[The effects of the Smad3-knockout on the hematopoiesis of mouse].

The effects of the Smad3- knockout on the hematopoiesis of mouse were investigated in this work. Five pairs of wild type and Smad3- null mice were studied. White blood cell(WBC), red blood cell(RBC) and platelet (PLT) counting of peripheral blood cells were performed with blood obtained from tails.

[Dendritic cells (DC) induced from acute myeloid leukemia (AML) cells with cytokine cocktails].

Objectives: To explore the feasibility of DC being in vitro induced from AML cells with cytokine cocktails and their biological properties.

Methods: AML cells were cultured in either presence or absence of cytokine cocktails. DC were studied for morphology, and cytochemical and immunofluorescent staining.

[Identification and isolation of mesenchymal stem cells from human fetal pancreas].

Objective: To isolate and identify the phenotype and biological characteristics of pancreas derived mesenchymal stem cell.

Methods: Fresh pancreas of 4-5 months old aborted fetus was dissected free from connective tissue, and was cut into small pieces. The adherent cells were harvested and subcultured, after the third subculture, the cells were used for examination.

[Transplantation of mesenchymal derived stem cells followed by G-CSF injection can reconstitute hematopoiesis of lethally irradiated BALB/c mice].

Objective: To explore the hematopoietic reconstitution potential of mesenchymal derived stem like cells.

Methods: We transplanted bone marrow mesenchymal derived stem like cells into lethally irradiated BALB/c mice. Hematopoietic cells were derived from the non-adherent bone marrow cells 24 hours after initial culture while murine mesenchymal derived stem like cells from bone marrow of donor mice were cultured for 10 days before the transplantation.

[Study of biological characteristics of murine dermal mesenchymal stem cells and their enhancement of hematopoiesis recovery].

Objective: To study the characteristics of dermal mesenchymal stem cells (DMSC), and explore whether they could enhance hematopoiesis recovery in vivo as well as facilitate proliferation and differentiation of hematopoietic cells in vitro.

Methods: Multipotential stem cells from the murine dermal mesenchyme were dissociated and cultured as donor cells. After 2 approximately 3 passages, the growth status, cell cycle, immunophenotype and morphology of DMSC were analyzed.

[Phenotypical and Biological Characteristics of Human Fetal Marrow and Liver Mesenchymal Stem Cells].

There has been an increasing interest in recent years in the stromal cell system. The stroma is a complex tissue, composed of a number vascular and connective tissue cell types including endothelial cells, adipocytes, smooth muscle cells, osteogenic cells, and stromal cells. The marrow mesenchymal stem cells are capable of self-renewal and differentiate into various connective tissue lineages including bone, cartilage, tendon, muscle, fat, and marrow stroma.