Feline parasites and the emergence of feline lungworm in the Portland metropolitan area, Oregon, USA 2016-2017.

Objectives: The aim of this study was to determine the prevalence of internal parasites in feral and free-roaming owned cats in the region of Portland, Oregon, USA.

Methods: Fecal samples from asymptomatic cats were opportunistically collected from feral cats presented for surgical sterilization (n = 46), as well as free-roaming owned cats (n = 86) presented to primary care clinics. Fecal analysis was performed using the Baermann technique, centrifugal flotation, fluorescent auramine and fluorescent antibody for species.

Small animal general practice veterinarians' use and perceptions of synchronous video-based telemedicine in North America during the COVID-19 pandemic.

Objective: To assess small animal general practice veterinarians' use and perceptions of synchronous video-based telemedicine before and during the coronavirus disease 2019 (COVID-19) pandemic.

Sample: 550 respondent veterinarian members of the Veterinary Information Network (VIN).

Procedures: An anonymous online survey was used to gather data from VIN-member veterinarians in small animal general practice regarding their perceptions and use of synchronous video-based telemedicine.

Detection of parasites in canine feces at three off-leash dog parks in Portland, Oregon 2014.

Gastrointestinal parasitism is common in dogs attending parks, with variable prevalences of parasites based on geographical region and demographic factors. In this study, canine fecal samples were opportunistically collected from three off-leash dog parks in Portland, Oregon and analyzed for parasites. While some similarities with previous research was seen, much higher rates of Giardia (25.

Owner satisfaction with use of videoconferencing for recheck examinations following routine surgical sterilization in dogs.

OBJECTIVE To evaluate owner satisfaction with a home-based, synchronous videoconferencing telemedicine application as an alternative to in-clinic appointments for conducting recheck examinations after surgical sterilization in dogs. DESIGN Randomized controlled clinical trial. ANIMALS 30 client-owned dogs undergoing elective surgical sterilization and postsurgical recheck examination between September 27, 2017, and February 23, 2018.

Antiproliferative quillaic acid and gypsogenin saponins from Saponaria officinalis L. roots.

Nine quillaic acid and five gypsogenin bisdesmosides were isolated from roots of Saponaria officinalis L. (Caryophyllaceae). Seven of the quillaic acid saponins possessed a 3-O-β-D-galactopyranosyl-(1 → 2)-[β-D-xylopyranosyl-(1 → 3)]-β-D-glucuronopyranosyl unit, but differed from each other in oligosaccharide units linked to the C-28 ester.

Antiproliferative activity of Saponaria vaccaria constituents and related compounds.

Total methanolic extracts of Saponaria vaccaria seed derived from several varieties, as well as various purified components obtained through successive chromatographic separations of total extracts were evaluated for their growth inhibitory activity in WiDr (colon), MDA-MB-231 (breast), NCI-417 (lung) and PC-3 (prostate) human cancer cells as well as the non-tumorigenic fibroblast BJ (CRL-2522) cell line using MTT colorimetric assay. Purified bisdesmosidic saponins segetoside H and I were further examined using microscopy and apoptosis assays. Bisdesmosidic saponins exhibited dose-dependent growth inhibitory and selective apoptosis-inducing activity.

Use of digitoxin and digoxin as internal standards in HPLC analysis of triterpene saponin-containing extracts.

Introduction: Saponins are widely distributed complex plant glycosides possessing a variety of structure-dependent bioactivities. Quantitation of individual saponins is difficult due to lack of available standards, mainly as a consequence of purification difficulties. Determination of total saponin content can be problematic, often relying on non-specific methods based on butanol solubility, haemolytic activity or formation of coloured derivatives.

Analysis of bisdesmosidic saponins in Saponaria vaccaria L. by HPLC-PAD-MS: identification of new quillaic acid and gypsogenin 3-O-trisaccharides.

A high-performance liquid chromatographic method using photodiode array and single quadrupole electrospray mass detection for analysis and profiling of bisdesmosidic saponins in Saponaria vaccaria seed was developed. Profiles of seed extract from three different plant sources were obtained and found to contain the same saponins, albeit in different proportions. Several known saponins were identified by selected ion extraction of quasi-molecular ions from the total ion chromatogram and confirmed by their mass spectra.