Development and Validation of PCR Diagnostic Assays for Detection of and .

PCR is the most effective method for detecting difficult-to-cultivate pathogens and pathogens that are part of mixed infections in animals, such as , which causes bird ornithobacteriosis, or , which causes infectious coryza. In this work, we developed and validated two efficient and sensitive diagnostic assays for the rapid and accurate detection of and DNA in bacterial isolates and clinical samples using real-time PCR with TaqMan-like probes. When designing the PCR assays, we performed in silico analysis, optimized DNA isolation methods and PCR conditions, and assessed the analytical and diagnostic performance of PCR.

Neurodynamic approaches for multi-agent distributed optimization.

This paper considers a class of multi-agent distributed convex optimization with a common set of constraints and provides several continuous-time neurodynamic approaches. In problem transformation, l and l penalty methods are used respectively to cast the linear consensus constraint into the objective function, which avoids introducing auxiliary variables and only involves information exchange among primal variables in the process of solving the problem. For nonsmooth cost functions, two differential inclusions with projection operator are proposed.

Approximating Nash equilibrium for anti-UAV jamming Markov game using a novel event-triggered multi-agent reinforcement learning.

In the downlink communication, it is currently challenging for ground users to cope with the uncertain interference from aerial intelligent jammers. The cooperation and competition between ground users and unmanned aerial vehicle (UAV) jammers leads to a Markov game problem of anti-UAV jamming. Therefore, a model-free method is adopted based on multi-agent reinforcement learning (MARL) to handle the Markov game.

[Oxytocin genetic pathway (CD38) and SDQ psychosocial characteristics in adolescent schoolchildren].

Objective: To determine an effect of CD38 on the regulation of oxytocin (OT) production and to identify psychosocial characteristics according to the Strength and Difficulties Questionnaire (SDQ) in adolescent schoolchildren.

Material And Methods: The study included 308 adolescents, aged 11-18 years, of an unbiased school sample from three large cities of Central Siberia (Krasnoyarsk, =194; Abakan, =76; Kyzyl, =38). All participants were Russian.

Multi-agent based optimal equilibrium selection with resilience constraints for traffic flow.

Traffic guidance and traffic control are effective means to alleviate traffic problems. Formulating effective traffic guidance measures can improve the utilization rate of road resources and optimize the performance of the entire traffic network. Assuming that the traffic coordinator can capture traffic flow information in real-time utilizing sensors installed on each road, we consider the strong resilience constraints to construct an optimal selection problem of balanced flow in the traffic network.

Generalized and Specific Problematic Internet Use in Central Siberia Adolescents: A School-Based Study of Prevalence, Age-Sex Depending Content Structure, and Comorbidity with Psychosocial Problems.

Unlabelled: We aimed to assess the prevalence, content structure and, psychological comorbidity of PIU in Russian adolescents. In addition, the design of our research provided an opportunity to compare demographic and psychological patterns of different forms of PIU: generalized (PIUgen) and specific problematic video game use (PUgame), as well as problematic social media use (PUsocial).

Methods: This is a one-stage cross-sectional observational study of school sampling in three major Siberian cities.

[The relationship between internet addiction and sleep disorders in adolescents of Central Siberia in different types of consumed content].

Objective: To establish the relationship between disturbances of the regime and quality of night sleep and daytime sleepiness in adolescents from Central Siberia with internet addiction with different types of content consumed.

Material And Methods: A single cross-sectional observational study of a school sample in three large cities in Siberia was performed. The study included 4615 schoolchildren aged 12-18 years (46.

Internet Addiction and Sleep Problems among Russian Adolescents: A Field School-Based Study.

Unlabelled: This study aims to establish a link between disturbances in the night sleep habitus, quality of sleep, and daytime sleepiness in adolescents with Internet addiction and different types of content consumed.

Methods: This is a cross-sectional observational study of a school sample in three large cities in Central Siberia. 4615 schoolchildren of 12-18 years old were examined.

Oxytocin Pathway Gene () Variants Are Not Related to Psychosocial Characteristics Defined by Strengths and Difficulties Questionnaire in Adolescents: A Field School-Based Study.

CD38 is a transmembrane glycoprotein that regulates oxytocin (OT) production and influences social interactions. The oxytocin receptor (OXTR) has been studied intensively regarding its association with human psychosocial functions. Many studies have demonstrated a link between rs3796863 and rs53576 polymorphic regions and psychosocial characteristics as well as various psychiatric disorders in adolescents.

[Borderline mental disorders in schoolchildren].

Objective: To study the frequency of borderline mental disorders in children of secondary general education schools.

Material And Methods: We examined 1013 male and female schoolchildren, aged 12-14 and 15-17 years. In the first stage, the they completed a screening questionnaire to estimate the presence of a range of psychosomatic syndromes.

Efficiency of Very Low Doses of Antibodies to S100 Protein in the Complex Therapy of Functional Dyspepsia in Children.

We showed the data on clinical efficiency of low doses of antibodies to brain-specific protein S100 in the complex therapy of schoolchildren with painful variant of functional dyspepsia syndrome. Significant positive effects of the substance on the dynamics of epigastric pain, in particular, its duration, were found. The maximum clinical effect was observed in children with emotional disorders (~1/3 of all examined children).

[The overview of research on toxicological (forensic) chemistry based on the materials of the abstracts of dissertation theses. The forms and modes of information].

The authors discuss the system of information about investigations on toxicological (forensic) chemistry carried out for obtaining the scientific degree as it has formed in this country. They present a review and a list of theses and their abstracts in this discipline defended during the period from 1936 to 2010. The analysis of the themes of the studies is performed with reference to the dynamics of preparation of the theses in the Soviet and post-Soviet periods.

[On the problem of informational support of scientific research in the field of forensic (toxicological) chemistry].

The present work was designed for the analysis of informational support of scientific research in the field of forensic (toxicological) chemistry in this country during the period preceding the collapse of the Soviet Union and in present-day Russia. The established channels of transmission of relevant information are characterized. Recommendations for the further development of the work in this sphere and its improvement are proposed.

[The structural and functional status of the immune system in pre-school children infected with Mycobacterium tuberculosis].

Sixty children infected with Mycobacterium tuberculosis (MBT), aged 3 to 5 years were followed up in the period before specific chemotherapy. The studies established structural and functional changed in the immune system in young MBT-infected children. Moreover, the disorders found in immune homeostasis were of combined nature.

[Use of thin layer chromatographic analysis in forensic medical analysis of urine for opiates].

The study results of identifying the opium alkaloids (morphine, codeine and their synthetic analogue heroin) in urine during the forensic chemical examinations by using the method of thin-layer chromatography (TLC) are described. Several solvent systems and chromogenic reagents designed for the TLC analysis of opiates are presented. Solvent systems were defined to separate the opiates (isolated from urine) in Sorbphyl plates; besides, a chromogenic reagents' kit was suggested to detect them in the forensic medical analysis of urine.

[The isolation and detection of actellic by thin-layer chromatography in the forensic chemical study of cadaveric organs].

A method for analysis of human cadaveric organs for actellik, an organophosphorus insecticide (pyrimiphos methyl), has been developed, consisting in the agent isolation using hexan, separation of extraction substances by extraction redistribution between hexan and dimethylformamide, and subsequent preparative chromatographic isolation in a sorbent layer (KSK silica gel or silicic acid) with chloroform used as solvent. This method permits isolation of 49-52% of actellic added to the liver. A method of purified extract analysis by thin-layer chromatography on silufol plates and KSK silica gel using a complex of chromogenic reagents and selective solvent system has been worked out.