Complete remission achieved in a case of both primary and recurrent adult acute myelogeneous leukemia by a novel nutritional therapy.

Objectives: The aim of this study was to determine the possible clinical benefit of molasses-based dietary compositions (designated as MSQ 13, MSQ 15, and MSQ 18) in a case of both primary and recurrent adult AML.

Design: The design was a single case study.

Settings/location: The setting was in the home.

[Are there sex differences in the prevalence of overweight and adiposity in elementary school children in Austria].

Based on the data of 203 male and 179 female schoolchildren from Eastern Austria (Burgenland), aged between 6 and 10 years, sex typical differences in body composition (absolute and relative body fat, lean body mass) and weight status were analyzed. Body composition analyses were carried out by means of BIA method, weight status was estimated using BMI percentiles (BMI > 90th percentile defined overweight, BMI > 97th percentile defined obesity). Statistically significant sex differences were found for all body composition parameters, girls exhibited a significantly higher amount of absolute and relative body fat, whereas their male counterparts exhibited a significantly higher amount of lean body mass.

The cancer stem cell: evidence for its origin as an injured autoreactive T cell.

This review explores similarities between lymphocytes and cancer cells, and proposes a new model for the genesis of human cancer. We suggest that the development of cancer requires infection(s) during which antigenic determinants from pathogens mimicking self-antigens are co-presented to the immune system, leading to breaking T cell tolerance. Some level of autoimmunity is normal and necessary for effective pathogen eradication.

Characterization of the DialGuard device for endotoxin removal in hemodialysis.

Bacterial pyrogens, capable of penetrating dialyzer membranes, are responsible for a systemic inflammatory reaction in hemodialysis patients. Dialyzer reuse, involving rinsing of the dialyzer with pyrogen-containing water, may exacerbate this situation. Studies of the mechanism of action of endotoxin suggest that it irreversibly damages the vascular endothelium.

Endotoxin removal from whole blood by a novel adsorption resin: efficiency and hemocompatibility.

The structural component of Gram- bacteria, endotoxin (ET), induces the release of endogenous mediators of sepsis. Attempts to remove these downstream molecules in vivo, have not improved survival. However, extracorporeal strategies such as continuous renal replacement therapy or therapeutic plasmapheresis have shown benefit.

A new method for removing endotoxin from plasma using hemocompatible affinity chromatography technology, applicable for extracorporeal treatment of septic patients.

The pathogenesis of sepsis begins with the proliferation of micro-organisms at a site of infection, followed by invasion of the bloodstream and other organs. Gram-negative bacteria account for a large part of sepsis cases. The structural component of Gram-negative bacteria, endotoxin or lipopolysaccharide (LPS), induces the synthesis and release of endogenous mediators of sepsis.

Cancer: a single disease with a multitude of manifestions?

The relationships of critical nutrients such as plant phenolics, vitamins, minerals and lipids are considered with respect to the incidence of a variety of cancers, and analyzed in terms of how these nutrient deficiencies alter immune function, DNA integrity and cell proliferation. With a significant correlation found between cancer and these nutrient deficiencies, the hypothesis is presented here that nutrition could provide a unifying perception of cancer and recast it as a single disease. This further suggests that a coordinated administration of specific, critical nutrients to cancer patients could lead to the reversal of the disease.

Integration of cell culture with continuous, on-line sterile downstream processing.

The development of an integrated system for the continuous, automated production of pure cell culture derived proteins is discussed. The system comprises a cell culture subunit for the continuous culture of mammalian cells and a purification subunit linked on-line to the cell culture subunit. The cells are compartmentalized and continuously perfused with culture medium.

Phosphorylation in vivo of rat hepatic glucocorticoid receptor.

Rat liver glucocorticoid receptors were labeled in vivo with [32P]orthophosphate. In the last two fractionation procedures leading to purified, molybdate-stabilized, unactivated receptor complex, bound [32P] coeluted with peaks of bound [3H]triamcinolone acetonide. SDS-gel electrophoresis revealed [32P] labeled 90K and 24K bands.

Purification of the unactivated glucocorticoid receptor and its subsequent in vitro activation.

The unactivated, molybdate-stabilized rat hepatic glucocorticoid receptor has been purified approximately 4000-fold, as calculated by specific radioactivity, by affinity chromatography using a deoxycorticosterone-derivatized agarose, gel filtration on Bio-Gel A-1.5m agarose, and DEAE-cellulose chromatography. The final receptor sediments at 9-10 S in low salt (40 mM KCl) glycerol gradients containing molybdate.

Monoclonal antibodies to the glucocorticoid receptor.

One thousand-fold purified unactivated rat liver glucocorticoid receptor was obtained by affinity chromatography followed by gel filtration. Splenic lymphocytes of immunized mice were fused with a nonproducer SP2 myeloma cell line to obtain hybridomas secreting antibodies against receptor. Hybridoma lines, injected into mice, developed ascites tumors which provide substantial amounts of monoclonal antibodies.

Purification of "nontransformed" avian progesterone receptor and preliminary characterization.

The nontransformed (molybdate-stabilized) avian oviduct progesterone receptor has been purified to near homogeneity by a simple four-step procedure: ammonium sulfate fractionation, affinity chromatography, gel filtration, and DEAE-Sephadex A-25 chromatography. The affinity resin (deoxycorticosterone-agarose) is very resistant to chemical breakdown and enzymatic destruction and can be used repeatedly. The material obtained after gel filtration was separated into two receptor components by DEAE-chromatography.

A new affinity resin for purification of non-transformed avian progesterone receptor.

A new steroid affinity resin has been developed which can be successfully used to partially purify the non-transformed species of the progesterone receptor from chick oviduct. Deoxycorticosterone has been modified through its 21-carbon and linked to a stable spacer arm which is attached to Sepharose 2B by an epoxide technique. The affinity resin is very resistant to chemical breakdown or to enzymatic destruction in crude tissue extracts.

Preparation of polysaccharide derivatives containing amino groups for application in affinity chromatography.

We describe here simple methods to introduce spacer arms with amino functional group into polysaccharides. (Formula: see text) The technique includes the following steps: Compounds comprising epoxide group(s) are introduced into polymer supports to form the spacers. Then, reactive groups on the other side of the introduced spacers are reacted with ammonia.