Biology of recurrent cholesteatoma in a Romanian young patient - a case report.

Cholesteatoma is an otologic pathology that can occur at any age and can lead to a variety of complications including facial palsy, intracranial abscess, hearing loss, venous thrombosis. Cholesteatoma, even if considered a benign condition, associates high risks of recurrency due to its invasiveness. We describe a case of recurrent cholesteatoma in a young boy who presented chronic ear discharge and hearing loss for which had undergone three surgical interventions between the ages of 16 and 19 years old, from 2019 to 2022.

Clinicopathological correlations and prognostic insights in osteosarcoma: a retrospective analysis.

Osteosarcoma (OS) is the most prevalent primary bone malignancy, predominantly affecting adolescents and young adults, and presents significant clinical challenges due to its aggressive nature and high potential for metastasis. This retrospective study analyzed 34 cases of primary OS, aged 10 to 65 years, to identify clinicopathological correlations that could inform future research and treatment strategies. The findings aim to guide larger cohort studies, essential for validating these correlations and developing tailored approaches that enhance patient outcomes.

Immunoexpression of E-cadherin, β-catenin and Ezrin in non-small cell lung carcinomas.

Lung cancer continues to have the highest mortality rate in the world, non-small cell lung carcinoma (NSCLC) representing the most common tumor form. The therapeutic interference of the tumor intercellular adhesion disruption mechanisms can provide therapeutic targets to improve the patients' prognosis. The study included 52 cases diagnosed with NSCLC, for which the immunohistochemical expressions of E-cadherin, β-catenin and Ezrin were analyzed in relation to the epidemiological and histological prognostic parameters.

Interdisciplinary Research on Medieval Fresco Subjected to Degradation Processes in the Corbii de Piatră Cave Church.

This paper presents research on the degradation processes of the fresco painting in the cave church of Corbii de Piatră Hermitage under the influence of meteoric infiltration water and environmental factors. The medieval fresco dates from the end of the 13th century and the beginning of the 14th century, being painted on a sandstone wall. The infiltration of meteoric water through this wall, the temperature variations, the environment and the repeated wetting/drying processes determined the degradation of the fresco, resulting in its detachment from large surfaces.

Molecular Monitoring of Allogeneic Stem Cell Transplantation in Fanconi Anemia.

Background: Allogeneic hematopoietic stem cell transplantation (HSCT) is the treatment of choice in patients with Fanconi anemia (FA). The aim of our study is to evaluate the impact and benefits of allogenic matched donor HSCT in a case of a 12 year-old girl with FA, who displayed good clinical evolution following 2 months post-transplantation.

Methods: In the pre-transplant phase, reference blood samples from the donor and recipient were collected on EDTA.

Immunoexpression of Ki67, p53 and cyclin D1 in osteosarcomas.

The main malignant tumor of the bone tissue is represented by osteosarcoma, neoplasia with a reserved prognosis and an unpredictable evolution, often aggressive. Cell cycle disruption is one of the complex biomolecular mechanisms involved in the progression of osteosarcomas. In this study, we analyzed the immunoexpression of Ki67, p53 and cyclin D1 for 18 primitive osteosarcomas in relation to the clinicopathological prognosis parameters of the lesions.

About the Experimental and Virtual Analysis of Orthopedic Implant Systems for the Revision of the Hip Prosthesis with Morcellated Bone Graft and Reconstruction Net.

Introduction: Prosthesis loosening is an alteration of the function and position of a total hip prosthesis with reference to the initial surgical moment. The main mechanism unanimously accepted for aseptic prosthetic losses at the level of the cup is represented by the biological mechanism.

Material And Method: Experimental and virtual, interdisciplinary tools, techniques and methods were used to determine the behavior of the hip replacement prosthesis with the morcellated graft and the reconstruction net.

Criterion validity of ultrasound in the identification of calcium pyrophosphate crystal deposits at the knee: an OMERACT ultrasound study.

Objective: To evaluate the discriminatory ability of ultrasound in calcium pyrophosphate deposition disease (CPPD), using microscopic analysis of menisci and knee hyaline cartilage (HC) as reference standard.

Methods: Consecutive patients scheduled for knee replacement surgery, due to osteoarthritis (OA), were enrolled. Each patient underwent ultrasound examination of the menisci and HC of the knee, scoring each site for presence/absence of CPPD.

A Novel Method of Obtaining Platelet Rich Fibrin from Rats and Quantifying Platelet Count.

Background And Objectives: Rat experimentation is the first line of research by which a medical hypothesis is usually tested. Platelet Rich Fibrin (PRF) is a relatively new bio-material that has shown promise to enhance healing in the field of bone research and tissue engineering. In order to perform PRF based experiments on rats, a proper protocol of obtaining PRF from rats needs to be established.

New technical procedure involving Achilles tendon rupture treatment through transcutaneous suture.

The Achilles tendon is the widest tendon of the human body. Achilles tendon belongs to the extrasynovial tendons group and this allows it a faster recovery, thanks to local hematoma from the peritenon, necessary for the scarification. We concluded that in Achilles tendon rupture treatment it is essential to maintain the tendon covering skin integrity, the peritendinous integrity, to maintain the local hematoma formed during and after tendon rupture, reattaching the ruptured tendon heads and maintain them in this position by suturing them and by relaxing the sural triceps muscle.

A chirality change in XPC- and Sfi1-derived peptides affects their affinity for centrin.

The Ca(2+)-binding protein centrin binds to a hydrophobic motif (W(1)xxL(4)xxxL(8)) included in the sequence of several cellular targets: XPC (xeroderma pigmentosum group C protein), Sfi1 (suppressor of fermentation-induced loss of stress resistance protein1), and Sac3 [the central component of the transcription and mRNA export (TREX-2) complex]. However, centrin binding occurs in a reversed orientation (L(8)xxxL(4)xxW(1)) for Sfi1 and Sac3 compared with XPC. Because D-peptides have been investigated for future therapeutic use, we analyzed their centrin-binding properties.

Imaging and histopathological aspects in aseptic osteonecrosis of the femoral head.

Aseptic osteonecrosis causes various clinical manifestations, depending on its location, but has in common a histopathological and radiological substrate. Aseptic osteonecrosis of the femoral head is a condition whose pathogenesis remains unclear despite many theories developed so far, and the discovery of numerous risk factors. The objective of this study is to emphasize the role of imaging techniques and correlating histology and immunohistochemistry methods in order to more accurately stage the disease.

Macrophage response in patients diagnosed with aseptic necrosis of the femoral head presenting different risk factors.

Aseptic necrosis of the femoral head is a condition caused by partial or total interruption of blood supply to the femoral head. The diminished blood supply causes necrosis of the cellular elements and of the bone marrow, followed by the collapse of the bone structure, events that ultimately lead to the destruction of the bone tissue, the appearance of local pain and loss of function in the affected coxofemoral joint. The importance of this condition is that it mainly affects young adults aged 30-50 years, active from a socio-professional standpoint, and increased life expectancy.

A Case of Bilateral Aseptic Necrosis of the Femoral Head.

Aseptic necrosis of the femoral head is a disease whose etiology is not completely elucidated and generally affects young adults aged between 30 and 50 years. In a significant number of patients bilateral disease occurs, which makes detection in its early stages constitute an important objective. We present the case of a male patient, aged 23 years, with the following risk factors: smoking and chronic alcohol consumption, who is diagnosed with aseptic necrosis of the left femoral head, ARCO stage IV, and in just six months after the diagnosis and hip arthroplasty, he suffers an injury which leads to the same diagnosis in the contralateral hip.

Quality assurance in the laboratory testing process: indirect estimation of the reference intervals for platelet parameters in neonates.

Objectives: One of the most powerful tools that laboratory medicine uses to help the clinical decision-making process is the reference interval (RI). By establishing their own RIs in accordance with the analytical method they apply, the laboratories contribute to the quality assurance in the laboratory testing process.

Design And Methods: This study estimates the RIs of the platelet parameters in neonates for Nihon Celltac F hematology automatic analyzer.

CK2 phosphorylation of human centrins 1 and 2 regulates their binding to the DNA repair protein XPC, the centrosomal protein Sfi1 and the phototransduction protein transducin β.

Centrins are calcium-binding proteins that can interact with several cellular targets (Sfi1, XPC, Sac3 and transducin β) through the same hydrophobic triad. However, two different orientations of the centrin-binding motif have been observed: W(1)xxL(4)xxxL(8) for XPC (xeroderma pigmentosum group C protein) and the opposite orientation L(8)xxxL(4)xxW(1) for Sfi1 (suppressor of fermentation-induced loss of stress resistance protein 1), Sac3 and transducin β. Centrins are also phosphorylated by several protein kinases, among which is CK2.

Quality indicators in the preanalytical phase of testing in a stat laboratory.

Objective: To quantify performance in the preanalytical phase in a stat laboratory using quality indicators, and compare our results with those in the literature to improve laboratory services.

Methods: We counted the test request forms, samples, and the types of preanalytical errors that occured in a stat laboratory between January 1 and December 31, 2011. We then compared the quality-indicator scores with the quality specifications mentioned in the literature.

The E144 residue of Scherffelia dubia centrin discriminates between the DNA repair protein XPC and the centrosomal protein Sfi1.

Centrins are members of the EF-hand family of calcium-binding proteins, which are highly conserved among eukaryotes. Centrins bind to several cellular targets, through a hydrophobic triad. However, the W(1)xxL(4)xxxL(8) triad in XPC (Xeroderma Pigmentosum Group C protein) is found in the reverse orientation, as in the L(8)xxxL(4)xxW(1) triad in Sfi1 (Suppressor of Fermentation-Induced loss of stress resistance protein 1).

Quality in post-analytical phase: indirect reference intervals for erythrocyte parameters of neonates.

Objectives: This study aims to gain an estimation of the reference intervals for erythrocyte parameters of neonates in the venous blood, in their first day of life, aiming to achieve quality assurance in post-analytical phase according to the requirement of the ISO standard 15189:2007: Medical laboratories-particular requirements for quality and competence.

Design And Methods: The study was performed on a group of 845 subjects selected from the laboratory database. The blood test was performed with the Nihon Celltac F automatic hematology analyzer.

Macroscopic and microscopic findings in avascular necrosis of the femoral head.

Unlabelled: The avascular necrosis of the femoral head is an illness induced by the cutoff of blood flow to the femoral head and it affects mostly young adults between the ages of 30 and 50 years, raising therapeutic and diagnostic issues. Many risk factors are incriminated in the development of avascular necrosis of the femoral head like: trauma, chronic alcohol consumption, smoking, administration of corticosteroid drugs, most of the cases are considered to be idiopathic. The main goal of our paper is to describe the macroscopic and microscopic variations of the bone structure, which occur in patients with avascular necrosis of the femoral head.

Power Doppler sonography, a non-invasive method of assessment of the synovial inflammation in patients with early rheumatoid arthritis.

Objective: To detect synovitis is important in both the diagnosis and outcome assessment of early rheumatoid arthritis. This study was meant to assess the validity and reproducibility of ultrasonography (US) as a mean of detection for the knee synovitis, by comparing US findings with clinical examination and histopathological findings in synovial membrane.

Methods: The study group included 65 patients with early rheumatoid arthritis - below 12 months from the debut, naive for DMARDs, in whom demographic data - gender, age, disease duration, the number of tender and swollen joints, HAQ score (Health Assessment Questionnaire) and serum samples for CRP, RF, anti-CCP2 antibodies (ELISA, QUANTA LiteTM, CCP IgG, INOVA Diagnostics Inc, USA), VEGF (ELISA, VEGF2, DRG International, IRC, USA) determination were recorded.

The arthroscopy-histological criterion link in the result's estimation of the endoscopic treatment by resection of the knee's meniscus lesions.

The authors present the results of a statistical, clinical, imaging X-ray and optical microscopic studies of some lesions of knee's meniscus, the arthroscopy allowing this structure's biopsy during the endoscopic procedures of resection. These histological criterions are very important estimation factors of long-term results of these techniques, often the microstructure looking different to the appearing normal macroscopic aspect.

A multidisciplinary approach in patients with femoral neck fracture on an osteoporotic basis.

Introduction: Osteoporosis is a common affection characterized by a reduction of bone mass that affects mostly women after menopause. It currently leads to fractures, especially of the spine and hip thus enhancing the costs of medical care. There are many factors that contribute to its development, leading to various strategy lines to deal with it.

Numerical simulations of the 3D virtual model of the human hip joint, using finite element method.

In this paper, we present a three-dimensional mathematical model for a normal hip joint. The three-dimensional finite element model has been constructed based on Computer Tomograph scans of the bones. The obtained 3D model is studied using the finite element method, taking into consideration the real structure of the bone and the mechanical characteristics of cortical and spongy.