One-year follow-up of kidney transplant recipients with increased expression of mRNA for granzyme B in urinary cells.

Background: Measurement of mRNA encoding cytotoxic proteins in urinary cells is recognized as a potential noninvasive means to diagnose acute rejection in kidney allograft recipients. We sought to evaluate kidney graft function after 1 year follow-up without therapeutic intervention among patients with increased urinary expression of mRNA for granzyme B, albeit with stable graft function at the time of measurements.

Patients And Methods: The 29 randomly selected patients were at a median of 39 months (range, 10-156) after transplantation with stable graft function over the previous 3 months.

Hyperacute rejection of living related kidney grafts caused by endothelial cell-specific antibodies: case reports.

We describe two cases of hyperacute humoral rejection of living related kidney grafts despite negative pretransplantation T- and B-lymphocyte flow cytometric crossmatches and blood group identity. Retrospectively, antiendothelial IgG antibodies were detected on a panel of umbilical cord cells in the first case, and IgM antibodies against donor endothelial precursor cells were detected using a new endothelial cell crossmatch kit in the second case. Standard crossmatch methods using donor lymphocytes failed to detect these pathogenic antibodies and did not predict the danger of hyperacute rejection.

The effects of occlusive techniques on the short-term prognosis after liver resections.

Background/aims: Anatomic liver resection can be performed without vascular occlusion, but controlling blood loss during liver parenchyma dissection by compression or clamping of vessels in the liver hilus is almost the rule. The aim of this study is to assess the negative consequences of different types of occlusion techniques used during liver parenchyma dissection.

Methodology: From 2001 to 2003, 43 anatomical liver resections were performed in patients with primary and metastatic tumors.

Hyperacute rejection of living related kidney graft caused by IgG endothelial specific antibodies with a negative monocyte cross-match.

We present a case report of a 30-year-old man, who hyperacutely rejected a blood group identical, HLA-haploidentical living related kidney graft in spite of the fact that he had never been immunized. Anti-endothelial IgG antibodies that did not react with monocytes were detected with flow cytometry, on a panel of human umbilical cord cells in his serum retrospectively. On the basis of this experience we put for consideration the possibility of regular examination of non-HLA antibodies in potential living graft recipients.

[Kidney transplantation from living donors].

From Jan. 1, 1994 till August 31, 1999 in the Transplantation Centre of the F. D.

[The reflex sympathetic dystrophy syndrome of the lower extremities in patients after kidney transplantation--another complication of cyclosporin A therapy?].

The authors describe a new complication observed in patients after transplantation of the kidneys, characterized by intensive periarticular pain of the joints of the lower extremities, mostly with a symmetrical affection of the heels and knees, associated with vasomotor changes in the affected area, X-ray evidence of patchy osteoporosis and an increased periarticular activity of radionuclide on bone scans. The symptoms develop shortly after transplantation and recede within several months. The clinical findings, X-ray and scintigraphic changes are typical for the syndrome of reflex sympathetic dystrophy.

Reflex sympathetic dystrophy of the lower limbs after kidney transplantation.

Reflex sympathetic dystrophy syndrome (RSDS) is a rarely described complication after different types of organ transplants. Three out of 147 kidney recipients treated at our center during the last 6 years developed severe bilateral symmetrical pain in the ankles and knees, with great difficulties in walking 2-3 months after kidney transplantation. Clinical examination revealed periarticular soft tissue swelling and vasomotor changes with no effusion.