Taxonomic and metabolic diversity of Actinomycetota isolated from faeces of a 28,000-year-old mammoth.

Ancient environmental samples, including permafrost soils and frozen animal remains, represent an archive with microbial communities that have barely been explored. This yet unexplored microbial world is a genetic resource that may provide us with new evolutionary insights into recent genomic changes, as well as novel metabolic pathways and chemistry. Here, we describe Actinomycetota Micromonospora, Oerskovia, Saccharopolyspora, Sanguibacter and Streptomyces species were successfully revived and their genome sequences resolved.

HDAC6 detector: online application for evaluating compounds as potential histone deacetylase 6 inhibitors.

The HDAC6 (histone deacetylase 6) enzyme plays a key role in many biological processes, including cell division, apoptosis, and immune response. To date, HDAC6 inhibitors are being developed as effective drugs for the treatment of various diseases. In this work, adequate QSAR models of HDAC6 inhibitors are proposed.

HDAC1 PREDICTOR: a simple and transparent application for virtual screening of histone deacetylase 1 inhibitors.

Histone deacetylases play an important role in regulating gene expression by modifying histones and changing chromatin conformation. HDAC dysregulation is involved in many diseases, such as cancer, autoimmune and neurodegenerative diseases. Histone deacetylase 1 (HDAC1) inhibitors represent an important class of drugs.

Structural fractal analysis of the active sites of acetylcholinesterase from various organisms.

Acetylcholinesterase (AChE) is the object of many studies due to the fact that it plays an important role in the vital activity of organisms. In particular, when new AChE inhibitors are developed, much attention is paid to the specificity of their action. One of the approaches used to study the specificity is to compare AChE taken from various organisms.

QSAR analysis and experimental evaluation of new quinazoline-containing hydroxamic acids as histone deacetylase 6 inhibitors.

Histone deacetylase inhibitors represent the most important class of drugs for the treatment of human cancer and other diseases due to their influence on cell growth, differentiation, and apoptosis. Among the well-known eighteen histone deacetylases, histone deacetylase 6 (HDAC6), which is involved in oncogenesis, cell survival, and cancer cell metastasis, is of great importance. Using the CDK and alvaDesc molecular descriptors and the Random Forest and EXtreme Gradient Boosting methods, we propose a number of adequate QSAR classification models, which are integrated into a consensus model and are freely available on the OCHEM web platform (https://ochem.

Structural Fractal Analysis of the Active Site of Acetylcholinesterase in Complexes with Huperzine A, Galantamine, and Donepezil.

The fractal dimension (D) of the active site of hAChE in the unliganded state and as part of complexes with hyperzine A, galantamine, and donepezil is calculated using molecular interatomic-distance histograms. Fractal matrices of structural changes (FMSCs) are formed by pairwise comparison of the values of D and by revealing the significance of their differences. FMSCs are found to be used to quantitatively estimate the changes in the structures of the molecules in various states.

QSAR analysis of the acute toxicity of avermectins towards .

Avermectins have been effectively used in medicine, veterinary medicine, and agriculture as antiparasitic agents for many years. However, there are still no reliable data on the main ecotoxicological characteristics of most individual avermectins. Although many QSAR models have been proposed to describe the acute toxicity of organic compounds towards (), avermectins are outside the applicability domain of these models.

Stable Bicyclic Functionalized Nitroxides: The Synthesis of Derivatives of Aza-nortropinone-5-Methyl-3-oxo-6,8-diazabicyclo[3.2.1]-6-octene 8-oxyls.

In recent decades, bicyclic nitroxyl radicals have caught chemists' attention as selective catalysts for the oxidation of alcohols and amines and as additives and mediators in directed C-H oxidative transformations. In this regard, the design and development of synthetic approaches to new functional bicyclic nitroxides is a relevant and important issue. It has been reported that imidazo[1,2-]isoxazoles formed during the condensation of acetylacetone with 2-hydroxyaminooximes having a secondary hydroxyamino group are recyclized under mild basic catalyzed conditions to 8-hydroxy-5-methyl-3-oxo-6,8-diazabicyclo[3.

Memory Effect in the Spatial Series Based on Diamond and Graphite Crystals.

To study the relation between the structure of a compound and its properties is one of the fundamental trends in chemistry and materials science. A classic example is the well-known influence of the structures of diamond and graphite on their physicochemical properties, in particular, hardness. However, some other properties of these allotropic modifications of carbon, e.

The Influence of Structural Patterns on Acute Aquatic Toxicity of Organic Compounds.

Investigation of the influence of molecular structure of different organic compounds on acute toxicity towards Fathead minnow, Daphnia magna, and Tetrahymena pyriformis has been carried out using 2D simplex representation of molecular structure and two modelling methods: Random Forest (RF) and Gradient Boosting Machine (GBM). Suitable QSAR (Quantitative Structure - Activity Relationships) models were obtained. The study was focused on QSAR models interpretation.

Complete mitochondrial genome of a woolly mammoth () from Maly Lyakhovsky Island (New Siberian Islands, Russia) and its phylogenetic assessment.

We present a complete sequence and an annotation of the mitochondrial genome of the woolly mammoth () found in 2012 on Maly Lyakhovsky Island (North-Eastern Siberia, Russia). The genome was 16,851 bp long and contained 13 protein-coding, 22 tRNA, and 2 rRNA genes. It was AT reach (61.

Design, Synthesis, and Cancer Cell Growth Inhibitory Activity of Triphenylphosphonium Derivatives of the Triterpenoid Betulin.

A series of new triphenylphosphonium (TPP) derivatives of the triterpenoid betulin (1, 3-lup-20(29)-ene-3β,28-diol) have been synthesized and evaluated for cytotoxic effects against human breast cancer (MCF-7), prostate adenocarcinoma (PC-3), vinblastine-resistant human breast cancer (MCF-7/Vinb), and human skin fibroblast (HSF) cells. The TPP moiety was applied as a carrier group through the acyl linker at the 28- or 3- and 28-positions of betulin to promote cellular and mitochondrial accumulation of the resultant compounds. A structure-activity relationship study has revealed the essential role of the TPP group in the biological properties of the betulin derivatives produced.

[Studies of the biological age in adult taiga ticks Ixodes persulcatus (Ixodinae)].

The history of studies of the biological age in ixodid ticks is discussed. A method of estimation of the biological age in adult ticks of the genus Ixodes by the degree of fat inclusions in midgut cells and in the fat body is developed. An "age scale" for the determination of the calendar age was assumed.

[The connection of polymorphism I/D of the gene angiotensin-converting ferment and D442G gene of protein-carrier of cholesterol ethers with atherosclerosis risk factors in patients of elderly and senile age with coronary heart disease in the Republic Sakha (Yakutia)].

The authors analyse allocation of genotypes frequencies of polymorphism I/D of the gene of angiotensin-converting ferment (ASYA) and D442G gene of protein-transmitting agent of cholesterol ethers (CETP) in patients of elderly, senile age and long-livers with coronary heart disease taking into account their nationality, age and sex. With the age, the frequency reduction of occurrence of genotype ACE*I/*I has been observed and there is a tendency to increase of genotype frequency ACE*D/*D. Sexual distinctions in frequency of revealing of homozygous genotype ACE*D/*D have been revealed at the relative analysis of genotypes ACE*D/*D and ACE*D/*I.

[Age changes in Ixodes pavlovskyi (Ixodinae) natural populations from Novosibirsk and its outskirts].

The age structure of I. pavlovskyi natural populations from Novosibirsk and its outskirts was studied with the use of the fat reserves in the midgut and in the fat body as the age index.

[Estimation of the biological age in males of the taiga tick (Ixodes persulcatus: Ixodinae) by fat reserves in the midgut].

Some criteria for the estimation of the biological and calendar age by the fat storage in midgut cells of Ixodes persulcatus males were established on the basis of examination of ticks from the laboratory culture.

[Survival rate of the geriatric patients of native and non-native nationality with ischemic heart disease in Sakha Republic (Yakutia): analysis of 5-year catamnesis observation].

222 geriatric patients of native and non-native nationality with ischemic heart disease were supervised during 5 years. The analysis of survival rate (causes of death) has shown that 46.4% of patients (n = 103) died within this period of supervision.

[PCR identification of DNA of hosts of the taiga tick nymphs (Ixodes persulcatus: Ixodinae) in St. Petersburg and its suburbs].

PCR identification of host DNA in unfed females and males of taiga tick Ixodes persulcatus was performed. Amplification of each sample was done using primers species-specific by 12S rDNA mitochondrial gene. Four species of small mammals (Apodemus uralensis, Clethrionomys glareolus, Microtus arvalis, and Sorex araneus) and two passeriform bird species (Fringilla coelebs and Parus major) were analysed.

[Ethnic features of the risk factors of development the atherosclerosis and ischemic heart disease at the geriatric contingent of Sakha Republic (Yakutia)].

The study assessed the ethnic, age, gender distinctions on risk factors between elderly, senile age and long-livers male and female of native and non-indigenous nationalities with ischemic heart disease. 354 patients aged from 60 till 106 years comparable on age and gender data were included in the research. There were 2 basic groups considering the ethnic accessory (native and non-indigenous citizens); 3 groups considering the age characteristics (elderly, senile age and long-livers); gender groups -male and female.

[The estimation of risk factors of the atherosclerosis in persons over 60 years of autochthonal and non-indigenous nationalities with the ischemic heart disease old living in the far north].

The study assessed the ethnic, age, gender distinctions on risk factors between elderly, senile age and long-livers male and female of autochthonal and non-indigenous nationalities with ischemic heart disease. 354 patients aged from 60 till 106 years comparable on age and gender data, had been included in research. There were 2 basic groups considering the ethnic implement (autochthonal and nonindigenous citizens); 3 groups considering the age characteristics (elderly, senile age and long-livers); gender groups - male and female.

[Formalin fixation in the method of the Ixodes persulcatus (ixodidae) age estimation by the state of fat reserves].

Formalin fixation is proved to be the useful tool in the method of age estimation for the taiga ticks collected in field. Formalin does not deform fixed material and does not dissolve fat; hence, it is the best fixing fluid when tick age is estimated by the state of fat reserves in its organism. The ticks preserved in 9% formalin were shown to be suitable for age estimation 1.

[Forming radiation load on humans for territory being under influence of radioactive waste release from Nuclear Power Plants].

The results of long-term radioecological researches (1980-2005 years), executed on the south of Ukraine on the study of forming of irradiation dose of man as a result of gaz-aerozol extrass and liquid upcasts of radionuclid's from operating AEPS (South Ukraine and Zaporozhia) in the mode of their normal exploitation are presented in the article. Different ways are considered of stantion radionuclids migration in the external environment. The sizes of effective dose of irradiation of man are certain from the receipt of "station" radionuclid's on the considered migratory chainlets.

[Estimation of the biological age in taiga tick females (Ixodes persulcatus:Ixodidae) by the fat reserves in organism].

The method of estimation of the biological age in non-feeding tick females by the level of adipose inclusions in the cells of the midgut and fat body is developed. In order to estimate the fat reserves in non-feeding females, alive ticks were dissected and fragments of their internal were vitally stained with the pregnant solution of sudan III in 70 % ethanol. Three age-specific groups were established: I, young females whose intestines and fat body were filled with fat inclusions; II, mature females whose fat reserves were partially expended; III, old females having isolated fat inclusions in their midgut and fat body.

[Estimation of the biological age in females of the taiga tick Ixodes persulcatus by changes in the body shape and surface of cuticle].

A method of visual estimation of the biological age of living hungry tick females by visible changes in the depth of marginal groove and the structure of the alloscutum cuticle during natural ageing is developed. In recently activated individuals, the body is convex and the marginal groove is exposed, demonstrating distinctly visible cuticular microfolds (Figs 1-4). In attenuated ticks, the body is flattened and marginal fold overlays the marginal groove, concealing cuticular microfolds (Figs 5-8).