Publications by authors named "Gorban L"

Aim: The research was aimed on analysis of the remote consequences of Chornobyl accident on the reproductive function of men adult residing in Ukraine.

Materials And Methods: 232 male volunteers with mean age of 34 years (range 20-47) from 5 different regions of Ukraine (Zhytomyr, Ivano-Frankivsk, Kyiv, Poltava, and Kyiv city) were enrolled in cross-sectional studies of long term radiation effects on seminal plasma and sperm. All manipulations, analysis and classification of ejaculates were done accordingly to WHO recommendations.

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Objective: To study the influence of long-term gamma-irradiation with low dose rate (13×10-6 cGy/s) on the body, testes and ventral prostate, development of laboratory rats, as well as quantitative parameters of sperm production along with kinetic characteristics of spermatozoa.

Materials And Methods: The experiments were performed on laboratory white rats of 2,5 months in age. Animals were irradiated in gamma-field of "Ethalon" device in a dose range 0.

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Objective: To study the impact of X-irradiation on the morpho-functional characteristics of rabbit spermatozoa and the contents of fructose, L-carnitine, α-tocopherol, ascorbate and citrate in the animal sperm.

Materials And Methods: The total body irradiation of rabbits (Soviet Shinchilla) was performed at the RUM-17 device in the dose range of 1.0-7.

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Formation of single- and double strand breaks in DNA which may be discovered by microelectrophoresis in agarose gel is one of the criterion of genetical lesions in cells as a result of apoptosis or of genotoxic agent action. Genotoxic action of nickel chloride at the level of DNA of the individual cells in the initial culture of human embryonic haemopoietic cells was studied. It has been shown that about 2% cells in the studied in vitro populations were in the apoptosis state.

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In November 2000, an outbreak of Giardiasis intestinalis with aqueous transmission was recorded in one of the microdistricts of Perm. Thirty six children aged 1-7 years fell ill, 2 parasitic carriers were identified among the staff. The diagnosis was parasitologically verified in all children with clinical manifestations.

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We assessed the risk for human tick-borne encephalitis (TBE), ixodid tick-borne borrelioses, and double infection from 1994 to 1998 in Perm, which has among the highest rates of reported cases in Russia. We studied 3,473 unfed adult Ixodes persulcatus ticks collected from vegetation in natural foci and 62,816 ticks removed from humans. TBE virus and Borrelia may coexist in ticks.

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A special epidemiological experiment to prevent borreliosis in persons bitten by infected ticks was performed in 1992-1994 in the Russia's Perm region where Borrelia garinii and B.afzelii circulate, and Ixodes persulcatus tick is the sole vector transmitting these pathogens to human beings. Adult ticks were removed from the bodies of persons who had referred to health facilities for first aid.

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A study was made of the labour conditions of those workers engaged in the production of basalt fibre (BF). Morphological makeup is examined as is dispersity and cytotoxicity of the dust produced in the process of BF making. An issue is addressed of usefulness of setting special hygienic regulations for BF dust.

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A direct microscopic analysis of fixed smears and live preparations was used to reveal whether spirochetes are present in the gut and salivary glands of adult Ixodes persulcatus ticks spontaneously infected with Borrelia garinii and B. afzelii. Unfed ticks collected from a vegetation, partially fed ticks removed from human bodies, and ticks deliberately fed on laboratory animals were studied.

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It was found that "Kemper" devices absorb efficiently noxious substances released during arc welding of metals. Conditions for maximum efficiency of the "Kemper" devices are described.

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The article reveals the current issues pertaining to the improvement of control after hazardous substances contents in the welding working zone air. The welding aerosols are multicomponent and constitute a group of complex gas-aerosol mixtures,--the feature which requires a separate detection of the individual concentrations of the most hazardous components, both hard and gaseous. Data on the specific excretion of hazardous substances should be included in respective technical documentation relating to welding processes and materials.

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The possibility of contaminating the breathing air zone with hazardous substances in manual and semi-automated welding increases with the intensity of their formation in the arc zone. This necessitates more comprehensive data on both the specific excreta and the formation intensity of the most hazardous substances, such as hard and gaseous compounds of welding aerosols, with due account of the welding regimen used and electrode diameter, wires, etc. When calculating the effectiveness of both local and general ventilation systems in welding shops, a possibility of a specific one-way hazardous action of some chemical substances (e.

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Chronic experiments on animals showed that aerosols formed during cast iron welding and facing due to the use of electrode nickel-based materials could produce blastomogenic effect that was mostly caused by the presence of copper-and manganese-containing aerosols in the particles of the solid component. Morphologic inhomogeneity of the particles of the solid component, qualitative composition and solubility of the compounds spatially arranged in the surface layer were of great importance for their carcinogenic and toxic effect. The capacity of copper- and manganese-containing compounds to suppress nickel carcinogenic effect should be taken into account when developing welding and facing materials with better hygienic characteristics.

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It was established as a result of cytotoxicity studies of 15 welding dusts of different compositions in experiments on embryonal fibroblast cell cultures that the biologic activity of these substances depends on the solubility in growth media of their major components: alkaline and alkaline earth metals, iron, cremnium and, to a vast degree, that of the manganese. Conclusion has been made that a comparative assessment of welding dusts' biological activity on cell cultures should involve the kinetic parameters of their solubility in growth and supportive media, and the duration of the substances' affecting the cell cultures should be long enough to reveal the combined action of its major ingredients.

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Significant correlation of genetic distances (estimated for 17 polymorphic loci) between populations of Asia and Alaska Eskimos, coast and reindeer chuckchies, Kamchatka koryaks and Chuckotka even with geographic distances (r = 0.650; P less than 0.01) is shown.

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