[The ARI etiology among children in Belarus in 2011-2012].

The seasonal distribution of the respiratory viruses for the period of 2011-2012 is presented. The ARI etiological structure among children 0-17 years, who were admitted to the hospital for respiratory disease in Belarus, was defined by the PCR-method. It was found that the etiological agents of the infections were not only influenza viruses A and B, parainfluenza types 1-4, adeno- and respiratory syncytial viruses, but also described boca- and metapneumoviruses.

[Suppression of the reproduction of the human immunodeficiency virus (HIV) with a cytokine-like antiviral factor].

Cytokine-like antivirus factor, obtained from the culture fluid of chick embryo fibroblasts, was found to possess inhibitory activity with respect to HIV-1 (strains 1/Zmb and RF). The treatment of HIV-infected cell culture with cytokine-like factor was accompanied by a decrease in the cytopathic effect of the agent without manifestations of cytotoxicity. The combined use of the cytokine-like factor and azidothymidine at near-threshold concentrations was not accompanied by an increase in the antivirus effect, observed after the use of each preparation separately.

[The efficacy of an antiviral factor obtained from infected chick embryo fibroblasts in an experimental herpetic infection].

Treatment of mice with an antiviral factor obtained from the culture medium of infected chick embryo fibroblasts protected them against lethal meningoencephalitis caused by herpes simplex virus. The degree of protection varied from 50 to 75% depending on the virus dose, the route of infection, and the mode of the antiviral factor application.

[Antiviral factor production by infected chick embryo fibroblasts].

The secretion of antiviral factor (AF) by infected cell cultures was examined. Activity of AF depended on the cell culture used. AF produced by infected chick embryo fibroblasts had maximal activity.

[Antiviral factor isolated from infected cell cultures].

An antiviral factor of protein nature inhibiting reproduction of VEE, herpes, vaccinia VSV, fowl plague viruses was isolated from infected cell cultures. The factor has no virucidal or prophylactic effect, stable to low pH values and heating at 100 degrees C for 30 min.

Development of resistance to 2-(1-amino-ethyl)-bicyclo (2.2.1) heptane chlorohydrate in influenza A virus.

A resistant influenza virus has been obtained during successive passages of influenza A virus in 10 to 11-day-old chick embryos (CE) in the presence of 2-(1'-amino-ethyl)-bicyclo(2.2.1) heptane chlorohydrate possessing a high antiviral activity.

[Mechanism of action of 2-(1'-aminoethyl)-bicyclo[2.2.1]heptane hydrochloride].

The effect of 2-[1'-aminoethyl)-bicyclo[2.2.1]heptane chlorohydrate on the accumulation of hemagglutinating activity of chick embryo fibroblasts infected with influenza A/FPV/Rostock/34 (Hav1N1) and A/WSN/33 (H0N1) was studied.

[Activation of the anticoagulant system by prethrombin 2, a thrombin precursor].

Prethrombin 2, and immediate precursor of thrombin, was obtained by limited trypsin or active factor X proteolysis by prethrombin 1. The conversion of prethrombin 1 to prethrombin 2 does not occur in the absence of enzymes. It has been shown that factor V has no influence on the rate of thrombin generation from prethrombin 2 and that prethrombin itself has no coagulant or esterase activity.

Strain-specific degradation of a viral glycoprotein in Newcastle disease virus-infected cells.

In cells infected with mesogenic or lentogenic strain of Newcastle disease virus the level of neuraminidase and hemagglutinin activities sharply decreased after the addition of cycloheximide. With two velogenic strains such decreases did not occur. The infected cells were labelled with 14C-amino acids (leucine or valine) and further incubated with an excess of unlabelled precursor.