Dynamics of the Glycogen β-Particle Number in Rat Hepatocytes during Glucose Refeeding.

Glycogen is an easily accessible source of energy for various processes. In hepatocytes, it can be found in the form of individual molecules (β-particles) and their agglomerates (α-particles). The glycogen content in hepatocytes depends on the physiological state and can vary due to the size and number of the particles.

FRET analysis of interactions between actin and exon-exon-junction complex proteins in early mouse embryos.

Spatial interactions between nuclear actin and some proteins of the exon-exon junction complex (EJC) have been demonstrated in the nuclei of early mouse embryos by using Förster resonance energy transfer (FRET). FRET has been registered for the pairs actin-Y14, actin-Aly/REF and actin-NXF1/TAP in nucleoplasmic, irregularly shaped zones and in nucleolus precursor bodies (NPBs). We suggest that FRET signals in the nucleoplasm correspond to the bordering zone of transcriptionally active chromatin.

A regression system for estimation of errors introduced by confocal imaging into gene expression data in situ.

Background: Accuracy of the data extracted from two-dimensional confocal images is limited due to experimental errors that arise in course of confocal scanning. The common way to reduce the noise in images is sequential scanning of the same specimen several times with the subsequent averaging of multiple frames. Attempts to increase the dynamical range of an image by setting too high values of microscope PMT parameters may cause clipping of single frames and introduce errors into the data extracted from the averaged images.

Quantitative investigation of reproduction of gonosomal condensed chromatin during trophoblast cell polyploidization and endoreduplication in the East-European field vole Microtus rossiaemeridionalis.

Simultaneous determinations of DNA content in cell nuclei and condensed chromatin bodies formed by heterochromatized regions of sex chromosomes (gonosomal chromatin bodies, GCB) have been performed in two trophoblast cell populations of the East-European field vole Microtus rossiaemeridionalis: in the proliferative population of trophoblast cells of the junctional zone of placenta and in the secondary giant trophoblast cells. One or two GCBs have been observed in trophoblast cell nuclei of all embryos studied (perhaps both male and female). In the proliferative trophoblast cell population characterized by low ploidy levels (2-16c) and in the highly polyploid population of secondary giant trophoblast cells (32-256c) the total DNA content in GCB increased proportionally to the ploidy level.