Solid-phase spectrofluorimetric determination of acetylsalicylic acid and caffeine in pharmaceutical preparations using partial least-squares multivariate calibration.

PLS-1, a variant of the partial least-squares algorithm was used for the solid-phase spectrofluorimetric determination of acetylsalicylic acid (ASA) and caffeine (CF) in pharmaceutical formulations. The method allows the simultaneous quantification of the analytes, as the closely overlapping spectral bands are efficiently solved. Sample preparation prior to analysis is not required.