Prevascularized Hydrogel Enhancing Innervation and Repair of Full-Thickness Volumetric Muscle Loss in Abdominal Wall Defects.

Current materials for repairing abdominal peritoneal defects face rapid degradation, infection risk, insufficient vascular ingrowth, slow muscle regeneration, and suboptimal postoperative integration, often causing fibrotic healing and hindering volumetric muscle loss (VML) repair exceeding 30%. To address these issues, photo-cross-linkable gelatin hydrogels are combined with blood vessel-forming cells to reconstruct vascular networks, providing temporary nutrient and gas channels that support cell repair. By developing a polymer-chain propagation time technique, hydrogel properties are optimized, avoiding limitations of conventional light exposure.

STING Deficiency Promotes Th17-Like Tfh to Aggravate the Experimental Autoimmune Uveitis.

Purpose: The purpose of this study was to explore the underlying mechanism that Th17-like T follicular helper cells (Tfh) orchestrated by STING signaling have a pathogenic role in experimental autoimmune uveitis (EAU).

Methods: The differences of transcriptome and gene ontology (GO) pathway of Tfh between EAU and control mice were analyzed by single-cell RNA sequence (scRNA-seq) and bulk RNA sequence. Additionally, draining lymph nodes (DLNs) were extracted to verify the expression of IL-17A and IFN-γ in Tfh from EAU and control mice by flow cytometry.

Environmental pollution evaluation and spatiotemporal distribution of different forms of phosphorus in a phosphate mining area in Western Hubei, China.

Taking the typical phosphate mining area in Western Hubei as the research object, groundwater, surface water, soil and sediment in phosphate mining area were investigated and evaluated comprehensively to clarify the temporal and spatial distribution characteristics of different forms of phosphorus in phosphate mining area and reveal the migration and transformation of phosphorus. Results showed that whether in the dry season or the wet season, total phosphorus levels in groundwater (dry season from November to December 2021, 0.42 mg/L; wet season from August to September 2022, 0.

Development of a Cascade-Targeting Oral Vaccine via Glycoprotein 2 on Intestinal Microfold Cells for Cancer Immunotherapy.

Oral cancer vaccines are convenient and safe, with the presence of gut-associated lymphoid tissue (GALT) involved intestinal Peyer's patch (PPs) containing microfold (M) cells and housing abundant underneath dendritic cells (DCs). Here, we found that the endocytic receptors glycoprotein 2 (GP2) and dectin-1 are respectively expressed on M cells and DCs with high specificity. Then, we discovered a gastrointestinal hydrolysis-resistant D-peptide GPBP-2(1-8) targeting GP2 by phage display screening and optimization.

A high-capacity combination of Pluronic L64-Cupping for intramuscular gene delivery.

Intramuscular injection of plasmid DNA (pDNA) is a promising approach for gene therapy, but its efficiency is hindered by both extracellular and intracellular barriers. The extracellular matrix (ECM), including collagens and nucleases, obstructs pDNA penetration, while intracellular challenges include crossing the plasma membrane, escaping endosomes, and reaching the nucleus. Though non-viral carriers like polymers and cationic lipids have been developed, they often fail to address both barriers simultaneously, leading to poor gene transfer in vivo.

A covalent peptide-based lysosome-targeting protein degradation platform for cancer immunotherapy.

The lysosome-targeting chimera (LYTAC) strategy provided a very powerful tool for the degradation of membrane proteins. However, the synthesis of LYTACs, antibody-small molecule conjugates, is challenging. The ability of antibody-based LYTACs to penetrate solid tumor is limited as well, especially to cross the blood-brain barrier (BBB).

Integrated photonic crystal beam splitters based on thin film lithium niobate.

We propose and demonstrate integrated photonic crystal (PhC) beam splitters based on X-cut thin film lithium niobate (TFLN). Its working principle is based on bandgap guidance and total reflection in the PhC slab. We designed two structures: one features a triangular lattice, while the other exhibits a tetragonal lattice.

Silibinin, a PLC-β3 inhibitor, inhibits mast cell activation and alleviates OVA-induced asthma.

The immunoglobulin E (IgE) receptor FcεRI (Fc epsilon RI) plays a crucial role in allergic reactions. Recent studies have indicated that the interaction between FcεRIβ and the downstream protein phospholipase C beta 3 (PLCβ3) leads to the production of inflammatory cytokines. The aim of this study was to develop small molecules that inhibit the protein-protein interactions between FcεRIβ and PLCβ3 to treat allergic inflammation.

Brussels Chicory Enhances Exhaustive Aerobic Exercise Performance and Post-Exercise Recovery, Possibly Through Promotion of Lactate Oxidation: A Pilot Randomized, Single-Blind, Placebo-Controlled, Two-Way Crossover Study.

Background: Brussels chicory affluent in phenolic acids could inhibit atherosclerosis; however, its effects on exercise performance and post-exercise recovery are unknown. We hypothesized that Brussels chicory could enhance exhaustive aerobic exercise performance and post-exercise recovery by promoting lactate oxidation.

Methods: This is a single-blind, randomized, placebo-controlled two-way cross-over trial involving 32 untrained college students (men 18) who consumed either Brussels chicory juice (100 g of Brussels chicory containing ~130 mg phenolic acids and 180 mL fresh milk) or placebo (180 mL fresh milk) for 7 days with a 2-week washout period.

Environmental Exposure to Bisphenol A Enhances Invasiveness in Papillary Thyroid Cancer.

Bisphenol A (BPA) is a prevalent environmental contaminant found in plastics and known for its endocrine-disrupting properties, posing risks to both human health and the environment. Despite its widespread presence, the impact of BPA on papillary thyroid cancer (PTC) progression, especially under realistic environmental conditions, is not well understood. This study examined the effects of BPA on PTC using a 3D thyroid papillary tumor spheroid model, which better mimicked the complex interactions within human tissues compared to traditional 2D models.

Neutrophil extracellular traps potentiate effector T cells via endothelial senescence in uveitis.

Autoimmune uveitis (AU) is a sight-threatening ocular autoimmune disorder that often manifests as retinal vasculitis. Increased neutrophil infiltration around retinal vessels has been reported during the progression of AU, while how they function is not fully recognized. Neutrophil extracellular traps (NETs), produced by activated neutrophils, have been suggested to be detrimental in autoimmune diseases.

Circadian Rhythm Disruption Exacerbates Autoimmune Uveitis: The Essential Role of PER1 in Treg Cell Metabolic Support for Stability and Function.

Circadian rhythm plays a critical role in the progression of autoimmune diseases. While our previous study demonstrated the therapeutic effects of melatonin in experimental autoimmune uveitis, the involvement of circadian rhythm remained unclear. Using a light-induced circadian rhythm disruption model, we showed that disrupted circadian rhythms exacerbate autoimmune uveitis by impairing the stability and function of Treg cells.

Effects of exosomes from human dental pulp stem cells on the biological behavior of human fibroblasts.

The aim of this study was to investigate the effect of dental pulp stem cell-derived exosomes (DPSCs-Exos) on the biological behaviour of fibroblasts, particularly on keloid fibroblasts (KFs) and normal skin fibroblasts (NFs), with a view to providing new insights into cellular regenerative medicine. We obtained DPSCs-Exos by ultracentrifugation and co-cultured it with KFs and NFs. We detected its effect on cell proliferation using the CCK-8 assay; cell migration ability by cell scratch and Transwell assays; extracellular matrix synthesis using the hydroxyproline content assay; the expression levels of genes associated with fibrosis by PCR assay; and the expression levels of proteins related to fibrosis in the cells using the Western Blot method.

Inhalable Mucociliary-On-Chip System Revealing Pulmonary Clearance Dynamics in Nanodrug Delivery.

The development of a inhaled nanodrug delivery assessment platform is crucial for advancing treatments for chronic lung diseases. Traditional in vitro models and commercial aerosol systems fail to accurately simulate the complex human respiratory patterns and mucosal barriers. To address this, we have developed the breathing mucociliary-on-a-chip (BMC) platform, which replicates mucociliary clearance and respiratory dynamics in vitro.

Revolutionizing Epithelial Differentiability Analysis in Small Airway-on-a-Chip Models Using Label-Free Imaging and Computational Techniques.

Organ-on-a-chip (OOC) devices mimic human organs, which can be used for many different applications, including drug development, environmental toxicology, disease models, and physiological assessment. Image data acquisition and analysis from these chips are crucial for advancing research in the field. In this study, we propose a label-free morphology imaging platform compatible with the small airway-on-a-chip system.

Hydrogen-Generating Magnesium Alloy Seed Strand Sensitizes Solid Tumors to Iodine-125 Brachytherapy.

Radioactive iodine-125 (I) seed implantation, a brachytherapy technique, effectively kills tumor cells via X-rays and gamma rays, serving as an alternative therapeutic option following the failure of frontline treatments for various solid tumors. However, tumor radioresistance limits its efficacy. Hydrogen gas has anticancer properties and can enhance the efficacy of immunotherapy.

Degradable chiral mesoporous silica nanoparticles and carboxymethyl chitosan/cystamine hydrogels for selective loading and controlled release of S-naproxen.

Degradable chiral mesoporous silica nanoparticles (DCMSN) are synthesized for selective loading and controlled release of S-naproxen (S-NPX). Chiral silane coupling agent (APTES-L) and degradable silane coupling agent (APTES-CN) are synthesized, respectively, which are used for the synthesis of DCMSN. APTES-L endows the DCMSN with chirality, while APTES-CN endows the DCMSN with degradability.

Dual amplified electrochemical sensing coupling of ternary hybridization-based exosomal microRNA recognition and perchlorate-assisted electrocatalytic cycle.

Exosomal microRNA (miRNA) are important biomarkers for liquid biopsy, and display clinical molecular signatures for cancer diagnosis. Although advanced detection methods have been established to detect exosomal miRNAs, they are faced with certain challenges. Therefore, we aimed to establish a dual amplification-based electrochemical method for detecting exosomal miRNA.

Dexmedetomidine decreases cerebral hyperperfusion incidence following carotid stenting: A double-blind, randomized controlled trial.

Background: Cerebral hyperperfusion syndrome (CHS) is a severe complication after carotid artery stenting (CAS). Dexmedetomidine (Dex) is an α adrenoceptor agonist with sedative, analgesic, and neuroprotective properties. This randomized, double-blind, placebo-controlled trial (ChiCTR1900024416) aims to investigate whether prophylactic low-dose Dex decreases CH-induced brain injury following CAS.

Electrochemical Switching of Electromagnetism by Hierarchical Disorder Tailored Atomic Scale Polarization.

High-frequency electronic response governs a broad spectrum of electromagnetic applications from radiation protection, and signal compatibility, to energy recovery. Despite various efforts to manage electric conductivity, dynamic control over dielectric polarization for real-time electromagnetic modulation remains a notable challenge. Herein, an electrochemical lithiation-driven hierarchical disordering strategy is demonstrated for actively modulating electromagnetic properties.

The Synergetic Effect of Metal-Loaded Electrospun Carbon Fibers for Photothermal Conversion.

With the increasing demand for energy and worsening environmental issues, the application of photothermal materials has been widely explored due to their high energy conversion capabilities and environmental friendliness. In this work, metal-carbon fiber composites were prepared and subjected to photothermal and water evaporation performance tests alongside pure metals and pyrolytic phenolic resin materials. The results show that the addition of metals effectively improved the photothermal efficiency by narrowing the molecular energy gaps of the materials, indicating a strong synergistic enhancement effect between metals and carbon materials.

Enzymatic bioremediation of polycyclic aromatic hydrocarbons (PAHs) in contaminated soil: a study on the recombinant laccase TVL.

Polycyclic aromatic hydrocarbons (PAHs) are pervasive and persistent pollutants in contaminated soil, posing a severe health and environmental threat. Enzymatic bioremediation presents a viable solution for the remediation of PAH-contaminated soil. In this study, a recombinant laccase with the encoding gene originating from and recombinantly expressed in designated as TVL was discovered to possess strong PAH reduction capabilities.

PoCXCL8, a teleost chemokine, exerts direct bactericidal, chemotactic/phagocytic, and NETs releasing properties, promoting host anti-bacterial immunity.

As an important CXC chemokine, CXCL8 plays pleiotropic roles in immunological response. In teleost, CXCL8 is involved in cell migration and bacterial invasion. However, the immune antibacterial function of CXCL8 in Japanese flounder (Paralichthys olivaceus) (PoCXCL8) is largely scarce.