[Cloning the Staphylococcus aureus enterotoxin B gene, obtained by polymerase chain reaction, and its expression in Escherichia coli cells].

To determine the Staphylococcus aureus enterotoxigenicity, we have developed an approach based on polymerase chain reaction (PCR). Using this method several S. aureus strains have been screened for the presence of the enterotoxin B gene.

[The use of monoclonal antibodies for determining the transferrin content of the urine].

The authors used monoclonal antibodies to human transferrin for the development of a high-sensitive test system for the assessment of transferrin levels in urine with enzyme immunoassay. The conditions of the immunoassay performance were optimized. The sensitivity of the test system enabled the authors to define the levels of transferrin up to 1 ng/ml.

[The characteristics of proteinuria during intensive physical loading].

Intensive physical exercise result in certain changes in the body, kidneys in particular. A total of 23 highly qualified sportsmen were investigated for plasma proteins in the urine before and after the graded bicycle ergometry. The impairment of renal reabsorption due to physical load was evidenced by significant amounts of albumin in the afterload urinalyses of all the examinees, and the appearance of IgG in urinalysis of 6 sportsmen in comparison to the urinalysis before the exercise which reported insignificant levels of albumin in 6 subjects only.

[The differential diagnosis of allergic prostatitis].

The leading role of allergic components was established in some rare patterns of prostatitis. To develop the methods applicable for differential diagnosis of allergic prostatitis, the authors followed up 60 patients aged from 15 to 53 years who suffered from chronic prostatitis and 10 normal controls. The patients with bacterial disease, abacterial prostatitis with concurrent allergic components or subjects with allergic prostatitis were enrolled in the study.

[The early stages of chronic kidney failure in children with congenital obstructive uropathies and results of surgical treatment].

Ninety-six children, aged 4 to 14, with congenital obstructive uropathies were subjected to 131I-hippuran renography, followed by mathematical processing of renographic curves, the measurement of blood mean molecular levels, and blood and urinary immunochemical tests before, and 1 month as well as 1 year after surgery. Intraoperative renal biopsy was taken from 20 patients with unilateral hydronephrosis. Latent chronic renal failure (CRF) was identified where a deficiency of total renal clearance of 131I-hippuran (20 to 56%) was combined with a rise in blood mean molecules from 0.