Use of ImageJ as an image processing method for the assessment of post-surgical bruises.

Purpose: To develop, through ImageJ, an automated, non-invasive, objective method to analyze images of bruises that allowed quantifying variation in its size overtime for its later use in clinical trials.

Methods: The ImageJ software was used to create automated macros that were executed on 38 images of the untreated eyes of 19 patients that participated in a post-marketing, randomized, controlled clinical trial that assessed the efficacy of a cream to reduce post-blepharoplasty minor hematomas. Three image processing systems were used with the macros created with ImageJ: RGB, RGB2, and HSB.