Publications by authors named "Grayson Gerlich"
Chlorothalonil (2,4,5,6-tetrachloroisophthalonitrile; TPN) is an environmentally persistent fungicide that sees heavy use in the USA and is highly toxic to aquatic species and birds, as well as a probable human carcinogen. The chlorothalonil dehalogenase from Pseudomonas sp. CTN-3 (Chd, UniProtKB C9EBR5) degrades TPN to its less toxic 4-OH-TPN analog making it an exciting candidate for the development of a bioremediation process for TPN; however, little is currently known about its catalytic mechanism.
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- The study examines how adding internal cavities to a protein affects its folding and stability, with a focus on the leucine-rich repeat protein pp32.
- Cavity creation in the N-terminal region caused significant deviations from normal unfolding behaviors, while alterations in the stable C-terminal half resulted in more synchronized unfolding.
- Surprisingly, increasing the size of a central cavity led to the emergence of a distinct partially unfolded state, highlighting that even small changes in cavity structure can significantly impact protein conformations.