Transcriptome of D14 in vivo x in vitro bovine embryos: is there any difference?

It is well-established that in vitro culture affects quality, gene expression, and epigenetic processes in bovine embryos and that trophectoderm cells are the most susceptible to abnormalities. These changes have been reported as the main factors responsible for losses observed after transfer of in vitro-produced embryos. The present study aimed to investigate the effect of an in vitro system on bovine embryo transcriptional profiles on D14 of development.

DNA Methylation of the Insulin-Like Growth Factor 2-Imprinted Gene in Trophoblast Cells of Elongated Bovine Embryo: Effects of the Culture.

DNA methylation is an essential epigenetic mark for embryo development and can be susceptible to environment factors such as conditions. The aim of this study was to verify the effect of culture until Day (D) 14 of the development on the embryo size and DNA methylation pattern of the insulin-like growth factor ()-imprinted gene. To achieve this, we produced bovine embryos completely , completely , and until D7 and then up to D14.

Characterisation of the methylation pattern in the intragenic CpG island of the IGF2 gene in Bos taurus indicus cumulus cells during in vitro maturation.

Purpose: The aim of this study was to characterise the methylation pattern in a CpG island of the IGF2 gene in cumulus cells from 1-3 mm and  ≥ 8.0 mm follicles and to evaluate the effects of in vitro maturation on this pattern.

Methods: Genomic DNA was treatment with sodium bisulphite.

Post-hatching development of bovine embryos in vitro: the effects of tunnel preparation and gender.

The objective of this study was to compare morphological characteristics, kinetics of development, and gene expression of male and female IVP embryos that were cultured until day (D)15 (fertilization = D0), using either phosphate-buffered saline (PBS) or Milli-Q water (MQW) to dilute the agarose gel used for tunnel construction. On D11, embryos (n = 286) were placed in agarose gel tunnels diluted in PBS and MQW. Embryos were evaluated for morphology, and embryo size was recorded on D11, D12.