Publications by authors named "Gregory A Shanower"
The majority of the heme-binding proteins possess a "heme-pocket" that stably binds to heme. Usually known as housekeeping heme-proteins, they participate in a variety of metabolic reactions (e.g.
View Article and Find Full Text PDFAn assessment of histone-modification antibody quality.
Nat Struct Mol Biol
January 2011
Article Synopsis
- The study evaluated over 200 antibodies targeting 57 histone modifications in fruit flies, worms, and human cells.
- While many antibodies worked effectively, over 25% did not pass specificity tests, and among the specific ones, more than 20% failed in further experimental applications.
- The authors recommend careful testing of these antibodies before use and created a website for sharing new test results.
We have identified a novel gene named grappa (gpp) that is the Drosophila ortholog of the Saccharomyces cerevisiae gene Dot1, a histone methyltransferase that modifies the lysine (K)79 residue of histone H3. gpp is an essential gene identified in a genetic screen for dominant suppressors of pairing-dependent silencing, a Polycomb-group (Pc-G)-mediated silencing mechanism necessary for the maintenance phase of Bithorax complex (BX-C) expression. Surprisingly, gpp mutants not only exhibit Pc-G phenotypes, but also display phenotypes characteristic of trithorax-group mutants.
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