APPLICATION OF QUERCETIN FOR CORRECTION OF THE IMPAIRMENT OF THE FUNCTIONAL STATE OF THE ENDOTHELIUS OF VESSELS (CLINICAL AND EXPERIMENTAL STUDY).

Objective: in the experiment, to investigate the effect of Quercetin on the NO-dependent reactions of isolated vessels involving endothelium and perivascular adipose tissue (PVAT) after a single X-ray irradiation of rats at a sublethal dose. In a clinical study, to investigate the effect of long-term use of Quercetin on the functional state of themicrovascular endothelium in the elderly patients with metabolic syndrome (MS).

Material And Methods: Experimental studies were performed on vascular fragments obtained from adult male rats(7-8 months) of the control group, in animals exposed to a single R-irradiation at a dose of 7 Gy and animals irradiated in the same dose, which received Quercetin orally for 14 days three times a week based on 10 mg/kg bodyweight.

[THE CLINICAL SIGNIFICANCE OF ENDOSCOPIC MANOMETROGRAPHY OF UPPER GASTROINTESTINAL TRACT IN PATIENTS WITH CHRONIC PANCREATITIS].

The Purpose Of The Research: To study the cavitary esophagogastroduodenal pressure in patients with chronic pancreatitis (CP) depending on the dominant clinical symptoms--gastroesophageal reflux (GER) and abdominal pancreatic pain.

Material And Methods: By esophagogastroduodenoscopy that included manometrography and acid-perfusion test--were examined 86 patients with CP and 25 healthy individuals.

Results: 63% of patients with CP detected changes of the esophagus and the lower esophageal sphincter (LES) based on the endoscopy, or manometrography, or test with 0.

[Effect of Korargin on reactivity of isolation aorta of adult and old irradiated rats].

Introduction to the diet of adult (7-8 months) and old (24-26 months) male rats for 30 days after a single R-irradiation at a dose of 5 Gy of preparation "Korargin" (whose active ingredients are L-arginine, contributing to the prevention of endothelial dysfunction, and inosine) in old irradiated rats significantly increased the relaxation of isolated segments of thoracic aorta by the action of acetylcholine, sodium nitroprusside and insulin. In adults irradiated animals this effect was not observed. Vasoconstriction response of isolated segments of thoracic aorta to norepinephrine was decreased in isolated segments of thoracic aorta by adults and old irradiated rats compared with controls, and application of korargin had no significant effect.

[Effect of preparation "Korargin" on metabolic syndrome manifestations in adult and old rats exposed to x-ray irradiation].

Introduction to the diet of adult and old rats for 30 days after R-irradiation at a dose of 5 Gy drug "Korargin" prevented the development of some manifestations of radioinduced metabolic syndrome (MS) in animals of both age groups: in adult irradiated animals--a tendency to decrease insulin levels in blood plasma, increase of cholesterol levels in liver tissue, significantly increased serum high density lipoprotein as compared both to controls and irradiated animals, prevented the decrease in the levels of anions NO2- and NO3- in tissue of the aorta and anions NO2- in tissue of the heart; in old irradiated animals--prevented tended to increase of body weight, the increase in insulinresistance (index HOMA), the decrease in the level of anions NO2- in the aorta tissue, increased levels of anions NO2- and NO3- in the heart tissue compared to controls. Prevention through drug "Korargin" some manifestations of radioinduced MS in adult and old animals, indicates the prospects of exploring the possibility of correction of the violations of carbohydrate and lipid metabolism caused by exposure to ionizing radiation, in individuals of both age groups.

[Biomechanical rationale of the tense screw-pin osteosynthesis of proximal humerus fractures].

This article developed the question of biomechanical studies of new low-impact method of surgical treatment of fractures of the proximal humerus. Designed latch consists of standard needles with a diameter 2 mm cortical screws with diameter 4.5 mm and fixing washers original design.

[Psychiatric care act of Ukraine and issues concerning reformation of the mental health protection service].

An analysis was performed of the conception and content of a new Psychiatric Care Act by making a comparison with data from published literature and the present-day status of the mental health protection service. The main features of the crisis of psychiatry in Ukraine are characterized together with possible ways of resolving it. Main trends in reformation of the psychiatric service are identified that are to be secured by relevant acts of departmental and interdepartmental character based on law.

[Kirlian diagnostics in a theraputic practice].

118 adult patients with respiratory, cardiovascular, digestive, urino-genital or endocrinological diseases have been observed. Kirlian diagnostics on palm finger radiation has been used along with common clinico-laboratory methods. Data comparison of these methods showed that Kirlian diagnostics can be used in clinical differential diagnostics as an supplementary method to determine the severity of the pathological process, its pathogenesis, functional state of compensatory reactions.

[Assessment of escalation of AIDS and HIV infection in Ukraine. Information technology for management of epidemics and risk ranking].

The article presents an approach to the development of information technology for management of epidemics and risk ranking to increase the efficacy of struggle with HIV/AIDS in Ukraine, minimize incidence rate, maximize the quality of health care, and augment the reliability of a long-term forecasting of epidemiological situation in Ukraine and its separate regions.

[Spirulina: perspectives of the application in medicine].

The data on biologically active components included in structure of blue-green seaweed spirulina, determining its value as food additive and medicinal means are submitted. The results of use of spirulina in experimental models of pathology and clinical practice are generalized.

[Managing epidemic risk in the Ukraine: new approaches and development strategies].

A concept is presented of development of the epidemiological service in Ukraine. It is based on modern informative computer technologies of epidemic risk management and epidemic situation assessment. On the basis of mathematical modelling and theory of optimal control a new approach has been worked out for epidemic risk estimation and forecasting of epidemic situation development.

[Clinical and experimental study of spirulina efficacy in chronic diffuse liver diseases].

The results of examination of 60 patients presenting with chronic diffuse disorders of the liver and seventy experimental animals with toxic affection of the liver, having been administered spirulina treatments, suggest clinical-and-laboratory effectiveness of this drug. The hepatoprotective properties of spirulina are referrable to its antiinflammatory, antioxidant, membrane-stabilizing, and immunocorrecting actions. In this way the employment of spirulina is believed to be pathogenetically validated in chronic diffuse liver conditions, permitting stabilizing the process and preventing the transformation of chronic hepatitis into hepatocirrhosis.

[The role of immunological mechanisms in the development of the late sequelae of nuclear disasters].

Effects, both specific and non-specific, were analysed of ionizing radiation in a comparative evaluation of medical sequels of the Chernobyl disaster and of the Semipalatinsk Range nuclear test. A hypothesis is advanced assuming that development of all major bodily morbid conditions is directly related to the dysfunction of certain complex of organs, the thyroid gland in the first place, the thymus gland known to be a central organ of systemic immunogenesis. A breach in the thymus link triggers harmful cascade reactions of neuroimmunoendocrinological regulation, appeared to be responsible for the pathogenesis of practically all pathologies disclosed.

[The concept of constructing a uniform state information system on transplantation in Ukraine].

A conception is submitted of setting-up a state information system of transplantation in Ukraine (Ukrtransplant) integrated into systems of transplantation of other countries (the European Bank of Transplants-Eurotransplant-included). The information system Ukrtransplant is designed for automatization of processes of taking, genetic and tissue typing, reliable and safe preservation of organs and tissues, delivery of data from the State Register (the bank of data on graft donors and recipients), processing of information about availability of and demand for organs and transplantations performed, and for speedy providing all forms and activities of the state system Ukrtransplant with valid information incident to managerial/administrative matters.

Invertors mechanism of the influence of insulin on the condition of plasma membranes of myocardiocytes in animals of different age.

Effect of insulin on Na, K-ATPase activity and phospholipid (PL) composition of myocardiocytes' plasma membrane (PM) was investigated in adult (6 months old) and old (28 months old) male Wistar rats. Administration of insulin increases Na, K-ATPase activity and phosphatidilethanolamin content, and decreases phosphatidilinositol content of myocardiocytes' PM in adult animals. Administration of insulin doesn't change Na, K-ATPase activity and decreases phosphatidilethanolamin content of myocardiocytes' PM in old rats.

[Age-related features of the effects of alpha- and beta-adrenomimetics on the glucocorticoid function of the adrenal glands].

In vitro effect of alpha-adrenomimetic naphtizin (0.1 mM X 1(-1) and beta-adrenomimetic isoproterenol (10 mcM X 1(-1) on the basal and ACTH-stimulated (10 U X 1(-1) production of II-oxoketosteroids (II-OKS) by isolated adrenals (IA) was studied in adult (6 mo) and old (28 mo) male rats. Naphtizin increased ACTH-stimulated secretion of II-OKS by IA of adult rats, but had no effect on the above secretion of old rats.

[Effect of the antioxidant dibunol on the hormonal status of adult and old rats].

The effect of chronic administration of antioxidant dibunol (BHT) on the blood level of thyrotropin, corticotropin, thyroxin, triiodothyronine, 11-hydroxycorticosteroids, immunoreactive insulin and the reactivity of isolated thyroids and adrenals to tropic hormones were studied in adult and old male rats. Chronic administration of BHT was found to inhibit the thyroid functional activity of both animal groups and to reduce the blood insulin level in the old rats. The results obtained suggest that hormonal effects play an essential role in the mechanism of BHT action on the organism.

[Effect of Ca2+- and K+-channel blockers on ACTH-stimulated steroidogenesis of isolated adrenal glands of adult and old rats].

Ca2+ channel blocker verapamil (0.2 mM) and K+ channel blocker 2-aminopyridine (1.0 mM; 5.

Peculiarities of neural regulation of the thyroid, adrenocortical and testicular functions in old age.

The age changes of neural control over the function of the thyroid, adrenal cortex and testicles were examined in adult (6 months) and old (28 months) male rats. In old age, there was a weakening of adrenergic control of thyroidogenesis, alpha-adrenergic and M-cholinergic control of glucocorticoid function of the adrenal cortex and reduction of adrenergic and M-cholinergic influences in the regulation of steroidogenic function of the testicles.

[Effect of alpha- and beta-adrenoreceptor blockaders on thyroid function in rats of various ages].

The effect of alpha-adrenoblocker dibenamine and beta-adrenoblocker propranolol on the blood level of thyroid hormones (TH) and TH production by isolated thyroids (T) was investigated in adult and old male rats. Isolated T were incubated for 4 h in medium 199 with or without TSH (100 mU/ml). TH blood concentration and specific TH production by isolated T was decreased with age.

Membrane potential of cells and its regulation during aging. 2. Report: the effect of hormones on the level of the cellular plasma membrane polarization.

Age-dependent changes in the polarization of plasma membranes (PM) of various cell types and the mechanisms responsible for its regulation were studied in the experiments on the adult (6-8 and old (28-32 months) Wistar male rats. It was found that the effect of the hormones on the PM polarization level is altered during aging. This being related to shifts in the number and affinity of the hormonal receptors, energetic processes and protein synthesis in the cell.

Membrane potential of cells and its regulation during aging. 1. Report: the role of energetic metabolism and plasma membrane phospholipid contents.

Age-dependent changes in the polarization of the plasma membranes (PM) of various cell types and the mechanisms responsible for its regulation were studied in the experiments on the adult (6-8 months) and old (28-32 months) Wistar male rats. The relationship was found between the specificity of cellular function and the pattern of changes in resting potential (RP) and action potential (AP). In senility, changes occur in the ratio of different pathways of energy provision of the ionic transport: the contribution made by enzymic reactions to the mechanism of maintenance of the PM polarization level is diminished.