Effects of an organosilicon compound on the tubular apparatus of rat kidney. A histological and enzyme histochemical report.

Male adult Wistar rats were treated for 8 weeks with an organosilicone [2,2-dimethyl-4-(chloromethyl)-1,3-dioxa-2-silacyclopentane] employing two different dosages (25 and 50 mg/kg body weight i.p. daily) and the changes in the tubular apparatus of the kidney were investigated employing histological and enzyme-histochemical methods.

Biochemical and histochemical studies on the pituitary-testicular axis in obese (C57Bl/6J-ob/ob) mice.

Male C57Bl/6J-ob/ob mice (4 months old) and their homozygous lean controls were compared with respect to pituitary LH secretion and functional parameters of purified Leydig cells in vitro. Compared with controls, obese mice showed reductions in the following parameters: Plasma testosterone levels (reduced by 57%), hCG-stimulated testosterone formation in vitro (by 31%), conversion of progesterone to androgens by Leydig cells (by 39%), and GnRH-stimulated LH secretion (by 26%). Lipid accumulation and a 37% decrease in naphthylesterase activity in the Leydig cells as well as hyperplasia of pituitary gonadotrophs were observed histochemically in obese mice.

Anti-thyroid activity of purified thymus gland extract in male Wistar rats.

The effect of a purified bovine thymus gland extract (Dr. Kurt Mulli, GmbH, Hamburg, West Germany) was studied in 12-week old male Wistar rats on the following: thyroid weights and morphology, T3-T4 serum levels, thyroid lactic dehydrogenase, ATP-ase, acid phosphatase, and non-specific esterase activities. Thymus extract was administered intramuscularly daily for 21 days at doses of 0.

Enzyme histochemical studies on the pathological changes in human Sertoli cells.

Two forms of human Sertoli cell disorders were characterized enzyme histochemically from the testicular biopsy material of infertile and subfertile patients. Sertoli cell asthenia: a slight injury of the Sertoli cell with exfoliation of individual germ cells; marked by the rarefaction of reaction zones of thiamine pyrophosphatase (TPPase) and a decrease in lactate dehydrogenase (LDH). Sertoli cell insufficiency: severe Sertoli cell damage with the formation of a "puff" and a heavy exfoliation of germ cells (dislocation of Sertoli cell nucleus and cytoplasm along with the related germ cells into the lumen of seminiferous tubule); marked by a heterogeneous activity pattern of TPPase, the disappearance of LDH, maintenance of a slightly weakened activity of alkaline phosphatase, and an increase of acid phosphatase.

[Seasonal changes in the adenosine triphosphatase activity in the germinal cysts of the testis of the common frog Rana temporaria. An enzyme histochemical study].

Calcium activated ATP-ase was studied enzymohistochemically in the testis of the common frog Rana temporaria during an annual reproduction cycle. The enzyme activity was localized in the follicle cells of the cyst wall, in the mature Sertoli cells, in germ cells and in the lamina propria of the seminiferous tubules. The maximum activity was recorded during the hibernation period, the cyst formation and the late maturation phase.

Enzyme histochemical, biochemical and metabolic effects of Solcoseryl in connection with its radioprotective capability.

The lethal irradiation with gamma-rays (LD 100/30) of adult mice at ambient temperature of 32.5 degrees C heavily alterates the pattern of LDH in liver. In close correspondence with the radioprotective effect of Solcoseryl on the survival rate of irradiated mice -- which amounts to 100% as compared with 40% survival of solely heat adapted animals -- Solcoseryl normalizes the LDH distribution both histochemically and biochemically.

The action of Solcoseryl on two glycolytic enzymes of several organs of the irradiated mouse.

The blood extract Solcoseryl (SO), when administered daily in i.p. injection to mice adapted to ambient temperature of 32.

[Comparative histological and histochemical studies on the "spleen-ovary" in the rat (Lipschütz) following administration of FSH and spleen extract].

On the basis of the "Lipschuetz-ovary" model the rat ovary is cystically altered. -- In such an ovary the effect of a spleen extract (Solcosplen) and FSH (Anternon) were compared with histological and histochemical methods. -- With bothe the substances could be observed a diminution of the cystic degeneration.

[Enzyme-histochemical behaviour of the tanycyte ependyma in the III. ventricle of the rat with thiamin deficiency (author's transl)].

The influence of experimental thiamine deficiency in rats on enzyme-histochemical parameters was carried out in the diencephalon. The degree of thiamine deficiency was measured by the activity coefficient of transketolase in the red blood cells. The alpha-ETK of thiamine deficient animals was 1,45 +/- 0,36, of equal-fed animals 1,04 +/- 0.

[The reactive behaviour of the cell nucleus after a pretreatment with a bromide solution and the application of the calcium-cobalt-method for the ATPase evidence (author's transl)].

A selective staining of the nucleus of both the cells in an islet (of Langerhans) and the acinar cells could be observed after treatment with bromide-water in an ATPase-medium (calcium-cobalt-method) on a cryostatsection of rat pancreas. Furthermore the behaviour of the other metal ion forming a complex has been tested in place of cobalt salt. As conditioned for the staining reaction the hydrolysis of nucleic acid with oxidation of its base by bromide-water and also the complex formation of Co++ with compounds similar to alloxan, resulted from oxidation were discussed.

[Histochemical investigations in the experimental unilateral cryptorchism of the dog after HCG-treatment (author's transl)].

The behavior of various enzymes in the orthotopic and dystopic dog testis was examined under the conditions of experimental unilateral cryptorchism after HCG-treatment over a period of 3 months. The following findings could be obtained: 1. Under the HCG-application the orthotopic impuberal testis there was a change of behavior of the enzyme in the tubuli and Leydig-cells in comparison with test animals.