Neoadjuvant radiotherapy followed by hysterectomy in locally advanced endometrial cancer: Outcomes from a tertiary government hospital in the Philippines.

Objective: Managing endometrial cancer with suspected or gross cervical involvement lacks a standard approach. This study evaluated outcomes in patients with cervical and/or parametrial involvement treated with neoadjuvant radiation followed by hysterectomy.

Methods: Fourteen patients from 2007 to 2022 with locally advanced endometrial cancer and cervical and/or parametrial involvement were retrospectively analyzed.

COVID-19 in multiple sclerosis and neuromyelitis optica spectrum disorder patients in Latin America: COVID-19 in MS and NMOSD patients in LATAM.

Background: There is no data regarding COVID-19 in Multiple Sclerosis (MS) and neuromyelitis optica spectrum disorder (NMOSD) patients in Latin America.

Objective: The objective of this study was to describe the clinical characteristics and outcomes of patients included in RELACOEM, a LATAM registry of MS and NMOSD patients infected with COVID-19.

Methods: RELACOEM is a longitudinal, strictly observational registry of MS and NMOSD patients who suffer COVID-19 and Dengue in LATAM.

Dissemination of a multidrug resistant CTX-M-65 producer Salmonella enterica serovar Infantis clone between marketed chicken meat and children.

The objective of the present study was to characterize Salmonella enterica serovar Infantis isolated from chicken meat determining their clonal relationships with S. Infantis isolated from children with diarrhea. Fifteen meat-recovered S.

New combinations and synonyms in discoid caespitose Andean (Senecioneae, Compositae).

The names and are transferred to the genus . They belong to the group of the discoid caespitose Andean , specifically to the subgroup with blackish anthers and style branches and whitish corollas. The recognition of as a distinct species is also discussed.

Rad7 E3 Ubiquitin Ligase Attenuates Polyubiquitylation of Rpn10 and Dsk2 Following DNA Damage in .

During Nucleotide Excision Repair (NER) in the yeast , ubiquitylation of Rad4 is carried out by the E3 ubiquitin ligase that includes Rad7-Elc1-Cul3 and is critical to optimal NER. Rad7 E3 activity targets Rad4 for degradation by the proteaseome but, in principle, could also trigger other DNA damage responses. We observed increased nuclear ubiquitin foci (Ub-RFP) formation in containing a Rad7 E3 ligase mutant () in response to DNA damage by benzo[a]pyrenediolepoxide (BPDE) in dividing cells.

Identification of genes involved in imatinib resistance in CML: a gene-expression profiling approach.

The use of the tyrosine kinase inhibitor imatinib, which blocks the enzymatic action of the BCR-ABL fusion protein, has represented a critical advance in chronic myeloid leukemia (CML) treatment. However, a subset of patients initially fails to respond to this treatment. Use of complementary DNA (cDNA) microarray expression profiling allows the identification of genes whose expression is associated with imatinib resistance.

An improved description of Strombidium sulcatum Claparède & Lachmann, 1859 (Ciliophora) from slides of Fauré-Fremiet, and a designation of type material.

Strombidium sulcatum, the type species of the genus, is examined using silver-stained material made by Fauré-Fremiet in the 1950s and 1960s. We found that the stained specimens possessed a prominent protuberance, as previously described; the number of anterior polykinetids (APk) differed between the accounts (15 this study, 12 previous reports); although previously illustrated, no contractile vacuole was observed; and the macronuclear shape, position, and size differed from those previously reported. Our work provides the first detailed re-analysis of the silver-stained material of S.

Chronic myeloid leukemia patients resistant to or intolerant of interferon alpha and subsequently treated with imatinib show reduced immunoglobulin levels and hypogammaglobulinemia.

Background And Objectives: Imatinib mesylate inhibits ABL tyrosine kinase. This protein serves a complex role in cell cycling and is important in lymphopoiesis. We describe the immunologic findings in patients with chronic myeloid leukemia resistant to or intolerant of interferon (IFN) a who were treated with imatinib.

Multiplied functions unify shapes of ganglion-cell receptive fields in retina of turtle.

Retinal ganglion cells in the turtle were extracellularly recorded to define the shapes of their receptive fields by small moving light spots. To better define the geometries, spectral-light adaptations and vitreal injections of 2-amino-4-phosphonobutyric acid (APB) were used to disrupt balances in field organization along dimensions of wavelength, ON and OFF responses, and center/surround areas. Three-dimensional data plots were fit by Gaussian, Gabor, and cardioid functions to show that the shapes of receptive fields are predicted by combinations of these multiplied functions.

Different patterns of relapse after autologous peripheral blood stem cell transplantation in multiple myeloma: clinical results of 280 cases from the Spanish Registry.

Background And Objectives: Autologous peripheral blood stem cell (PBSC) transplantation is widely used to treat patients with multiple myeloma (MM). However, only a small fraction of patients remain free of disease in the long-term and most patients will finally relapse. The clinical presentation of relapse after transplantation is very heterogeneous and few reports have analyzed this situation.

[Treatment of leukemia relapsed after allogenic bone marrow transplantation with donor lymphocyte infusion: report of 11 cases].

Purpose: Donor leukocyte infusions (DLI) are useful for treating leukaemic relapse after allogeneic bone marrow transplantation (BMT). We reviewed our experience with eleven patients who received DLI between 1995 and 1997.

Patients And Methods: The diagnoses prior to DLI were: chronic myeloid leukaemia (CML) in chronic phase (CP) (two patients) or accelerated phase (two patients), acute myeloid leukaemia (AML) (two patients), acute lymphoid leukaemia (ALL) (two patients), and refractory anaemia with excess blasts under transformation (tRAEB) (three patients).

Balanced interactions in ganglion-cell receptive fields.

Receptive fields of retinal ganglion cells in turtle have excitatory and inhibitory components that are balanced along the dimensions of wavelength, functional ON and OFF responses, and spatial assignments of center and surround. These components were analyzed by spectral light adaptations and by the glutamate agonist, 2-amino-4-phosphonobutyric acid (APB). Extracellular recordings to stationary and moving spots of light were used to map changes in receptive fields.

Comparison of peripheral blood progenitor cell mobilization in patients with multiple myeloma: high-dose cyclophosphamide plus GM-CSF vs G-CSF alone.

The best method for peripheral blood progenitor cell (PBPC) mobilization in patients with multiple myeloma (MM) remains controversial. We report the results of two different methods of PBPC collection for autologous transplantation in 40 patients with stage II or III MM. In group I (n = 18), HD-CY, 4 g/m2 i.

Quinton-Mahurkar catheter as short-term central venous access for PBSC collection: single-center experience of 370 aphereses in 110 patients.

We retrospectively analyzed our experience with the Quinton-Mahurkar dual-lumen hemodialysis catheter as short-term central venous access for harvesting peripheral blood stem cells (PBSC) for autologous transplantation. For intensification therapy for various malignancies 370 leukaphereses were performed in 110 candidates. The catheter was placed percutaneously under local anesthesia only for the time of blood collection and in no case was it used for the PBSC transplant.

Pediatric experience with autologous peripheral blood progenitor cell transplantation: influence of CD34+ cell dose in engraftment kinetics.

The aim of this study was to analyze factors affecting mobilization and engraftment in 40 children undergoing autologous peripheral blood progenitor cell transplantation for different malignancies: 19 patients with haematological malignancies and 21 patients with solid tumors. Patients received 4-5 days of rhG-CSF (12 micrograms/kg/day) subcutaneously. Apheresis was performed by continuous flow blood cell separation beginning on the fifth day of rhG-CSF.

Allogeneic peripheral blood progenitor cell (PBPC) transplantation in children.

The use of peripheral blood as a source of hematopoietic precursors is an alternative to the bone marrow in allogeneic transplantation. Although pediatric allogeneic PBPC experience is limited, there is no reason to believe that the outcome and benefit with PBPC should be different than adults. We describe our initial experience in two children who received PBPC allogeneic transplantation in whom the donors were mobilized with filgrastim.

Collection and transplantation of peripheral blood progenitor cells mobilized by G-CSF alone in children with malignancies.

Results of collection and transplantation of peripheral blood progenitor cells (PBPC) mobilized by G-CSF in 31 children with different malignancies were analysed. A total of 43 aphereses were performed, following administration of granulocyte colony-stimulating factor (G-CSF), using a continuous flow blood cell separator (Cobe Spectra) through a central venous catheter. For patients weighing View Article and Find Full Text PDF

Large-volume leukapheresis for peripheral blood stem cell collection in children: a simplified single-apheresis approach.

Large volume leukapheresis (LVL) defined as the processing of greater than three volumes of blood in a single session for peripheral blood stem cell (PBSC) collection was performed in 27 children, aged from 1 to 15 years, with various malignancies. Harvesting of PBSC was started after 4 days of cytokine (G-CSF 12 micrograms/kg s.c.

The pupillary response to light in the turtle.

When intense adapting lights are turned off, the pupil of the turtle, Pseudemys scripta elegans, enlarges. The recovery functions for pupillary dilation have different time constants that are defined by red- and green-sensitive cones and rods as they are affected by prior light adaptation and time in the dark. Pupillary area related to dilation responds over at least a three- to four-fold range.

Functional morphologies of retinal ganglion cells in the turtle.

Retinal ganglion cells in the turtle, Pseudemys scripta elegans, were examined by intracellular recording with a protocol of stationary and moving lights. Responses were apportioned among OFF, ON, and ON-OFF categories, and directional selectivity. Cells were injected with Neurobiotin, then later conjugated with avidin-horseradish peroxidase in standard procedure.

Ocular dimensions and schematic eyes of freshwater and sea turtles.

Measurements were made of the ocular dimensions from living and frozen eyes of one species of freshwater turtle, Pseudemys scripta elegans, and of three species of marine turtles, Chelonia mydas, Dermochelys cariacea, and Eretmochelys imbricata. Estimates of refractive error by retinoscopy were also obtained with eyes in air and under water. The results suggest that unaccommodated eyes of all four species are approximately emmetropic in air but strongly hyperopic in water.

Refractive state, contrast sensitivity, and resolution in the freshwater turtle, Pseudemys scripta elegans, determined by tectal visual-evoked potentials.

Visual-evoked potentials (VEPs) were recorded from the surface of the optic tectum of the freshwater turtle, Pseudemys scripta elegans, in response to phase reversal of square-wave gratings of different spatial frequency and contrast. The refractive state of a group of 12 turtles in air was assessed from VEPs by placing trial lenses in front of the eye. The group mean refraction did not differ significantly from emmetropia, as compared to 4.

Wavelength-dependent temporal properties of retinal horizontal cells in turtles.

Electrical responses of luminosity horizontal cells (L cells) to monochromatic stimuli were analyzed by intracellular recordings in the retinas of the freshwater turtle (Pseudemys scripta elegans) and of the sea turtle (Chelonia mydas mydas). Light intensity, duration, and wavelength were varied to assess temporal effects. For a given intensity of monochromatic light, response amplitude increased with stimulus duration until maximum amplitude occurred at a specific duration.

Classification of turtle retinal ganglion cells.

1. Receptive fields of 78 retinal ganglion cells were analyzed for their responses to moving and stationary lights that were presented under a variety of stimulus conditions. All cells were sensitive to moving stimuli, and their receptive fields often comprised excitatory and inhibitory sub-regions.