A pilot training program for point-of-care ultrasound in Kenya.

Introduction: Ultrasound is an effective and affordable clinical diagnostic tool highly attractive for use in low and middle income countries (LMICs), but access to training programs in these countries is limited. The objective of our study was to develop and pilot a program for healthcare providers in Kenya in the use of point-of-care ultrasound.

Methods: Trainees were recruited in district hospitals for participation in three all-day workshops held every 3-5 months from September 2013 through November 2014.

Outbreak-related Hendra virus infection in a NSW pet dog.

Hendra virus (HeV) infection is a zoonosis of importance in Australia. An outbreak of HeV occurred on the mid-north coast region of New South Wales (NSW) in June and July 2013. Four unvaccinated horses on four separate properties were confirmed to have HeV infection.

A field study of household attack rates and the effectiveness of macrolide antibiotics in reducing household transmission of pertussis.

Bordetella pertussis (whooping cough) is an endemic, highly contagious bacterial respiratory infection, which is notifiable to Australian state and territory health departments. Between 2008 and 2011 there was a substantial outbreak in New South Wales with an initial increase in cases occurring in North Coast New South Wales from late 2007. During September and October 2011 the North Coast Public Health Unit conducted a household study of secondary attack rates to assess the effectiveness of pertussis vaccination as well as the timely use of antibiotics in preventing household transmission.

Development and characterization of genomic SSR markers in Cynodon transvaalensis Burtt-Davy.

Simple sequence repeat (SSR) markers are a major molecular tool for genetic and genomic research that have been extensively developed and used in major crops. However, few are available in African bermudagrass (Cynodon transvaalensis Burtt-Davy), an economically important warm-season turfgrass species. African bermudagrass is mainly used for hybridizations with common bermudagrass [C.

Loss of genetic accuracy in mutants of the thermoacidophile Sulfolobus acidocaldarius.

To investigate how hyperthermophilic archaea can propagate their genomes accurately, we isolated Sulfolobus acidocaldarius mutants exhibiting abnormally high rates of spontaneous mutation. Our isolation strategy involved enrichment for mutator lineages via alternating selections, followed by screening for the production of spontaneous, 5-fluoro-orotate-resistant mutants in micro-colonies. Several candidates were evaluated and found to have high frequencies of pyrE and pyrF mutation and reversion.