Impact of trainees involvement on surgical outcomes of abdominal and laparoscopic myomectomy.

Background: To determine the association of trainees involvement with surgical outcomes of abdominal and laparoscopic myomectomy including operative time, rate of transfusion, and complications.

Methods: A retrospective cohort study of 1145 patients who underwent an abdominal or laparoscopic myomectomy from 2008-2012 using the American College of Surgeons National Surgical Quality Improvement Program database (Canadian Task Force Classification II-2).

Results: Overall, 64% of myomectomies involved trainees.

Utility of Routine Preoperative Laboratory Testing for Low-risk Patients in Ambulatory Gynecologic Surgery.

Study Objectives: To examine the current use of routine preoperative laboratory testing in low-risk patients undergoing ambulatory gynecologic surgery and to determine if such testing affects surgical outcomes.

Design: The American College of Surgeons National Surgical Quality Improvement Program (NSQIP) database was queried for ambulatory gynecologic surgeries between 2015 and 2018. Low-risk patients included in this study were defined as being American Society of Anesthesiologists class 1 or 2.

Absent Ureteral Efflux after Hysterectomy Leads to Diagnosis of Ureteral Atresia with Renal Atrophy.

Iatrogenic injury to the urinary system is a known complication of gynecologic surgery; therefore, intraoperative cystoscopy is frequently performed to assess for such injuries. However, if an abnormality is seen, the differential diagnosis extends beyond iatrogenic causes. A 42-year-old patient underwent a total abdominal hysterectomy and had absent efflux from the right ureteral orifice on cystoscopy.

Expression of KIR2DL3 and KIR2DS2 natural killer receptors in exercise.

The mechanisms of early activation of natural killers were studied in volunteers of different athletic qualification. The initial levels of expression of KIR2DL2, PRF1, and GZMB genes regulating the functions of natural killer differ in athletes and untrained subjects. Moderate exercise stimulates transcription activities of these genes.

Long-term exercises increase the concentration of HspBP1, a co-chaperone of 70-KDa heat shock protein.

Extracellular concentration of heat shock protein (Hsp) with a molecular weight of 70 kDa (Hsp70) rapidly increases in the serum in response to stress and returns to the basal level during recovery. Further regulation of its blood concentration is unclear. A possible regulator is HspBP1, a protein binding Hsp70.

Prediction of epitopes in closely related proteins using a new algorithm.

Latrophilin 1 (presynaptic receptor) binds α-latrotoxin from black widow spider venom and regulates neurotransmitter release from nerve endings. The study of the mechanism of action of this receptor is impeded by the existence of closely related latrophilins 2 and 3. A profile of differences detecting the most differing and identical sites in several proteins was developed in order to obtain highly specific antibodies for differentiation between isoforms of related proteins.

Assessment of biochemical characteristics of the saliva using Fourier transform mid-infrared spectroscopy.

We developed a method of rapid assay for biochemical properties of the saliva based on attenuated total reflection infrared spectroscopy. This method allows evaluating saliva composition within 5 min without sample preparation; 10 microl sample is enough for the analysis. The concentration of total protein, glucose, secretory immunoglobulin A, urea, amylase, cortisol, inorganic phosphate in the saliva can also be measured.

Effect of a six-hour marathon ultra-race on the levels of IL-6, LIF, and SCF.

The effects of 6-h marathon ultra-race (long aerobic work below the lactate threshold level) on the levels of IL-6, leukemia inhibiting factor (LIF), and stem cell growth factor (SCF) were studied. The athletes participating in the study had different endurance levels evaluated by the distance covered over 6 h. The level of IL-6 sharply increased after exercise.

Studying the copy number of ribosomal protein L7/L12.

Studying the copy number of ribosomal protein L7/L12 was performed using monoclonal antibody 3G9 to the linear epitope on the C-terminal domain of this protein from Escherichia coli. Immunohistochemical study showed that Agrobacterium tumefaciens ribosomes include 6 copies of protein L7/L12. Our results suggest that the copy number of this protein has an evolutionary role.