The effect of kisspeptin on the functional activity of peripheral blood monocytes and neutrophils in the context of physiological pregnancy.

Hypothalamic hormone kisspeptin is also produced by placental syncytiotrophoblast. Blood leukocytes express a specific membrane receptor of kisspeptin (KISS-1R). Since kisspeptin-54 enters the bloodstream during pregnancy, the hormone has a systemic effect on the leukocytes only in this period.

Role of Kisspeptin in Regulation of Reproductive and Immune Reactions.

The work is focused on physiological role of the hormone kisspeptin produced by neurons of the hypothalamus anterior zone, which is a key regulator of reproduction processes. Role of the hormone in transmission of information on metabolic activity and induction of the secretion of gonadotropin-releasing hormone (GnRH) by the hypothalamus that determines gestation processes involving fertilization, placentation, fetal development, and child birth is considered. The literature data on molecular mechanisms and effects of kisspeptin on reproductive system including puberty initiation are summarized and analyzed.

Prevalence of postpartum endometritis and antimicrobial resistance of responsible pathogens in ukraine: results a multicenter study (2015-2017).

Objective: The aim: To obtain the prevalence of postpartum endometritis women and antimicrobial resistance of responsible pathogens in Ukraine.

Patients And Methods: Materials and methods: We performed a retrospective multicenter cohort study. The study population consisted of all women who had a vaginal delivery or cesarean section in 14 Regional Women's Hospitals of Ukraine.

[A NEW LOOK AT GILBERT SYNDROME (LITERATURE REVIEW)].

To illuminate modern ideas about Gilbert's syndrome (GS). GS is a common familial hyperbilirubinemia that can reduce the risk of various age-related diseases due to the antioxidant properties of bilirubin. In this case, slightly elevated unconjugated bilirubin in GS is strongly associated with a "reduced" prevalence of chronic diseases, in particular cardiovascular disease (CVD), as well as CVD-related and all-cause mortality.

Effect of Estriol, Chorionic Gonadotropin, and Oncostatin M on the Expression of Recombinase RAG-1 in Regulatory T Lymphocyte Subpopulations.

We studied the effect of estriol, chorionic gonadotropin, oncostatin M, and hormone-cytokine combinations on the expression of recombinase RAG-1 in T-regulatory (Treg) and T helper 17 (Th17) lymphocytes. It was found that estriol in a concentration corresponding to the first trimester of pregnancy increased the level of Treg (CD4FoxP3) cells and suppressed the formation of Th17 (CD4RORC) lymphocytes. This effect was nor observed after individual administration of chorionic gonadotropin and oncostatin M, but some combinations of the studied hormones with oncostatin M increased the percentage of CD4FOXP3 cells.

Regulation of Recombinase Rag-1 Expression by Female Sex Steroids in Treg and Th17 Lymphocytes: Role of Oncostatin M.

The effect of estradiol (E), progesterone (P), and oncostatin M (OSM) on the differentiation of CD4 T cells to T regulatory (Treg) lymphocytes and T helpers 17 (Th17) was investigated. The possibility of revision of the T cell receptor in these subpopulations by evaluating the expression of RAG-1 recombinase was also studied. E at concentrations characteristic of pregnancy trimester I, but no P or OSM, increased the Treg level.

[The motivation of participants of the "Zemstvo doctor" program in the Tyumen oblast and evaluation of their satisfaction with conditions of work and life in rural territory].

The article presents the results of the second stage of the study of the motivation of participants of the "Zemstvo Doctor" program, working in medical organizations in rural municipal districts of the Tyumen region (without okrugs) and the evaluation of their satisfaction with labor and living conditions. The sociological questionnaire survey was carried out in 2016-2017 with purpose of evaluating effectiveness of the "Zemstvo Doctor" program and the risks of medical personnel support of rural health care system. During the first stage of study, participants of the program in Tyumen municipal district were interviewed and the results were published.

[THE LEVEL OF PROTECTION FROM OXIDATIVE STRESS AND THREE-YEAR DYNAMICS OF STRUCTURAL AND FUNCTIONAL CHANGES OF THE MYOCARDIUM IN CHRONIC ISCHEMIC HEART DISEASE IN MEN].

Aim - to assess the nature and three-year dynamics of structural and functional changes in the cardiovascular system in relation to the levels of enzymatic and non-enzymatic antioxidants in chronic coronary heart disease in men. The study included 246 men aged 48 to 65 years (mean age 58 years (53,0; 63,0)) with stable form of ischemic heart disease - angina FC II-III CHF I-III FC (NYHA), observed during 2012-2015. To assess the level of oxidative stress in all patients by ELISA was determined by lipid peroxide, total antioxidant status (CCA) and levels of non-enzymatic antioxidant bilirubin (BR).

Hormonal Regulation of Dendritic Cell Differentiation in the Thymus.

We studied the effect of hormones estriol, ghrelin, kisspeptin, and chorionic gonadotropin in concentrations corresponding to their content in the peripheral blood in each trimester of pregnancy on the expression of membrane molecules on myeloid and plasmacytoid dendritic cells of the thymus. It was found that thymic myeloid dendritic cells are sensitive to the action of estriol and kisspeptin. Estriol in a concentration of the first trimester of pregnancy reduces the number of myeloid dendritic cells expressing receptor for thymic stromal lymphopoietin (CD11c+TSLP-R) and inhibitory molecule B7-H3 (CD11cCD276).

MicroRNA in hormonal mechanisms of regulation of NK cell function.

We investigated the effect of human chorionic gonadotropin, estriol, leptin, ghrelin, and kisspeptin on the microRNA expression in separated NK cells. All of these hormones are able to effectively modulate the expression of microRNAs both stimulating and suppressing the cytotoxic potential of NK cells and thereby indirectly regulate the functions of these lymphocytes.

Hormonal regulation of NK cell cytotoxic activity.

The effects of chorionic gonadotropin, estriol (E), leptin, ghrelin, and kisspeptin on the intracellular expression of perforin, granzyme A, and granzyme B was studied in separated NK cells. All studied hormones except E are could modulate the expression of cytotoxic enzymes in NK cells by suppression of the expression of the most active proapoptotic agents, resulting in increased expression of granzyme A, which is typical of the decidual subpopulation of these lymphocytes.

Roles of leptin and ghrelin in the regulation of the phenotype and cytokine production by NK cells from peripheral blood.

Both leptin and ghrelin used separately at the concentrations corresponding to trimesters II-III of pregnancy increase the number of CD56 NK cells in mononuclear cell suspension; their combination also enhances the L-selectin expression on the surface of these cells in the culture. These hormones do not affect the production of TGF-β1, IL-17А, or IFN-γ by NK cells, and they inhibit the production of IL-10. Leptin decreses the IL-4 production by NKp46 cells, but the presence of ghrelin abrogates this effect.

[The Influence of Chorionic Gonadotropin and Estriol on NK Cells Phenotype and Functional Activity.].

The influence of chorionic gonadotropin (CG) and estriol (E3) at concentrations typical of pregnancy on the expression of phenotypic markers and cytokine production by separated NK cells were studied. It is found that these hormones increase the percentage of CD56brightL-selectin+ NK-cells, but also stimulate the expression of the inhibitory molecule NKG2A in the lymphocytes. In addition, E3 and CG stimulate the production of TGF-B, inhibiting the secretion of all other cytokines by separated NK cells.

The effect of kisspeptin on the functional characteristics of isolated NK cells.

The effect of kisspeptin at concentrations typical of pregnancy on the functional activity of isolated cytokine-primed NK cells has been investigated. The hormone has been shown to promote an increase in the proportion of CD56(bright) NK cells, as well as an increase in the L-selectin expression on the cell surface. Assessment of cytokine levels has shown that kisspeptin suppresses the production of IL-4, IL-10, and IFN-γ while stimulating the production of TGF-β by isolated NK cells.

[Microbiological Aspects of Radioactive Waste Storage].

The article gives information about the microorganisms inhabiting in surface storages of solid radioactive waste and deep disposal sites of liquid radioactive waste. It was shown that intensification of microbial processes can lead to significant changes in the chemical composition and physical state of the radioactive waste. It was concluded that the biogeochemical processes can have both a positive effect on the safety of radioactive waste storages (immobilization of RW macrocomponents, a decreased migration ability of radionuclides) and a negative one (biogenic gas production in subterranean formations and destruction of cement matrix).

Role of CD40-dependent signal in induction of recombinase RAG-1 expression in peripheral T cells of patients with autoimmune diabetes mellitus.

We studied the mechanisms of induction of recombinase activity in peripheral T cells of patients with autoimmune type 1 diabetes mellitus. It was shown that the presence of CD40 on T cell membrane (not typical of these cells) is crucial for this process: expression of recombinase RAG-1 in diabetic patients was detected primarily in αβTCR(+)CD40(+) lymphocytes; targeted CD40-dependent activation of intact T cells in vitro increases, while blockade of CD40 signal in the culture of stimulated T cells abolishes recombinase expression.

[Evaluation of the efficiency of Angara River water protection measures against pollution by petroleum products].

The process installations and storage reservoirs of a petroleum refinery have leaks of petroleum products (PP) that pollute soil, underground waters, and eventually nearest water objects, by worsening their hygienic state. Environmental and economic assessments of the Angara River water protection system that is in operation at the petroleum refinery OAO "Angara Petroleum Company", which comprises well clusters, a gravel-filled trench, and a drainage system, have shown the high values of preventable relative natural and economic damages and other economic indicators. At the same time, comparison of the amount of PPs accumulated at the industrial site with their annual withdrawal has demonstrated a need for further development of a river protection system.

[Hygienic standardization of fluogermanate in the air of a working area].

The paper presents the data on the impact of the most possible concentrations of fluogermanate (GeF4) in the air of a working area with a pencil-beam irritant acting the respiratory organs and those obtained from chronic experiments. The most possible concentration is 0.2 mg/m3 (Class II hazard).

[Various mechanisms of the direct and indirect effects of chorionic gonadotropin on the phagocytic activity of leukocytes].

The effects of chorionic gonadotropin (CG) and CG-induced hormonal background on the phagocytic activity of peripheral blood leukocytes and peritoneal macrophages were studied in female Swiss mice. In vitro, the direct immunosuppressing effect of CG on phagocytes depended on the function of L-type Ca2+ channels and was increased by an additional paracrine regulation by prostaglandins. At the systemic level, the CG effects depended on the activated production of sex steroids, which abolished the phagocyte-suppressing effect of CG and the development of adaptive immune responses.

[Symptoms of gastroesophageal reflux and quality of life: population study].

Aim: To study prevalence of symptoms of gastroesophageal reflux (GER) in a free living population and to evaluate quality of life (QOL) in this disease.

Material And Methods: The gastroenterological symptoms and QOL were studied in a representative sample of adult population of 495 males and 548 females aged 45-70 years using a validated questionnaire, SF-36 questionnaire.

Results: GER symptoms were seen in 17.

[Chorionic gonadotropin regulation of leukocytic phagocytic activity in the formation of a primary humoral immune response].

Experiments on male Swiss mice studied the effect of chorionic gonadotropin (CG) on the phagocytic activity of peripheral blood leukocytes and peritoneal macrophages, by taking into account the stages of formation of a humoral immune response. The administration of CG was found to stimulate the splenic production of antibody-forming cells (AFC) and to elevate the serum level of endogenous progesterone (Pr) in the mice. Irrespective of the dose, CG suppressed the phagocytic activity of peripheral blood neutrophils and peritoneal macrophages in the in vitro system.