Publications by authors named "Grace Krohn"
Article Synopsis
- Microphysiological and organ-on-chip platforms aim to improve human disease models and drug development due to low success rates in clinical interventions.
- Current platforms often depend on animal or synthetic materials that fail to accurately replicate human physiology, especially in diseases influenced by the extracellular matrix.
- The study introduces human cell-derived matrix (hCDM) hydrogels, which enhance the modeling of blood vessels by using components from human fibroblasts, promoting better vascularization and more effective disease modeling.
Vascular Ehlers-Danlos Syndrome (vEDS) is a rare autosomal dominant disease caused by mutations in the COL3A1 gene, which renders patients susceptible to aneurysm and arterial dissection and rupture. To determine the role of COL3A1 variants in the biochemical and biophysical properties of human arterial ECM, we developed a method for synthesizing ECM directly from vEDS donor fibroblasts. We found that the protein content of the ECM generated from vEDS donor fibroblasts differed significantly from ECM from healthy donors, including upregulation of collagen subtypes and other proteins related to ECM structural integrity.
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