Single-Cell RNA Sequencing Reveals Cellular and Transcriptional Changes Associated With Traumatic Brain Injury.

Traumatic brain injury (TBI) is currently a substantial public health problem and one of the leading causes of morbidity and mortality worldwide. However, the cellular and transcriptional changes in TBI at single-cell level have not been well characterized. In this study, we reanalyzed a single-cell RNA sequencing (scRNA-seq) dataset of mouse hippocampus to identify the key cellular and transcriptional changes associated with TBI.

[Exploration and characterization of a universal piggyBac transposon vector for efficient transgene studies in sheep].

piggyBac (PB) is a DNA transposon that mediates transposition in various animal cells. As an efficient tool, PB transposons are applied to various transgenic research and optimized for different species, which is an essential means to enhance its versatility. To construct a universal PB transposase (PBase) vector for ovine transgene manipulation, the gene expression box of PBase with optimized bias for sheep codons was inserted into the pBNW-TP1 vector.

[Detection and sequences analysis of bovine hepatitis E virus RNA in Xinjiang Autonomous Region].

Reverse transcription-nested polymerase chain reaction (RT-nPCR) was used to detect HEV (Hepatitis E virus, HEV) RNA in dung or anus-swab samples collected from the cows with the positive anti-HEV antibodies in dairy farms in Xinjiang Autonomous Region. 7 of 60 (11.67%) cows were positive for HEV RNA in one farm.