Potential for largemouth bass virus to associate with and gain protection from bacterial biofilms.

Quantitative PCR (qPCR) was used to investigate whether largemouth bass virus (LMBV) can exist within biofilms. Suspended LMBV was partitioned into either laboratory-grown Pseudomonas fluorescens biofilms or pond-grown, mixed-population biofilms. Biofilm-entrapped LMBV retained infectivity when tested on epithelioma papillosum cyprini tissue culture cells.

Largemouth bass virus in Texas: distribution and management issues.

In response to fish kills at prominent fishing sites for largemouth bass Micropterus salmoides, such as Lake Fork and Sam Rayburn Reservoir, the Texas Parks and Wildlife Department began a systematic evaluation of state waters for the presence of largemouth bass virus (LMBV). The survey comprised 49 water bodies and 13 river basins, and a total of 2,876 adult bass were collected by electrofishing and angling during the summer and fall of 2000. The virus was initially detected by means of cell culture and its presence subsequently confirmed by polymerase chain reaction.