Development of an immunochemical detection method for atrazine-induced albumin adducts.

Atrazine (2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine) is one of the most commonly used herbicides in the United States. Exposures in rodent models have led to a host of biological effects, most notably the suppression of luteinizing hormone surge. Previously, we have reported that diaminochlorotriazine (DACT), an atrazine metabolite, forms a covalent adduct with rat hemoglobin at Cys-125.

Identification of a novel hemoglobin adduct in Sprague Dawley rats exposed to atrazine.

Atrazine (2-chloro-4-[ethylamino]-6-[isopropylamino]-1,3,5-triazine) is one of the most commonly used herbicides in North America and is frequently detected in ground and surface waters. This research investigated possible covalent modifications of hemoglobin following in vivo exposures to atrazine in Sprague Dawley (SD) rats and in vitro incubations with diaminochlorotriazine. SD rats were exposed to 0, 10, 30, 100, and 300 (mg atrazine/kg)/day for 3 days via oral gavages, and blood was drawn at 0 h, 24 h, 72 h, 20 days, 1 month, and 2 months for globin analysis.