Robust discrimination between closely related species of salmon based on DNA fragments.

Closely related species of Salmonidae, including Pacific and Atlantic salmon, can be distinguished from one another based on nucleotide sequences from the cytochrome c oxidase sub-unit 1 mitochondrial gene (COI), using ensembles of fragments aligned to genetic barcodes that serve as digital proxies for the relevant species. This is accomplished by exploiting both the nucleotide sequences and their quality scores recorded in a FASTQ file obtained via Next Generation (NextGen) Sequencing of mitochondrial DNA extracted from Coho salmon caught with hook and line in the Gulf of Alaska. The alignment is done using MUSCLE (Muscle 5.

Views of research ethics committee members on end-of-participation communications for trial participants who stop taking part: a cross-sectional survey study.

Background: Giving information to trial participants who stop taking part could support them through what can be a difficult process. We previously developed guidance around the ethical acceptability of such information provision, and about how trialists can develop suitable communication materials. There is limited evidence about what research ethics committees think of this issue, and limited guidance about what level of oversight they should have over the proposed communications, or post-consent participant communications generally.

Implementation of decarbonisation actions in general practice: a systematic review and narrative synthesis protocol.

Introduction: There is growing recognition of the importance of primary care in addressing climate change. The World Organisation of Family Doctors has urged general practitioners worldwide to commit to tackling climate change and to serve as agents of systemic and individual change. Though an increasing number of resources have become available to support the decarbonisation of primary care, there remains a lack of evidence about how primary care teams are using them, their reach across practices, their level of adoption and maintenance, their cost impact and their effect on carbon emissions.

Justice, Equity, Diversity, Inclusion, and Belonging: A Health Humanities Consortium Initiative.

The Justice, Equity, Diversity, Inclusion, and Belonging (JEDIB) committee formed in 2022 in order to support diversity and inclusion in the Health Humanities Consortium and to advance best practices for equity and inclusion in the field of medical and health humanities. This Forum Essay describes our first year of work, including participant-led commitment statement crafting and strategic planning. Health humanities-specific JEDIB work is described in detail in essays about disability justice; gender, sex, sexuality, and reproductive justice; and Indigeneity from a decolonial standpoint.

How can we support research participants who stop taking part? Communications guidance developed through public-researcher collaboration.

Background: Research study participants can stop taking part early, in various circumstances. Sometimes this experience can be stressful. Providing participants with the information they want or need when they stop could improve participants' experiences, and may benefit individual studies' objectives and research in general.

Altered grey matter structural covariance in chronic moderate-severe traumatic brain injury.

Traumatic brain injury (TBI) alters brain network connectivity. Structural covariance networks (SCNs) reflect morphological covariation between brain regions. SCNs may elucidate how altered brain network topology in TBI influences long-term outcomes.

The Impact of Hemispheric Activity Priming on Choking Under Pressure in Badminton Tasks: A Study of Three Fundamental Skills.

Choking under pressure occurs when an individual experiences a decrease in performance despite their efforts to perform well. The self-focus approach suggests that pressure increases conscious attention on the performance process, disrupting the automatic or overlearned nature of execution. Hemispheric asymmetries in the brain and skilled performance indicate that left-hemispheric activity decreases, while right-hemispheric activity enhances.

Senior nurses' expectations and support of new graduate nurses' adjustment in hospitals: A qualitative descriptive study.

Aim: This study aimed to identify senior nurses' expectations and support of new graduate nurses' adjustment to their work environment.

Background: New graduate nurses have difficulties in adjusting to their work environment because they are not well prepared for clinical nursing practice upon graduation. Hospitals support their adjustment to their work environment through residency/transition programs.

Challenges faced by nurses engaged in rheumatology care in Japan.

Objectives: This study aimed to identify the challenges faced by nurses engaged in rheumatology care in Japan.

Methods: We conducted a questionnaire survey of nurses in Japan in 2020 on challenges experienced in rheumatology care. The participants were nurses certified by the Japan Rheumatism Foundation.

Dynamic vapor microextraction of ignitable liquid from casework containers.

Dynamic vapor microextraction (DVME) is a headspace concentration method that can be used to collect ignitable liquid (IL) from fire debris onto chilled adsorbent capillaries. Unlike passive headspace concentration onto activated carbon strips (ACSs) that must be eluted with a toxic solvent (carbon disulfide), DVME employs a relatively benign solvent (acetone) to recover the adsorbed IL residue, and each headspace collection is monitored for breakthrough. Here, for the first time, we extend DVME to casework containers while exploring a realistic range of oven temperatures and collection volumes.

Children's Hospital Transfers From Referring Emergency Departments: Which Patients Bypassed the Pediatric Emergency Department?

Objectives: Children are increasingly transferred from emergency departments (EDs) to children's hospitals for inpatient care. The existing literature on the use of direct admission (DA) specifically among pediatric patients transferred from referring EDs remains sparse.The objective of this study was to identify demographic, clinical, and contextual factors associated with the use of direct-to-inpatient versus ED-to-inpatient admission among patients transferred to children's hospitals from EDs.

Convergent validity and sex invariant factor structure of the Movement Imagery Questionnaire-3 - Second version (MIQ-3S): Healthy, young adult reference data.

Aligned with the approach that established the factor structure of the Movement Imagery Questionnaire-3 (MIQ-3), this study extended the two-factor structure of the Movement Imagery Questionnaire - Revised Second version (MIQ-RS). The extension involves assessment of both internal and external visual imagery abilities along with kinesthetic imagery ability. Participants (N = 396) completed the new Movement Imagery Questionnaire - 3 Second Version (MIQ-3S) along with the Vividness of Movement Imagery Questionnaire-2 (VMIQ-2) which measure the same three imagery abilities.

Generative Adversarial Network Performance in Low-Dimensional Settings.

A generative adversarial network (GAN) is an artifcial neural network with a distinctive training architecture, designed to create examples that faithfully reproduce a target distribution. GANs have recently had particular success in applications involving high-dimensional distributions in areas such as image processing. Little work has been reported for low dimensions, where properties of GANs may be better identifed and understood.

Executive functions in agenesis of the corpus callosum: Working memory and sustained attention in the BTBR inbred mouse strain.

Introduction: Agenesis of the corpus callosum (AgCC) is characterized by the congenital partial or complete absence of the corpus callosum. Several strains of mice have been reported to carry AgCC, with the BTBR T Itpr3 /J (BTBR) inbred mouse strain consistently showing a complete absence of the corpus callosum, as well as a variable reduction in the size of the hippocampal commissure. While much research has focused on the social deficits of the BTBR strain, little research on its cognitive behavior has been conducted.

Education Students' Stigma Toward Mental Health Problems: A Cross-Cultural Comparison.

One of the main obstacles to integrating individuals with severe mental disorders into society today is the stigma directed at them. Although breakthroughs in treatment have been made in recent years, many professionals continue to admit that they do not possess enough training to combat this problem. Considering this situation, the present study analyzes the existing stigma among University Education students in three countries with different education systems and cultures, namely Spain, Russia, and Canada.

Variance estimation in tests of clustered categorical data with informative cluster size.

In the analysis of clustered data, inverse cluster size weighting has been shown to be resistant to the potentially biasing effects of informative cluster size, where the number of observations within a cluster is associated with the outcome variable of interest. The method of inverse cluster size reweighting has been implemented to establish clustered data analogues of common tests for independent data, but the method has yet to be extended to tests of categorical data. Many variance estimators have been implemented across established cluster-weighted tests, but potential effects of differing methods on test performance has not previously been explored.

Evidence for the Use of Triage, Respiratory Isolation, and Effective Treatment to Reduce the Transmission of Mycobacterium Tuberculosis in Healthcare Settings: A Systematic Review.

Evidence is limited for infection prevention and control (IPC) measures reducing Mycobacterium tuberculosis (MTB) transmission in health facilities. This systematic review, 1 of 7 commissioned by the World Health Organization to inform the 2019 update of global tuberculosis (TB) IPC guidelines, asked: do triage and/or isolation and/or effective treatment of TB disease reduce MTB transmission in healthcare settings? Of 25 included articles, 19 reported latent TB infection (LTBI) incidence in healthcare workers (HCWs; absolute risk reductions 1%-21%); 5 reported TB disease incidence in HCWs (no/slight [high TB burden] or moderate [low burden] reduction) and 2 in human immunodeficiency virus-positive in-patients (6%-29% reduction). In total, 23/25 studies implemented multiple IPC measures; effects of individual measures could not be disaggregated.

Injury characteristics of the Pulse Nightclub shooting: Lessons for mass casualty incident preparation.

Background: On the morning of June 12, 2016, an armed assailant entered the Pulse Nightclub in Orlando, Florida, and initiated an assault that killed 49 people and injured 53. The regional Level I trauma center and two community hospitals responded to this mass casualty incident. A detailed analysis was performed to guide hospitals who strive to prepare for future similar events.

CArtesian sampling with Variable density and Adjustable temporal resolution (CAVA).

Purpose: To develop a variable density Cartesian sampling method that allows retrospective adjustment of temporal resolution for dynamic MRI applications and to validate it in real-time phase contrast MRI (PC-MRI).

Theory And Methods: The proposed method, called CArtesian sampling with Variable density and Adjustable temporal resolution (CAVA), begins by producing a sequence of phase encoding indices based on the golden ratio increment. Then, variable density is introduced by nonlinear stretching of the indices.

Analysis of the Usefulness of a Serious Game to Raise Awareness about Mental Health Problems in a Sample of High School and University Students: Relationship with Familiarity and Time Spent Playing Video Games.

Background: One of the main challenges in the field of mental health today is the stigma towards individuals who have psychological disorders.

Aims: This study aims to analyse the usefulness of applying a serious game developed for the purpose of raising awareness among students about mental health problems and analyse whether its usefulness can be influenced by the type of video games or the time that students usually devote to playing with this type of entertainment.

Method: The serious game introduces four characters who display the symptoms of different psychological disorders.