Age-Related Association Between Unilateral Single-Channel and Double-Channel Surgery and Postoperative Multifidus Muscle Atrophy and Fat Infiltration.

Background: Unilateral biportal endoscopic laminotomy (UBE) and percutaneous endoscopic laminotomy (PEL) are minimally invasive spinal surgery (MISS) techniques used for unilateral and dual-channel endoscopic laminectomy. However, limited research has been conducted on lumbosacral multifidus muscle injuries in elderly individuals undergoing MISS for lumbar spinal canal stenosis. The objective of this study was to investigate the impact of single-channel and double-channel MISS on the multifidus muscle in elderly patients.

Identification of hypermucoviscous strains untargeted surface-enhanced Raman spectroscopy.

is one of the most common causes of hospital-acquired infections, especially due to the emergence of the hypervirulent (hvKp) strains. Multiple methods have been developed to discriminate hvKp strains from classical (cKp) strains, such as the presence of candidate genes (, -344, , and ), high level of siderophore production, hypermucoviscosity phenotype, Although the string test is commonly used to confirm the hypermucoviscosity of strains, it is a method lacking rigidity and accuracy. Surface-enhanced Raman spectroscopy (SERS) coupled with machine learning algorithms has been widely used in discriminating bacterial pathogens with different phenotypes.

Prognostic Significance of Tumor Mutation Burden among Patients with Non-small Cell Lung Cancer Who Received Platinum-based Adjuvant Chemotherapy: An Exploratory Study.

This study aimed to investigate the prognostic significance of tumor mutation burden (TMB) among patients with non-small cell lung cancer (NSCLC) who received platinum-based adjuvant chemotherapy. Tumor tissue specimens after surgical resection were collected for DNA extraction. Somatic mutation detection and TMB analysis were conducted using next-generation sequencing (NGS).

Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance.

Helicobacter pylori is a major human pathogen that infects approximately half of the global population and is becoming a serious health threat due to its increasing antibiotic resistance. It is the causative agent of chronic active gastritis, peptic ulcer disease, and gastric cancer and has been classified as a Group I Carcinogen by the International Agency for Research on Cancer. Therefore, the rapid and accurate diagnosis of H.

[Characteristics of VOCs and Formation Potentials of O and SOA in Autumn and Winter in Tongchuan, China].

Volatile organic compounds (VOCs) are the main precursors of tropospheric O and secondary organic aerosol (SOA), which can enhance atmospheric oxidation, promote the formation of secondary pollutants, and affect regional air quality and human health. In order to gain insights on VOCs characteristics and their potentials for O and SOA formation, the volume fraction of 102 VOCs in autumn and winter in the urban area of Tongchuan were monitored using the TH-300B online monitoring system. The maximum incremental reactivity (MIR) coefficient and the fractional aerosol coefficient (FAC) were used to estimate the ozone formation potential (OFP) and SOA formation potential (SOAFP), respectively.

[Bioinformatics analysis, prokaryotic expression and purification of phospholipase A from Neisseria gonorrhoeae].

Objective: To bioinformatically analyze the phospholipase A protein of Neisseria gonorrhoeae, predict its immunogenicity, and express and purify the protein using the prokaryotic system.

Methods: Bioinformatics software was used for sequence analysis and prediction of T cell and B cell epitopes. The phospholipase A gene was amplified by PCR.

Ferroptosis resistance determines high susceptibility of murine A/J strain to iron-induced renal carcinogenesis.

Cancer susceptibility is a critical factor in the understanding of carcinogenesis. Intraperitoneal (i.p.

Microendoscopy-assisted extraforaminal lumbar interbody fusion for treating single-level spondylodesis.

Background: Lumbar interbody fusion is a standard technique for treating degenerative lumbar disorders involving instability. Due to its invasiveness, a minimally invasive technique, extraforaminal lumbar interbody fusion (ELIF), was introduced. On surgically approaching posterolaterally, the posterior muscles and spinal canal are barely invaded.

Scenario analysis of vehicular emission abatement procedures in Xi'an, China.

Vehicular emissions contribute significantly to air pollution, and the number of vehicles in use is continuing to rise. Policymakers thus need to formulate vehicular emission reduction policies to improve urban air-quality. This study used different vehicle control scenarios to predict the associated potential of mitigating carbon monoxide (CO), volatile organic compounds (VOCs), nitrogen oxide (NO), particulate matter with an aerodynamic diameter less than 2.

CT-based radiomics scores predict response to neoadjuvant chemotherapy and survival in patients with gastric cancer.

Background: Neoadjuvant chemotherapy is a promising treatment option for potential resectable gastric cancer, but patients' responses vary. We aimed to develop and validate a radiomics score (rad_score) to predict treatment response to neoadjuvant chemotherapy and to investigate its efficacy in survival stratification.

Methods: A total of 106 patients with neoadjuvant chemotherapy before gastrectomy were included (training cohort: n = 74; validation cohort: n = 32).

From a blue to white to yellow emitter: a hexanuclear copper iodide nanocluster.

Highly emissive copper(i) halide nanoclusters showing thermally activated delayed fluorescence (TADF) have been paid much attention, but rarely reported so far. Herein, a hexanuclear copper(i) iodide cluster containing a tridentate N∧P∧N ligand, [CuI(ppda)] {ppda = 2-[2-(dimethylamino)phenyl(phenyl)phosphino]-N,N-dimethylaniline}, was synthesized. All six copper atoms are four-coordinate, including four CuPNI and two CuI units.

Augmented oxidative stress increases 8-oxoguanine preferentially in the transcriptionally active genomic regions.

8-Oxoguanine (8-oxoG) is the most common DNA base modification in the mammalian genome, associated with oxidative stress. Here we analysed the alterations in the distribution of 8-oxoG across the entire murine genome, before and after an elevation of oxidative stress by the use of ferric nitrilotriacetate (Fe-NTA) as an oxidative stress inducer in the renal proximal tubules. We isolated DNA fragments containing 8-oxoGs with immunoprecipitation from the murine genome, and amplified them by PCR for a distribution analysis with microarray-based comparative genomic hybridisation.

Prevalence and correlation of anxiety and depression on the prognosis of postoperative non-small-cell lung cancer patients in North China.

Identify the prevalence of postoperative anxiety and depression as well as their correlations with clinical features and survival profiles in non-small-cell lung cancer (NSCLC) patients who underwent resection.Four hundred NSCLC patients who underwent resection were recruited, and their anxiety and depression were assessed by hospital anxiety and depression scale (HADS) at discharge after surgery. Besides, 480 healthy controls (HCs) were also enrolled and assessed by HADS.

From deep blue to green emitting and ultralong fluorescent copper(i) halide complexes containing dimethylthiophene diphosphine and PPh ligands.

Highly emissive copper(i) halide complexes having thermally activated delayed fluorescence (TADF) have been paid much attention. Here, a series of four-coordinate mononuclear copper(i) halide complexes containing both bi- and mono-phosphine ligands, [CuX(dpmt)(PPh)] (dpmt = 3,4-bis(diphenylphosphino)-2,5-dimethylthiophene, X = I (1), Br (2) and Cl (3)), were synthesized, and their molecular structures and photophysical properties were investigated. These complexes exhibit intense deep blue emission in crystal state at room temperature and have peak wavelengths at 447-460 nm with microsecond lifetimes (τ = 114-752 μs).

Comparative Transcriptome Analysis Reveals Differentially Expressed Genes and Signaling Pathways Between Male and Female Red-Tail Catfish (Mystus wyckioides).

Sexual dimorphism is widespread in fish species. The red-tail catfish (Mystus wyckioides) is a commercially important catfish in the lower reaches of the Lancang River and the Mekong basin, and it shows a growth advantage in males. Here, RNA-seq was for the first time used to explore the gene expression difference between the sexes in the hypothalamus and pituitary of red-tail catfish, respectively.

Near-saturated red emitters: four-coordinate copper(i) halide complexes containing 8-(diphenylphosphino)quinoline and 1-(diphenylphosphino)naphthalene ligands.

Recently, highly emissive neutral copper halide complexes have received much attention. Here, a series of four-coordinate mononuclear Cu(i) halide complexes, [CuX(dpqu)(dpna)] (dpqu = 8-(diphenylphosphino)quinoline, dpna = 1-(diphenylphosphino)naphthalene, X = I (1), Br (2) and Cl (3)), were synthesized, and their molecular structures and photophysical properties were investigated. These complexes exhibit near-saturated red emission in the solid state at room temperature and have peak emission wavelengths at 669-691 nm with microsecond lifetimes (τ = 0.

Superiority of rat over murine model for studies on the evolution of cancer genome.

Evolution of the species and carcinogenesis are similar in that genomic alterations are the key events. Oxidative stress derived from various etiologies is one of the major causes of carcinogenesis by inducing mutations in the genome. Persistent oxidative stress in the renal proximal tubules through Fenton reaction catalysed by ferric nitrilotriacetate (Fe-NTA) generates renal cell carcinoma (RCC) in mice and rats.

Fenton reaction-induced renal carcinogenesis in Mutyh-deficient mice exhibits less chromosomal aberrations than the rat model.

Oxidative stress including iron excess has been associated with carcinogenesis. The level of 8-oxoguanine, a major oxidatively modified base in DNA, is maintained very low by three distinct enzymes, encoded by OGG1, MUTYH and MTH1. Germline biallelic inactivation of MUTYH represents a familial cancer syndrome called MUTYH-associated polyposis.

Pain-reducing anesthesia prevents oxidative stress in human term placenta.

Anesthesia is sometimes used for the reduction of maternal pain in normal human term labor, but whether the drugs affect oxidative stress remains unclear. The placenta serves as an interface between the maternal and fetal vasculature. In this study, we immunohistochemically analyzed two markers for oxidative stress, namely 8-hydroxy-2'-deoxyguanosine (8-OHdG) and 4-hydroxy-2-nonenal-modified proteins (HNE), using placentas from 21 cases of normal tansvaginal delivery (V group), 20 Caesarean sections (C group), and 17 normal transvaginal deliveries with epidural anesthesia (E group).

Synthesis and photoelectric properties of new Dawson-type polyoxometalate-based dimeric and oligomeric Pt(II)-acetylide inorganic-organic hybrids.

A new synthesis route for preparing Dawson-type polyoxometalate (POM) based inorganic-organic hybrid materials is presented. Two new heteropolytungstate-based dimeric and oligomeric Pt(II) acetylide inorganic-organic hybrid compounds (2PtOD and PPtOD) were prepared by Hagihara's dehydrohalogenating coupling of a terminal diacetylene POM hybrid containing diphosphoryl functionality and an appropriate platinum(II) halide precursor. This method provides a rigid covalent linkage between the POM and the organometallic Pt(II) acetylide moiety.

New Zn²⁺ coordination polymers constructed from acylhydrazidate molecules: synthesis and structural characterization.

By employing two types of hydrothermal in situ ligand reactions (acylation of N2H4 with aromatic polycarboxylic acids, reduction of 3-nitrophthalhydrazide by N2H4), three new acylhydrazidate-extended Zn(2+) coordination polymers [Zn2(3-apth)(atrz)2] (3-apth = 3-aminophthalhydrazidate; atrz = 3-amino-1,2,4-triazolate) 1, [Zn2(4-apth)(atez)2] (4-apth = 4-aminophthalhydrazidate; atez = 5-aminotetrazolate) 2, and [Zn(3-cppth)(H2O)] (3-cppth = 4-(3-carboxyphenoxy)phthalhydrazidate) 3 were obtained. X-ray single-crystal diffraction analysis revealed that (i) compound 1 possesses a 3-D structure. The triazolate molecules link the Zn(2+) ions to form a 2-D layer with a (6,3) topology.

Construction of acylhydrazidate-extended metal-organic frameworks.

Under hydrothermal conditions, the reactions of Ba(2+)/Zn(2+), aromatic polycarboxylic acids and N2H4 with or without oxalic acid were carried out, affording four new acylhydrazidate-extended metal-organic frameworks (MOFs) [Ba(pmdh)] (pmdh = pyromellitdihydrazidate) 1, [Ba(sdpth)(H2O)2]·0.5H2O (sdpth = 4,4'-sulfoyldiphthalhydrazidate) 2, [Ba2(cpth)2(H2O)2] (cpth = 4-carboxylphthalhydrazidate) 3 and [Zn2(pdh)2(ox)]·H2O (ox = oxalate, pdh = pyridine-2,3-dicarboxylhydrazidate) 4. The acylhydrazidate molecules pmdh, sdpth, cpth and pdh in compounds 1-4 derived from the hydrothermal in situ acylation of N2H4 with aromatic polycarboxylic acids.

New thiocyanatocadmates templated by multi-dentate N-heterocyclic/diamine molecules.

With diverse multi-dentate N-heterocyclic/diamine molecules as the cations, the reactions between Cd(2+) and SCN(-) in C2H5OH/H2O solution acidified by H2SO4 created five new thiocyanatocadmates [H2(bim)][Cd(SCN)2(H2O)2]SO4 (bim = 2,2'-biimidazole) 1, [H2(pympip)]2[Cd2(SCN)4(SO4)2(H2O)4]·2H2O (pympip = 1-(2-pyrimidyl)piperazine) 2, [H2(tdpy)]2[Cd3(SCN)10] (tdpy = 4,4'-thiodipyridine) 3, [H2(badpm)][Cd(SCN)4] (badpm = 4,4'-biaminodiphenylmethane) 4 and [H2(pdma)][Cd2(SCN)4(SO4)] (pdma = 1,4-phenylenedimethanamine) 5. It is noteworthy that (i) in compounds 1, 2 and 5, SO4(2-) was incorporated into the organically templated thiocyanatocadmate frameworks; (ii) compound is a rare example, in which two types of chained thiocyanatocadmates are found, and (iii) compound shows an interesting layer structure, constructed up from the ribboned thiocyanatocadmates by μ2-mode SO4(2-). Photoluminescence analysis revealed that compounds 1, 2 and 5 in solid state emit blue light with the maximum at 425 nm for 1, 405 nm for 2 and 445 nm for 5, respectively.

New metal complexes with di(mono)acylhydrazidate molecules.

The simple hydrothermal self-assemblies between metal salts, organic polycarboxylic acids and N(2)H(4)·H(2)O, sometimes in the presence of phenanthroline (phen), created four di(mono)acylhydrazidate-coordinated compounds [Pb(2)(DPHKH)(2)(phen)(2)]·2H(2)O 1, [Cd(ODPTH)(phen)]·0.25H(2)O 2, [Pb(2)(MPTH)(4)(phen)(2)] 3, [Cd(2)(MPTH)(4)(phen)(2)]·H(2)O 4 as well as one diacylhydrazide molecule [H(2)(ODPTH)] 5 (DPHKH = 4,4'-diphthalhydrazidatoketone hydrazone, MPTH = 3-methylphthalhydrazidate, ODPTH = 4,4'-oxydiphthalhydrazidate). Note that the di(mono)acylhydrazidate molecules in the title compounds originated from the in situ acylation reactions between organic polycarboxylic acids and N(2)H(4)·H(2)O.

[Prenatal diagnosis of β-thalassaemia using cell-free fetal DNA in maternal plasma].

Objective: To investigate the clinical feasibility of cell-free fetal DNA (cffDNA)-based noninvasive prenatal diagnosis of β-thalassemia.

Methods: Nine samples of amniotic fluid were obtained to detect the 8 common and 9 relatively rare mutation sites of β-thalassaemia in Guangdong Province. The maternal blood samples were also collected for extracting and purification of the cffDNA, and a duplex PCR was performed using 3 pairs of primers and the fetal β-globin genotype was analyzed by reverse dot-blot hybridization.