Fucoxanthin Enhances the Antifibrotic Potential of Placenta-derived Mesenchymal Stem Cells in a CCl4-induced Mouse Model of Liver.

Background: The effectiveness of fucoxanthin (Fx) in liver diseases has been reported due to its anti-inflammatory and antifibrotic effects. Mesenchymal stem cells (MSCs)-based therapy has also been proposed as a promising strategy for liver fibrosis treatment. Recent studies have shown that the co-administration of MSCs and drugs demonstrates a pronounced effect on liver fibrosis.

Fucoxanthin exert dose-dependent antifibrotic and anti-inflammatory effects on CCl-induced liver fibrosis.

The lack of an effective non-surgical liver fibrosis treatment is a major problem in hepatology. Fucoxanthin is a marine xanthophyll that exhibits anti-inflammatory, antioxidant, and hepatoprotective properties, thereby indicating its potential effectiveness in the treatment of liver fibrosis. The study aims to investigate the antifibrotic and anti-inflammatory effects of fucoxanthin and its main mechanisms on carbon tetrachloride (CCl)-induced liver fibrosis in 50 outbred ICR/CD1 mice.

[Homing multipotent mesenchymal stromal cells in different ways of introduction of old laboratory animals after liver resection.].

The aim of this research was to study the migration of multipotent mesenchymal stromal cells (MMSC) in old laboratory animals under physiological conditions and after liver resection. Different routes of administration were used: to the caudal vein, intraperitoneal, hepatic artery, portal vein. Studies have shown the ability of the old organism to respond to changes in the directed migration of MMSCs in the tissues that have undergone the greatest damage, which may be due to the production of connective tissue of the damaged organ chemoattractant.

[The impact of transplantation of multipotent mesenchymal stromal cells after liver resection on blood biochemical parameters in mature and old laboratory animals.].

The aim of this study was to study the effect of multipotent mesenchymal stromal cells (MMSC) transplantation on blood biochemical parameters after liver resection in Mature and old laboratory animals. The conducted studies show the ability of old laboratory animals to respond to MMSC transplantation after liver resection by reducing the severity of hepatocyte cytolysis. A comparative analysis of the biochemical parameters of Mature and old laboratory animals after liver resection should be noted a more pronounced response of mature animals in relation to the ability of the liver to synthesize urea.

Effects of Combined Transplantation of Multipotent Mesenchymal Stromal and Hemopoietic Stem Cells on Regeneration of the Hemopoietic Tissue.

The effect of allogenic combined transplantation of placental multipotent mesenchymal stromal and hemopoietic stem cells on regeneration of the myeloid tissue and spleen after acute blood loss was studied in laboratory mice. Combined transplantation of these cells did not change the content of cytogenetically modified cells in the bone marrow under normal conditions, but reduced their levels after acute blood loss. Combined transplantation of multipotent mesenchymal stromal and hemopoietic stem cells promoted activation of erythropoiesis and granulocytopoiesis.

[CHANGING OF THE MORPHOMETRIC AND CYTOLOGICAL INDEXES OF THE SPLEEN IN THE CONDITION OF ACUTE BLOOD LOSS ON THE BACKGROUND OF STEM CELLS INSERTION].

The objective of the work was to study morphometric and cytological indexes of the spleen in mature and old laboratory animals after acute blood loss on the background of allogenic transplantation of placenta MMSC and GSC. The study proved that 5 days after acute blood loss on the background of stem cells transplantations to the mature animals there was a reduction of lymphoid follicles area due to reduction in thymus independent area. There were no immunosuppressive effects to the white pulp of the spleen in old animals.

[Experimental study of the effect of combined transplantation of multipotent mesenchymal stromal cells and hematopoietic stem cells on the regeneration of the intestinal epithelium in the conditions of influence of extreme factors].

The study examined the effect of combined transplantation of multipotent mesenchymal stromal and hematopoietic stem cells on the regeneration of the intestinal epithelium in the conditions of influence of extreme factors. Obtained in physiological conditions and in the conditions of influence of extreme factors combined transplantation of multipotent mesenchymal stromal and hematopoietic stem cells derived from mature and old animals is accompanied by increased content of epithelial cells of the crypts of the jejunum. In physiological conditions in mature animals, this effect is realized through the increase of proliferative activity of epithelial cells, while the old - through the inhibition of apoptosis.

[The opportunity to use combined stem cells transplantation for haemopoesis activation in the old and mature laboratory animals under the conditions of ionizing radiation].

The objective of this work was to study the influence of combined transplantation of stem cells (multypotent mesenchimal stromal and hem poetic stem cells) on the haemopoesis of old and mature laboratory animals under the condition of ionizing radiation. The experiments were conducted on 48 white male mice with the body weight of 30 g, age of 3-4 months, and 48 male mice of 3 years of age and body mass of 50 g. The experiments for obtaining the MMSC and HSC cultures were conducted on 16 laboratory animals: female mice of 3-4 months of age and body mass of 30 g.

[The opportunity to use combined stem cells transplantation for haemopoesis activation in the old and mature laboratory animals under the conditions of ionizing radiation].

The objective of this work was to study the influence of combined transplantation of stem cells (multypotent mesenchimal stromal and haemopoetic stem cells) on the haemopoesis of old and mature laboratory animals under the condition of ionizing radiation. The result of the experiment shows that under physiological conditions the combined transplantation brings the erithropoesis activation, under the ionizing radiation conditions it brings the erythroid and granulocytopoesis activation. Moreover the combined MMSC and HSC transplantation gives cytoprotective action on the myeloid tissue due to decrease of cyto genically changed cells in the mature animals under the condition of ionizing radiation, but in the old animals this effect can be seen even under physiological condition.

[Treatment of extracranial gunshot wounds caused by non-lethal weapon].

The deaths happened as a result of wounds caused by non-lethal weapon (NLW) are mainly associated with severe and extremely severe damage of brain. However, vast number of specific problems occurs in cases of extracranial gunshot wounds caused by NLW. Taking into account little physicians' experience, and absence of standard approaches to treatment of these wounds till now.