[Composition of the oil-slime microbial community determined by analysis of the 16S rRNA gene].

Analysis of the 16S rRNA genes of the cultured microorganisms of industrial oil-slime revealed predominance (-85-90%) of the Gammaproteobacteria in the community of aerobic heterotrophs and specific oil-slime degraders. Relation of the isolated strains with members of the genera Pseudomonas, Stenotrophomonas, and Enterobacter was established. Analysis of the same gene in the total DNA from the oil-slime revealed greater microbial diversity (-20 operative taxonomic units determined by T-RFLP) than in the cultured part of the community, which included -12 different colony types.

[Influence of reamberin on the photohemotherapy of endogenous intoxication caused by acute experimental pancreatitis].

Combined application of photohemotherapy and antihypoxant reamberin for the treatment of endogenous intoxication favors fast (within one day) restoration of the functional condition of lungs and prevents the development of respiratory distress syndrome. High efficiency of the combined therapy is related to a fast stabilization of membranes, which is determined by the ability to correct lipid metabolism in lung tissues.

[Correlation between the receptor specificity of pandemic influenza A (H1N1)pdm09 virus strains isolated in 2009-2011 and the structure of the receptor-binding site and the probability of fatal primary viral pneumonia].

The receptor specificity (RS) of pandemic influenza A(H1N1) pdm09 virus strains deposited into the State Collection of Viruses of the Russian Federation, D. I. Ivanovsky Research Institute of Virology, Ministry of Health and Social Development of Russia, in the 2009-2010 and 2010-2011 epidemic seasons to a panel of 9 sialoglycopolymers (SGP).

The chromosomes of Tsing-Ling pika, Ochotona huangensis Matschie, 1908 (Lagomorpha, Ochotonidae).

The karyotype of the Tsing-Ling (Huanghe) pika, Ochotona huangensis Matschie, 1908 from the forest habitats of the Qinling Mountains (Shaanxi Province, China) was described for the first time. The chromosome set contains 42 chromosomes (NFa=80). The autosomes are 15 meta-submetacentric pairs and 5 subtelocentric pairs.

[Antioxidant efficacy in the treatment of cardiac metabolism disorders during endotoxicosis].

Chronic experiments on dogs showed that antioxidant drugs (reamberin, ximedone, vitamin E) are highly effective in correcting the functional and metabolic disorders in myocardium under endogenous intoxication conditions. Recovery of the functional activity was accompanied by correction of the lipid metabolism of cardiac cell structures. One mechanism of the drug action is related to a decrease in the lipid peroxidation rate and phospholipase A2 activity in cardiac tissue structures.

[Studying efficacy of remaxol in the treatment of respiratory distress syndrome under endotoxicosis conditions].

Chronic model experiments on dogs showed that the development of respiratory distress syndrome under acute pancreatitis conditions is correlated with violation of metabolic processes in the lung tissue, which is caused by activation of the coagulation/lysis system and changes in the lipid metabolism. It is established that remaxol limits the development of inflammatory processes in lungs, produces correction of the lipid metabolism, and introduces positive changes in the coagulation system lung tissues under acute pancreatitis conditions.

[Correction of endogenous intoxication syndrome in acute pancreatitis].

The endogenous intoxication syndrome was determined with chronic experiments to correlate to functional and metabolic disturbances of liver and intestines under acute pancreatitis. The remaxol use therapy corrects endogenous intoxication syndrome that based on hepato- and enteroprotective medicine effects.

[Cardioprotective and lipid-regulating effects of etoxidol with respect to surgical endotoxinemia].

Chronic experiments on stray dogs with modeling peritonitis showed that a complex therapy involving etoxidol favors improvement of the electrophysiological and metabolic heart activity and provides correction of the lipid distress syndrome. It is established that the lipid regulating effect of etoxidol is related not only to a decrease in the intensity of lipid peroxidation (LPO), but also to the A2-phospholipasae activity of this drug.

Effects of dehydroepiandrosterone sulfate on the conversion of corticosterone into 11-dehydrocorticosterone in stress: a regulatory scheme.

In male rats, repeated but not single exposures to stress increased the conversion of corticosterone (CS) to 11-dehydrocorticosterone (11-DHCS), particularly on the background of administration of dehydroepiandrosterone sulfate (DHEAS). Naltrexone given 20 min before DHEAS at a dose of 0.1 mg/kg, at which it selectively blocks mu opioid receptors, prevented this effect of DHEAS, which is evidence that it is mediated by mu opioid receptors.

[Dynamics organ blood flow in rats in postnatal ontogenesis].

In the 4-, 13-, 30-, and 65- 90-day-old Wistar rats the voluminous blood flow rate was measured in liver, kidney, small intestine wall, and in the predominantly white gracilis muscle of femur. A LAKK-01 laser-Doppler flowmeter and its cutaneous (for kidney) and needle (for other organs) probes were used; they provided estimation of blood flow (per organ mass unit) to the tissue depth of about 1 mm. It has been found that the blood flow rates (per organ mass unit fall in liver, intestine, and even more in muscle, whereas increase in kidney, particularly for the first month of life.

[The denydroepiandrosterone sulfate thyroid activation through the mu-opioid receptors under cold influences].

Denydroepiandrosterone-sulfate (DHEAS) effects on total thyroxine (tT4), total triiodothyronine (tT3), and free thyroxine (tT4) levels were studied in male rats under single or repeated (for 19 days, 1 hr a day) cold influences, as well as participation of mu-opioid receptor effects in DHEAS (30 mg/kg). It was shown that the increased tT4 and tT3 levels under repeated cold influences were more obvious after DHEAS injections. The opioid antagonist naltrexone in the dose 0.

[Relationship of nuclear import of influenza virus nucleoprotein polymers to their conformational status].

It has been earlier shown that in the cells infected with influenza virus, the molecules of nucleoprotein (NP) are polymers that differ in their conformational maturity and stability. The present investigation has studied the ability of different conformational forms of NP polymers to migrate into the nucleus. Conformationally mature compact NP oligomers are shown to predominantly import into the nucleus.

[Role of lipid-modifying components of biomembranes in antipancreatic effect of metabolic drugs].

The high effectiveness of metabolic medication in acute pancreatitis therapy was demonstrated by experimental and clinical researches. The medication capability for knocking-over of systemic lipid distress-syndrome determines its membraneprotective effect, which promotes correction of functional disorders of different organs cell structures and decrease of intoxication syndrome. The expressed lipid corrective effect of metabolic therapy determines major pathogenesis component of its high clinical effectiveness under acute pancreatitis, that confirms important role of lipid modificated component of cell structures membrane by metabolic therapy in knocking-over of acute pancreas inflammation.

[Dehydroepiandrosterone sulfate effect at the conversion of corticosterone to 11-dehydrocorticosterone in stress conditions: the scheme of regulation].

It had been shown that the reaction of conversion of corticosterone (CS) to 11-dehydrocorticosterone (11-DHCS) was increased in chronic intermissions, but not in sharp stress influences in male rats, especially under dehydroepiandrosterone sulfate (DHEAS) employment. The injection of naltrexone 20 min before DHEAS in dose 0.1 mg/kg which selectively blocked the mu-opiate receptors canceled this effect that indicated the participation of in-opiate receptors in such regulation.

[Native and renatured oligomer-dependent epitopes of intracellular influenza virus nucleocapsid protein].

Intracellular NP oligomers have been shown to react with some anti-NP monoclonal antibodies (mAbs) in radio-immnoprecipitation, immunoblotting, and dot immunoassay. Soluble NP monomers obtained after thermal dissociation of NP oligomers are not recognized by mAbs unlike the NP monomers whose concentration increased by about 100-fold due to transfer to the nitrocellulose membrane after polyacrylamide gel electrophoresis. The findings demonstrated that in the intact NP oligomers there were epitopes determined by their quaternary structure.

[Development of encephalitis in modeling of acute hemorrhagic stroke--a new variant of a somatic complication].

We studied immunological and morphological changes in rat brain in an acute period of experimental hemorrhagic stroke (HS). The latter was induced mechanically by the method of A. N.

[The muscular blood flow in rats in ontogenesis as measured by the needle probe laser Doppler flowmeter "LAKK-01"].

In anesthetized Wistar rats of the age of 4, 13, 30 and 90 days, blood flow in m. gracilis was measured with the aid of laser-Doppler flowmeter "LAKK-01" with a needle probe. The sharpest postnatal falling of a muscular blood flow was revealed in the first month of life.

[Two types of NP-NP associations in influenza virus-infected cells].

Two types of NP-NP associations are shown to form in the influenza virus-infected cells. Early NP synthesis gives rise to NP associations stabilized by relatively weak bonds. These structures are designed as NP multimers.

[Effect of Panavir on influenza A virus reproduction].

Mitogenic properties of panavir, as well as its effect on the grippe virus reproduction in cell systems in vitro and the effect on the survival of mice with the experimental grippe infection were studied. It was shown that panavir had no cytotoxic action whereas it was characterized by pronounced mitogenic activity and subsequently could be considered as a perspective immunomodulator. Under in vitro conditions with the use of relatively high doses for the cell contamination with the grippe virus, panavir lowered the virus production in the cell systems.

[Mitotic activity of pial microvascular endothelium rat in postnatal ontogenesis].

Mitotic activity of endothelium in pial microvessels has been studied with the aid of intra-vessel autoradiography in 4-, 12- and 30-day old rats using these parameters: number of labeled nuclei per vessel or per 100 mcm of its length, or per 1 mm2 endothelial surface area, as well as number of vessels with labeled endothelium cells. The first parameter was independent of vessel diameter. The other parameters had highest value in the pericapillary vessels in all rats.

[Temporary acquisition of intra-chain disulphide bonds by nucleocapsid protein of influenza virus].

The in vitro reducing agents were shown to promote the NP-NP association and to stabilize the NP oligomers, which dissociate when heated in non-reducing buffer. This confirms that non-covalent linkages in electrophoresis stabilize the influenza virus NP oligomers. The mobility of pulse-labeled and chased NPs in PAGE as well as their sensitivity to protease were investigated.

[Proliferative activity of blood microvascular endothelium of intestinal mesentery during rat postnatal ontogenesis].

The study of mitotic activity of mesenteric microvascular endothelium cells (EC) in 4-, 12-, and 30-day old rats has been carried out using following parameters: number of labeled nuclei per vessel, or per 100 microm of vascular length, or per 1 mm2 of endothelial surface area, as well as shares of labeled EC and of vessels with labeled EC, have been estimated. The highest density of labeled nuclei was revealed in the pericapillary vessels in all rats. Its values were significantly higher in 12-day-old rats and were the lowest in 30-day-old ones.

[Relaparotomy. Problems of terminology].

The authors discuss the term "relaparotomy" and such its definitions as "early", "delayed", "late", "elective", "targeted", analyze different classifications of relaparotomies, propose their own definition of this term based on objective criteria of general reaction to surgical stress in the form of Seli[symbol: see text] adaptation syndrome which has definite duration (60 days). This fact may be regarded as a clinical criterion of determination of relaparotomy's terms. Surgery may be named as relaparotomy when it is performed in terms of adaptation syndrome (60 days).