[New possibilities of the antibiotic use in the treatment of patients with acute cholecystitis].

The authors studied the efficacy of directed transport of antibiotics in autologous blood shadows in the complex treatment of 73 patients with acute cholecystitis (the main group) in comparison with the traditional nonoperative treatment of 67 patients with the same disease (the control group). The directed transport of antibiotics in autologous blood shadows makes it possible to arrest adequately the clinico-laboratory manifestations of acute cholecystitis, thus preventing the development of infectious complications of the disease, shorten by 4.5 times the terms for arresting the clinico-laboratory manifestations of acute cholecystitis, and to optimize planned operative treatment.

[Individual choice of ribonuclease and fluorofur doses in the treatment of patients with acute pancreatitis].

A method is suggested for individual calculation of optimal doses of ribonuclease and fluorofur on the basis of a standardized index--the degree of severity. A CM 1404 computer was used in solving the problem. From analysis of 500 case records by means of programs of discriminant and multidimensional linear regressive analysis a mathematical dependence was found between some parameters indirectly reflecting the severity of the patient's condition, the ribonuclease dose, and the efficacy of the treatment.

Apparent activation of rabbit lung membrane adenylate cyclase by cytosolic proteins possessing adenylate kinase activity.

Lung cytosolic fraction (23500 x g supernatant) activates cAMP synthesis by lung membrane adenylate cyclase (AC). 23 kDa and 29 kDa proteins were isolated from rabbit lung cytosolic fraction in a homogeneous state, as 'activators' of lung membrane AC. Both of these proteins possess high adenylate kinase (AK) activity and are able to mimic the 'activating' effect of lung cytosol on the lung membrane AC in the standard incubation mixture devoid of adenylate kinase.

The family of human Na,K-ATPase genes. ATP1AL1 gene is transcriptionally competent and probably encodes the related ion transport ATPase.

The multigene family of human Na,K-ATPase is composed of 5 alpha-subunit genes, 3 of which were shown to encode the functionally active alpha 1, alpha 2 and alpha 3 isoforms of the catalytic subunits. This report describes the isolation, mapping and partial sequencing of the fourth gene (ATP1AL1) that was demonstrated here to be functionally active and expressed in human brain and kidney. Limited DNA sequencing of the ATP1AL1 exons allowed one to suggest that the gene probably encodes a new ion transport ATPase rather than an isoform of the Na,K-ATPase or the closely related H,K-ATPase.

[Modern aspects of reduction of mortality in acute appendicitis in children under three years of age].

The experience with treatment of 216 young children with acute appendicitis is summarized. All the patients were operated on after the complex preoperative preparation. Median laparotomy was performed in 17 patients, 199 were operated on with local approach.

[Biomechanical substantiation of osteosynthesis of the distal tibiofibular syndesmosis with wire].

On the basis of their biomechanical experimental investigations on 10 metal models and 20 anatomic preparations of the ankle joint the authors have determined the quantitative parameters of the strength of tightening intertibial syndesmosis with a wire loop going round the transversal canals through the fibula and of the tibia. The reliability and the biomechanical validity of the method proposed by the authors have been confirmed by 39 clinical observations. The surgical technique is described.

[Study of expression of Na+,K+-ATPase subunit genes in the human brain using cDNA libraries].

Human brain cDNA libraries were screened with cDNA inserts corresponding to the mRNA for the Na+,K(+)-ATPase alpha-subunit from pig kidney. The results obtained demonstrate the existence of two highly homologous mRNAs encoding the alpha- and alpha III-isoforms of the Na+,K(+)-ATPase catalytic subunit.

[Computed analysis of brain electrograms under the action of psychotropic drugs].

The computed analysis of the EEG-effects of a neuroleptic ethaperazine and an antidepressant melipramine administered in different doses was made in the experiments on cats. It was established that ethaperazine (3 mg/kg) and melipramine (6 mg/kg) increase the spectral power in the range of 1-2 Hz; the maximal effect was noted 2 hours after their oral administration Ethaperazine primarily decreases the powers of the spectra of the hypothalamus and reticulotegmental nucleus in the range of 13-14 Hz and over. Melipramine in a dose of 6 mg/kg decreases the spectral power of the hippocampus in the range of 4-12 Hz, of the mediodosal and caudal nuclei in the range of 8-12 Hz.

Family of Na+,K+-ATPase genes. Intra-individual tissue-specific restriction fragment length polymorphism.

Intra-individual tissue-specific restriction fragment length polymorphism (RFLP) has been demonstrated in DNA isolated from different mammalian tissues using cDNAs of alpha- and beta-subunits of Na+,K+-ATPase as hybridization probes. We propose that the RFLPs could result from gene rearrangements in the gene loci for the alpha- and beta-subunits of Na+,K+-ATPase. The changes in restriction patterns have been shown to occur during embryonic development and tumor formation.

[Primary structure of the alpha-subunit of Na+,K+-ATPase from the swine kidney. III. Complete nucleotide sequence corresponding to the structural region of the gene].

The nucleotide sequence of the cDNA, containing coding region of the alpha-subunit of the pig kidney Na+, K+-ATPase, was determined. The region contains 3063 b.p.

[Primary structure of the beta-subunit of Na+,K+-ATPase from the swine kidney. II. Reverse transcription, cloning of mRNA, complete nucleotide sequence corresponding to the structural region of the gene].

mRNA coding for beta-subunit of Na+, K+-ATPase from pig kidney is about 24-25S as deduced from the hybridization pattern of poly (A)+-RNA with two synthetic oligonucleotides structurally corresponding to two peptides isolated from the tryptic hydrolyzate of beta-subunit. Cloning of cDNA allowed to determine the complete structure of the gene and to deduce the amino acid sequence of beta-subunit of Na+, K+-ATPase. The beta-subunit contains 302 amino acid residues and the protein is not processed at the N-nor at the C-terminus.

Pig kidney Na+,K+-ATPase. Primary structure and spatial organization.

cDNAs complementary to pig kidney mRNAs coding for alpha- and beta-subunits of Na+,K+-ATPase were cloned and sequenced. Selective tryptic hydrolysis of the alpha-subunit within the membrane-bound enzyme and tryptic hydrolysis of the immobilized isolated beta-subunit were also performed. The mature alpha- and beta-subunits contain 1016 and 302 amino acid residues, respectively.