Publications by authors named "Graeme Summers"
Article Synopsis
- Norovirus is the main cause of viral gastroenteritis worldwide, mainly spreading through person-to-person contact and contaminated food, especially shellfish.
- This study aims to assess norovirus infectivity in shellfish using reverse transcription quantitative PCR (RT-qPCR) and a specific dye (PMAxx-RT-qPCR) to analyze viral integrity after high-pressure processing (HPP).
- Results showed increasing non-infectious virus ratios with higher pressure and temperature conditions, indicating that high-pressure treatments can reduce the integrity and infectivity of norovirus in shellfish.
A laboratory-based study testing 9 strains independently and a cocktail of 11 strains was carried out. The aim was to identify suitable strain(s) to model in inactivation experiments. Three separate inactivation procedures and a hurdle combination of the three were employed: thermal inactivation (55°C), UV-C irradiation (245 nm), and chemical sanitizer (Tsunami 100, a mixture of acetic acid, peroxyacetic acid, and hydrogen peroxide).
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