various concentrations.
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- Embryonic-like stem cells (ELSCs) show potential in muscle regeneration, expressing markers for embryonic stem cells and differentiating into various cell types more effectively than mesenchymal stromal cells (MSCs).
- In a study using dystrophin/utrophin double knock-out mice (a model for Duchenne muscular dystrophy), ELSCs were injected via tail vein and led to improved motor function and reduced creatine kinase levels two months post-transplant.
- Results indicated that ELSCs enhanced dystrophin expression and muscle tissue quality compared to MSCs, supporting their potential for therapeutic use in muscle disorders.
Diabetic nephropathy (DN) is a common microvascular complication of diabetes. We used a new DN model in tree shrews to validate the use of bone-marrow mesenchymal stem cell (BM-MSC) transplantation to treat DN. The DN tree shrew model was established by a high-sugar and high-fat diet and four injections of streptozotocin.
View Article and Find Full Text PDFBone marrow mesenchymal stem cells (BMSCs) are self-renewing, multipotent cells that can migrate to pathological sites and thereby provide a new treatment in diabetic animals. Superparamagnetic iron oxide/4',6-diamidino-2-phenylindole (DAPI) double-labeled BMSCs were transplanted into the pancreatic artery of macaques to treat type 2 diabetes mellitus (T2DM). The treatment efficiency of BMSCs was also evaluated.
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