N6-methyladenosine modification of hypoxia-inducible factor-1α regulates -associated gastric cancer the PI3K/AKT pathway.

Background: () colonizes the human gastric mucosa and is implicated in the development of gastric cancer (GC). The tumor microenvironment is characterized by hypoxia, where hypoxia-inducible factor-1α (HIF-1α) plays a key role as a transcription factor, but the mechanisms underlying -induced HIF-1α expression and carcinogenesis remain unclear.

Aim: To explore the underlying mechanism of -induced HIF-1α expression in promoting the malignant biological behavior of gastric epithelial cells (GES-1).

Role of Renin-Angiotensin System in Acute Lung Injury Caused by Viral Infection.

The renin-angiotensin system (RAS) is the most important regulatory system of electrolyte homeostasis and blood pressure and acts through angiotensin-converting enzyme (ACE)/angiotensin II (Ang II)/Ang II type 1 (AT1) receptor axis and angiotensin-converting enzyme 2 (ACE2)/angiotensin (1-7)/MAS receptor axis. RAS dysfunction is related to the occurrence and development of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) and causes a serious prognosis and even death. ALI/ARDS can be induced by various ways, one of which is viral infections, such as SARS-CoV, SARS-CoV-2, H5N1, H7N9, and EV71.

Emergence and mechanism of carbapenem-resistant Escherichia coli in Henan, China, 2014.

The emergence and dissemination of carbapenem-resistant Escherichia coli (E. coli) strains is a main risk for global public health, but little is known of carbapenemase producing E. coli in Henan, China.

Pathogenic mechanisms of the oncoprotein CagA in H. pylori-induced gastric cancer (Review).

Infection with Helicobacter pylori is the strongest risk factor for the development of chronic gastritis, gastric ulcer and gastric carcinoma. The majority of the H. pylori-infected population remains asymptomatic, and only 1% of individuals may progress to gastric cancer.

Role of Helicobacter pylori infection in pathogenesis of gastric carcinoma.

Gastric cancer (GC) is one of the most common carcinoma and the second leading cause of cancer-related deaths worldwide. Helicobacter pylori (H. pylori) infection causes a series of precancerous lesions like gastritis, atrophy, intestinal metaplasia and dysplasia, and is the strongest known risk factor for GC, as supported by epidemiological, preclinical and clinical studies.

Incidence, survival and prevalence of esophageal and gastric cancer in Linzhou city from 2003 to 2009.

This study describes recent trends in incidence, survival and prevalence of subgroups of esophageal and gastric cancer in Linzhou city between 2003 and 2009. Data of esophageal and gastric cancer for the period of interest were extracted from the Linzhou Cancer Registry. Using information on tumor morphology or anatomical site, data were divided into six groups; esophageal squamous cell carcinoma, esophageal adenocarcinoma, other and unspecified types of esophageal cancer, and cardia, non-cardia, and unspecified anatomical site of stomach cancer.

Prognostic impact of cyclin D1, cyclin E and P53 on gastroenteropancreatic neuroendocrine tumours.

Conventional classifications of gastroenteropancreatic neuroendocrine tumours (GEP- NETs) are rather unsatisfactory because of the variation in survival within each subgroup. Molecular markers are being found able to predict patient outcome in more and more tumours. The aim of this study was to characterize the expression of the proteins cyclin D1, cyclin E and P53 in GEP- NETs and assess any prognostic impact.

How often are ineffective interventions still used in clinical practice? A cross-sectional survey of 6,272 clinicians in China.

Background: The World Health Organization reported in 2011 that irrational use of medicines was a serious global problem that is wasteful and harmful. The worst is use of ineffective or harmful interventions which should not be used at all. However, little is known about the changes that 20 years of evidence-based medicine has made particularly in reducing use of ineffective interventions.

Transgenic peanut (Arachis hypogaea L.) expressing the urease subunit B gene of Helicobacter pylori.

Helicobacter pylori (H. pylori) has been identified as the main pathogenic factors of chronic gastritis and peptic ulcer, and the Class I carcinogen of gastric cancer by WHO. Vaccine has become the most effective measure to prevent and cure H.

Atypical class 1 integron coexists with class 1 and class 2 integrons in multi-drug resistant Shigella flexneri isolates from China.

The antimicrobial resistance and the character of integrons were determined in 58 Shigella flexneri strains isolated from China. All isolates were multi-drug resistant and found to carry integrons of class 1 (94.8%), class 2 (100%), or both (94.

Mutation of iceA in Helicobacter pylori compromised IL-8 induction from human gastric epithelial cells.

IceA of Helicobacter pylori (H. pylori) has been suggested as a virulence factor for the bacteria, but its pathogenic role remains unelucidated. Here, we examined the effect of iceA mutation on the secretion of IL-8 by human gastric epithelial cells.

Mutation of cytotoxin-associated gene A affects expressions of antioxidant proteins of Helicobacter pylori.

Aim: To determine if disruption of the cagA gene of Helicobacter pylori (H pylori) has an effect on the expression of other proteins at proteome level.

Methods: Construction of a cagA knock out mutant Hp27_Delta cagA (cagA(-)) via homologous recombination with the wild-type strain Hp27 (cagA(+)) as a recipient was performed. The method of sonication-urea-CHAPS-DTT was employed to extract bacterial proteins from both strains.

[Construction, expression and purification of UreB-Omp11 fusion protein of Helicobacter pylori and its immunocompetence].

Aim: To construct H.pylori vaccine candidate strain expressing UreB-Omp11 recombinant fusion protein of H.pylori.

[The mechanisms and effects of lutein on inducing the cell differentiation of human esophagus cancer EC9706].

Objective: The purpose of the study was to explore the effects and molecular mechanisms of lutein on the differentiation of esophagus cancer EC9706 cell.

Methods: EC9706 cells were seeded in 1640 medium before the addition of test compounds. The respective test compound was added in fresh medium and the control cell received the vehicle (DMSO) or Fluorouracil.

[Effects and mechanism of lutein on apoptosis of esophageal carcinoma EC9706 cells].

Objective: To study the effects of lutein on apoptosis and its mechanism.

Method: The cells of human esophageal carcinoma EC9706 were grown in RPMI medium containing 10% bovine serum and were treated with lutein at 100 microg x mL(-1) concentration. Flow cytometry was employed to investigate the effects of lutein on cell apoptosis of EC9706 cells.

[Study on the level changes of obesity-associated factors and puberty-associated hormones and their relationship between obese and normal 13 years old children].

Objective: To investigate the level changes of obesity-associated factors and puberty-associated hormones and their relationship between obese and normal Children.

Methods: The concentrations of orexins, leptin, insulin, testosterone (T), estradiol (E), luteotrophic hormone (LH), as well as follicle stimulating hormone (FSH) in blood were measured respectively in 78 cases of 13 years old obesity children and 84 normal children as a control group by the means of RIA methods. The bone ages were evaluated with "bone age standard of hundred percent scale".

[Blood leptin, orexins and NPY levels and their relations in obese children].

Objective: To investigate the concentration levels of leptin, orexins and neuropeptide Y (NPY) in the blood of obese children, and to analysed the relationship between these substances.

Methods: RIA methods were used to measure the concentrations of leptin, orexinA, orexinB, and NPY in the blood of 98 obese children [BMI: male (29.24 +/- 1.

Fusion expression of Helicobacter pylori neutrophil-activating protein in E.coli.

Aim: To produce a recombinant protein rMBP-NAP, which was fusionally expressed by Helicobacter pylori (H pylori) neutrophil-activating protein (NAP) and E. coli maltose-binding protein (MBP) and to evaluate its immunoreactivity and immunogenicity.

Methods: Neutrophil-activating protein gene of H pylori (HP-napA) was subcloned from the recombinant plasmid pNEB-napA, and fused to MalE gene of expressing vector pMAL-c2x.

[Study on the molecular mechanism of quinolone resistance in Shigellae spp].

Objective: To study the resistance and its mechanism of Shigellae spp. to quinolones.

Methods: Seventy-three clinical isolates were collected.