[Current problems of microbial safety of the interior environment of orbital stations after extended period of operation].

The authors give considerations to one of the core hygienic problems arising in the process of long-term operation of orbital stations, i.e. ensuring microbial health of the milieu interior.

[Electroencephalographic analysis of the central action of halonal].

The experiments on rabbits revealed the depressant effect of anticonvulsant drug halonal on spontaneous bioelectrical activity and electroexcitability of the motor area of the cerebral cortex, caudate nucleus, intralaminar nuclei of the thalamus and midbrain reticular formation. The drug exerts no effect on the amygdala and hippocamp. The mechanism of the anticonvulsant effect of halonal involves inhibition of the caudo-thalamo-cortical synchronizing system that presumably disorders the occurrence of the hypersynchronous charge in the cortical neurons and prevents the development of a convulsive fit.

[Characteristics of the functional antigens of Neisseria meningitidis group B].

The work deals with the characterization of the functional antigens of N. meningitidis, group B. Analysis of the total extract of N.

[Use of a new adhesive laminate in the production of contact lenses].

In the production of contact lenses for vision correction, the economic effect is expected as a consequence of replacing a foreign-made strip material by domestic strip laminate. The effect will continuously increase as the production grows up.

[Surface antigen structure of Neisseria meningitidis. I. The isolation of a serotypic antigenic complex of N. meningitidis strain B16B6 by direct detergent treatment and its immunochemical characteristics].

The method for obtaining a serotyping antigenic complex from N. meningitidis B16B6 by their direct treatment with the mixture of detergents (0.5% sodium desoxycholate and 0,5% cholic acid in the proportion 1 : 1) in 0.

[Effect of barbiturates on the respiratory activity of rat liver microsomes].

Administration to white rats of phenobarbital, benzonal and benzobamyl in a dose of 1/20 LD50 for 4--14 days was accompanied by enhanced respiration of microsomes isolated from liver tissue, by acceleration of NAD.H and NADP.H oxidation, by activation of amidopyrin and hexobarbital metabolism, by an increase in the hepatocyte content of P 450 and b5.

[Intragroup serotyping of meningococci].

The method for obtaining antisera to meningococci of different serotypes are described and the scheme for the preparation of serotyping is presented, as well as the method for the preparation of the determinate fraction of serotype 2. Antisera to typing antigens 1, 2, 2-7, 2-10, 4, 5, 6, 8 (1) have been obtained, their specificity tested in parallel experiments with American and French typing sera. When typing meningococci, the use of antisera to purified protein antigen 2 is recommended.

[Study of Vibrio cholerae fractions. III. The gel chromatographic and immunochemical characteristics of vibrionin].

Three fractions differing by molecular weight (fraction 1--greater than 14 x 10(4)--less than 2 x 10(6) dalton, fraction 11--greater than 7 x 10(4)--less than 14 x 10(4) dalton, fraction III--greater than l0(4) less 35 x 10(3) dalton) were isolated from vibrionin, a preparation of cholera allergen, by gel chromatography on Sephadex G-150. These fractions possessed a different immunochemical activity and were capable of detecting the state of increased sensitivity to cholera vibrio.

[Study of Vibrio cholerae fractions. II. A comparison of the immunochemical and biological properties of preparations of alkaline extracts (vibrionin) and the endotoxin of Vibrio cholerae].

Comparative study of immuno-chemical and biological properties of vibrionin and cholera vibrio endotoxin showed vibrionin to contain up to 73.5% of protein, whereas endotoxin preparations contained only 25% of protein. Use of disc electrophoresis in polyacrylamide gel permitted to determine the relative electrophoretic mobility of the protein component bands of both preparations.

[Protoplast fragmentation as one of the possible mechanisms of L-transformation of bacteria (Clostridium perfringens)].

As revealed, the action of lysozyme on the cells of Clostridium perfringens BP-6K led to the formation of not only typical spheroplasts, but also of cells whose peripheral parts of the cytoplasm were fragmented by membrane component. Small bodies framed by the membrane proper and containing granular and fibrillar components were formed. They were polymorphic in osmium treatment, and had smooth contours in preliminary use of aldehyde fixation.

[Cholera vibrio fractions. I. Isolation of alkaline extracts of cholera vibrios and a study of their biological properties].

The authors suggest a well-reproducible method of preparation of a biologically-active fraction of an alkaline extract of cholera vibrio from the strain 569B of Inaba serological type consisting in a preliminary triple removal of endotoxin (O-antigen) of the causative agent from the microbial cell suspension with trichloracetic acid with the subsequent extraction of the microbial mass and neutralization of the extract obtained capable of effective detection of the state of specific sensitization of the organism to cholera vibrio. Preparations of the alkaline extracts (cholera allergen) obtained were standard by chemical composition, they were characterized by a high protein content (up to 77%) and were practically nontoxic. These preparations can be used as a bacterial allergen for the experimental investigations.

[Electrophoretic characteristics of lactate dehydrogenase isoenzymes of lactobacilli and determination of their activity].

Lactate dehydrogenase isoenzymes of 10 museum strains of lactobacilli were examined electrophoretically in polyacrylamide gel. With respect to the relative electrophoretic motility and enzymic activity certain differences in the isoenzyme spectra of lactate dehydrogenase can give additional evidence for the identification of lactobacillar species.