Publications by Gregorz Sabat

Publications by authors named "Gregorz Sabat"

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Expression of a translationally fused TAP-tagged plasma membrane proton pump in Arabidopsis thaliana.

Rachel B Rodrigues Gregorz Sabat Benjamin B Minkoff Heather L Burch Thao T Nguyen

Biochemistry

January 2014

The Arabidopsis thaliana plasma membrane proton ATPase genes, AHA1 and AHA2, are the two most highly expressed isoforms of an 11 gene family and are collectively essential for embryo development. We report the translational fusion of a tandem affinity-purification tag to the 5' end of the AHA1 open reading frame in a genomic clone. Stable expression of TAP-tagged AHA1 in Arabidopsis rescues the embryonic lethal phenotype of endogenous double aha1/aha2 knockdowns.

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