Far-Field Super-Resolution Vibrational Spectroscopy.

Potential label-free alternatives to super-resolution fluorescence techniques have been the focus of considerable research due to the challenges intrinsic in the reliance on fluorescent tags. In this Feature, we discuss efforts to develop super-resolution techniques based on vibrational spectroscopies and address possible sample applications as well as future potential resolution enhancements.

Optimized Ki-67 staining in murine cells: a tool to determine cell proliferation.

The reliable analysis of the cell cycle status has become increasingly relevant for scientific and clinical work, especially for the determination of tumor cell growth. One established method to characterize the proliferation activity of cells is the analysis of the Ki-67 protein. Ki-67 is expressed in the nucleus during the whole cell cycle except for the G0 phase.

Evaluating gradient artifact correction of EEG data acquired simultaneously with fMRI.

Simultaneous electroencephalography (EEG) and functional magnetic resonance imaging (fMRI) has become a widely used application in spite of EEG perturbations due to electromagnetic interference in the MR environment. The most prominent and disturbing artifacts in the EEG are caused by the alternating magnetic fields (gradients) of the MR scanner. Different methods for gradient artifact correction have been developed.

Arteriovenous fistula presenting as a tumor of the upper eyelid.

We report, to our knowledge for the first time, a case of arteriovenous fistula presenting as a tumor of the upper eyelid in an 80-year-old patient. Surgical excision through an anterior approach brought excellent cosmetic and functional results.

Beta 2-adrenergic receptors on peripheral blood mononuclear cells in patients with rheumatic diseases.

In order to investigate the influence of the autonomic nervous system on the immune response in rheumatic diseases such as rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE), we determined the number and the dissociation constants of beta 2-adrenergic receptors (beta 2R) on peripheral blood mononuclear cells (PBMC) in patients with RA characterized by either low or high disease activity, patients with SLE, and healthy age-matched controls by means of a radioligand binding assay with [125I]iodocyanopindolol. The number of beta 2R was significantly decreased in all three patient groups as compared with the healthy controls. In SLE patients, a significant negative correlation between beta 2R and the anti-ds-DNA antibody titre was observed (r = -0.

Decreased density of beta-adrenergic receptors on peripheral blood mononuclear cells in patients with rheumatoid arthritis.

There is growing evidence that the autonomic nervous system influences the immune response by activation and modulation of beta 2-adrenergic receptors (beta 2R) on immunocompetent cells. However, little is known about its pathogenetic role in autoimmune disease. Therefore, the number and the dissociation constants (beta 2R-KKD) of beta 2R on peripheral blood mononuclear cells (PBMNC) were determined in 21 patients with rheumatoid arthritis (RA) and 9 healthy age and sex matched controls [healthy donors (HD)] by a radioligand binding assay with [125I]iodocyanopindolol.