Publications by authors named "Guang Jian Huang"
Article Synopsis
- The CRNDE gene, a long noncoding RNA, is found to be increased in gastric cancer and may play a role in regulating cell growth.
- Research methods included assessing expression levels through qRT-PCR and examining cell viability with MTT assays, revealing that higher CRNDE levels correlated with increased growth and colony formation of gastric cancer cells.
- The study highlights CRNDE's interaction with the miR-145 and its target E2F3, suggesting that CRNDE could be a potential target for gastric cancer therapies.
JMJD2A predicts prognosis and regulates cell growth in human gastric cancer.
Biochem Biophys Res Commun
June 2014
A number of JmjC domain-containing histone demethylases have been identified and biochemically characterized in mammalian. JMJD2A is a transcriptional cofactor and enzyme that catalyzes demethylation of histone H3 lysines 9 and 36. Here in this study, we aim to explore the role of JMJD2A in human gastric cancer.
View Article and Find Full Text PDFThe RNA-binding protein PCBP2 facilitates gastric carcinoma growth by targeting miR-34a.
Biochem Biophys Res Commun
June 2014
Article Synopsis
- Gastric carcinoma is the fourth most prevalent cancer globally, often resulting in high mortality and low survival rates; however, its underlying mechanisms remain unclear.
- The study highlights RNA-binding protein PCBP2 as a key oncogenic factor in gastric cancer, found to be elevated in cancer tissues and linked to poorer survival outcomes.
- Investigating further, the research indicates that suppressing PCBP2 reduces cancer cell growth and promotes cell death by influencing the balance of pro-apoptotic and anti-apoptotic proteins through the regulation of miR-34a.
Comprehensive analysis of vascular endothelial growth factor-C related factors in stomach cancer.
Asian Pac J Cancer Prev
December 2014
Background: Vascular endothelial growth factor-C (VEGF-C), which contributes to lymphatic metastasis (LM) in malignant disease, is one of the most important factors involved in physical and pathological lymphangiogenesis. Some VEGF-C related factors such as sine oculis homeobox homolog (SIX) 1, contactin (CNTN) 1 and dual specificity phosphatase (DUSP) 6 have been extensively studied in malignancies, but their expression levels and associations have still to be elucidated in stomach cancer.
Methods: We detected their expression levels in 30 paired stomach cancer tissues using quantitative real-time reverse transcription-PCR (qRT-PCR).
Objective: Defective immune function is an important cause of tumor development. Accumulation of myeloid-derived suppressor cell (MDSC) associated with inhibition of dendritic cell (DC) function is one of the major immunological abnormalities in cancer. However, the molecular mechanism of the phenomenon remains unclear.
View Article and Find Full Text PDFObjective: To observe the pharmacokinetics of adriamycin-adsorbing nanometric activated carbon in intralymphatic chemotherapy.
Methods: Two ml of suspension of activated carbon with the diameter of 21 nm was mixed with adriamycin 5 mg. Eighteen dogs were randomly divided into 6 equal groups.
Objective: To observe the effect of injecting activated carbon ultramicroparticles around the gastric tumor before or during operation on staining lymph nodes and guiding the lymphadenectomy of gastric cancer.
Methods: Forty-three cases of gastric cancer received activated carbon (AC) ultramicroparticles around the tumor by submucosal endoscopic injection 1 approximately 6 days before the operation and/or intraoperative subserosal injection (AC group), whereas 82 cases of gastric cancer without the injection were used as control group. The number of dissected lymph nodes, number of black-stained lymph nodes and its relation to the injection time, metastasis of lymph nodes, and the side effect of the procedure were analyzed.
Objective: To evaluate the effect of a novel blockade technique for gastric cancer on blood-borne metastasis of gastric cancer cells to portal vein.
Methods: Twenty-three cases of gastric cancer were divided into routine operation group (8 cases intraoperatively without blockade technique) and blockade group (15 cases with blockade technique). Blood samples from portal vein pre- and intraoperatively, as well as gastroepiploic vein limited within the blockade area were obtained to detect CK19 mRNA expression by using RT-PCR technique.