Biomarkers.

Background: Apolipoprotein ε4 allele (APOE4) is the strongest genetic risk factor for late-onset Alzheimer's disease (AD). Compared with non-carriers, cognitively normal APOE4 individuals have shown brain atrophy and lower cerebral blood flow (CBF) decades before AD pathological and clinical symptoms appear. The goal of the study is to determine if using Sirolimus, an FDA-approved mTOR inhibitor, could restore the brain volumes in structures related to cognitive functions and global CBF (gCBF) for asymptomatic APOE4 carriers compared with non-carriers.

Alzheimer's Imaging Consortium.

Background: Apolipoprotein e4 allele (APOE4) is the strongest genetic risk factor for late-onset Alzheimer's disease (AD). Compared with non-carriers, cognitively normal APOE4 individuals have shown brain atrophy and lower cerebral blood flow (CBF) decades before AD pathological and clinical symptoms appear. The goal of the study is to determine if using Sirolimus, an FDA-approved mTOR inhibitor, could restore the brain volumes in structures related to cognitive functions and global CBF (gCBF) for asymptomatic APOE4 carriers compared with non-carriers.

The rare cases of pneumatic colorectal perforation: A cautionary tale of compressed air misuse.

Barotrauma is a medical condition caused by sudden pressure changes in the body causing damage to multiple parts of the body. However, it is an infrequent occurrence when it comes to Colo-rectal perforation, wherein the trauma occurs due to the insufflation of compressed air through the anus. Several factors influence the outcome of a patient with colonic perforation due to barotrauma such as the severity of the injury, hemodynamic status of the patient, the patient's general health and well-being, the time taken for active medical/ surgical intervention since the injury, aggressive intravenous antibiotics administration to prevent sepsis, post-operative complications like surgical site infection, post-op ileus, anastomotic leak, etc.

Beyond the virus: ecotoxicological and reproductive impacts of SARS-CoV-2 lysate protein in C57Bl/6j female mice.

Since the establishment of the COVID-19 pandemic, a range of studies have been developed to understand the pathogenesis of SARS-CoV-2 infection, vaccine development, and therapeutic testing. However, the possible impacts that these viruses can have on non-target organisms have been explored little, and our knowledge of the consequences of the COVID-19 pandemic for biota is still very limited. Thus, the current study aimed to address this knowledge gap by evaluating the possible impacts of oral exposure of C57Bl/6 J female mice to SARS-CoV-2 lysate protein (at 20 µg/L) for 30 days, using multiple methods, including behavioral assessments, biochemical analyses, and histopathological examinations.

Molecular correlates for HPV-negative head and neck cancer engraftment prognosticate patient outcomes.

There is a pressing need to improve risk stratification and treatment selection for HPV-negative head and neck squamous cell carcinoma (HNSCC) due to the adverse side effects of treatment. One of the most important prognostic features is lymph nodes involvement. Previously, we demonstrated that tumor formation in patient-derived xenografts (i.

Toxicity assessment of Oreochromis mossambicus exposed to carbamazepine and selenium: Physiological and genotoxic approach.

Although the toxicity of selenium (Se) and carbamazepine (CBZ) has already been demonstrated, the possible effects of freshwater fish co-exposure to these pollutants have not been explored. Thus, we aimed to evaluate the potential impact of Se and CBZ (alone and combined) exposure (both 5 µg/L) in Oreochromis mossambicus after 28 days. Exposure to CBZ, alone or combined with Se, significantly increases the "red blood cells" and "mean corpuscular volume.

Protocol for generating high-fidelity proteomic profiles using DROPPS.

Deep mass spectrometry-based proteomic profiling of rare cell populations has been constrained by sample input requirements. Here, we present a protocol for droplet-based one-pot preparation for proteomic samples (DROPPS), an accessible low-input platform that generates high-fidelity proteomic profiles of 100-2,500 cells. We describe steps for depositing cellular material, cell lysis, and digesting proteins in the same microliter-droplet well.

Marine macroalgae Chaetomorpha aerea as a dietary supplement: Optimizing immunity and resistance to Edwardsiella tarda in tilapia (Oreochromis mossambicus).

The intensification of aquaculture has led to a rise in fish infections, necessitating the search for alternative antibiotics. In this context, our study investigated the effects of dietary supplementation with Chaetomorpha aerea, a filamentous green algae, on the immune health and resistance to infections in tilapia (Oreochromis mossambicus). Diets containing varying concentrations of C.

A convenient strategy for mitigating microplastics in wastewater treatment using natural light and ZnO nanoparticles as photocatalysts: A mechanistic study.

Polypropylene microplastics (PPMPs) are one of the major emerging contaminants in the ecosystem due to their frequent usage and improper disposal practices. These PPMPs enter ecosystems via wastewater effluent plants and cause severe environmental health issues. In addition, quantifying PPMPs smaller than 50 μm in wastewater plant extraction is very difficult.

Impact of Chaetomorpha aerea-enriched diet on growth, feed utilization, and haemato-immunological responses in Clarias batrachus challenged with Aeromonas hydrophila.

The bacteria Aeromonas hydrophila, which causes motile Aeromonas septicemia (MAS), is dangerous to aquaculture because it affects the fish's well-being and production. As the aquaculture industry seeks sustainable and effective methods to enhance fish immunity and growth, natural supplements such as marine algae have gained attention. This study explored the potential benefits of incorporating the green marine algae Chaetomorpha aerea into the fish diet, focusing on disease resistance, growth, feed utilization, and hematological and immunological responses.

Eco-toxicity assessment of polypropylene microplastics in juvenile zebrafish (Danio rerio).

In recent years, everyone has recognized microplastics as an emerging contaminant in aquatic ecosystems. Polypropylene is one of the dominant pollutants. The purpose of this study was to examine the effects of exposing zebrafish (Danio rerio) to water with various concentrations of polypropylene microplastics (11.

Development of Biodegradable Bioplastics with Sericin and Gelatin from Silk Cocoons and Fish Waste.

The bioplastics sector promotes environmentally friendly means of cutting down on the usage of fossil fuels, plastic waste, and environmental pollution. Plastic contamination has detrimental effects on both ecological systems and the global food supply. The approach we present here to resolve this issue involves the integration of sericin and gelatin, obtained from cocoon and fish waste, respectively, with nano-reinforced cellulose crystals, to develop a biodegradable and compostable plastic material.

Corrigendum to Spectroscopic properties (FT-IR, NMR and UV) and DFT studies of amodiaquine [Heliyon Volume , Issue 12, December 2023, e22187].

[This corrects the article DOI: 10.1016/j.heliyon.

Droplet-based proteomics reveals CD36 as a marker for progenitors in mammary basal epithelium.

Deep proteomic profiling of rare cell populations has been constrained by sample input requirements. Here, we present DROPPS (droplet-based one-pot preparation for proteomic samples), an accessible low-input platform that generates high-fidelity proteomic profiles of 100-2,500 cells. By applying DROPPS within the mammary epithelium, we elucidated the connection between mitochondrial activity and clonogenicity, identifying CD36 as a marker of progenitor capacity in the basal cell compartment.

Organelle resolved proteomics uncovers PLA2R1 as a novel cell surface marker required for chordoma growth.

Chordomas are clinically aggressive tumors with a high rate of disease progression despite maximal therapy. Given the limited therapeutic options available, there remains an urgent need for the development of novel therapies to improve clinical outcomes. Cell surface proteins are attractive therapeutic targets yet are challenging to profile with common methods.

Therapeutic mitigation of measles-like immune amnesia and exacerbated disease after prior respiratory virus infections in ferrets.

Measles cases have surged pre-COVID-19 and the pandemic has aggravated the problem. Most measles-associated morbidity and mortality arises from destruction of pre-existing immune memory by measles virus (MeV), a paramyxovirus of the morbillivirus genus. Therapeutic measles vaccination lacks efficacy, but little is known about preserving immune memory through antivirals and the effect of respiratory disease history on measles severity.

Bacterial screening in Indian coastal regions for efficient polypropylene microplastics biodegradation.

Polypropylene based medical devices significantly increased production and usage in COVID-19 pandemic states, and this material is very resilient in the environment. Thus, more than ever, rapid action is needed to reduce this pollution. This study focuses on the degradation of polypropylene microplastics (PP MPs) by unique marine bacterial strains obtained from the Thoundi (Bacillus tropicus, Bacillus cereus, Stenotrophomonas acidaminiphila, and Brucella pseudintermedia) and Rameshwaram coasts (Bacillus cereus).

Ni-Fe bimetallic catalysts with high dispersion supported by SBA-15 evaluated for the selective oxidation of benzyl alcohol to benzaldehyde.

A wetness impregnation method was used to impregnate the substrate with a substantial quantity of oleic acid together with a metal precursor, leading to significantly dispersed Ni-Fe bimetallic catalysts based on mesoporous SBA-15. Using a wide variety of characterization methods, such as XRD, BET, and TEM Analysis, the physiochemical properties of the catalyst were determined. The addition of the metal does not have any effect on the structural characteristics of the SBA-15 catalyst, as validated by transmission electron microscopy (TEM), which shows that the prepared SBA-15 supported catalyst has a hexagonal mesoporous structure.

Spectroscopic properties (FT-IR, NMR and UV) and DFT studies of amodiaquine.

Amodiaquine (AQ) was synthesized by a condensation reaction and characterized by experimental FT-IR, H and C nuclear magnetic resonance (NMR) and UV spectroscopies. In the present work, Density Functional Theory (DFT) calculations. The structural and spectroscopic (FT-IR, H and C NMR and UV) data of amodiaquine molecule in ground state have been investigated by using Density Functional Theory (DFT).