Langmuir-Blodgett Films with Immobilized Glucose Oxidase Enzyme Molecules for Acoustic Glucose Sensor Application.

In this work, a sensitive coating based on Langmuir-Blodgett (LB) films containing monolayers of 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE) with an immobilized glucose oxidase (GOx) enzyme was created. The immobilization of the enzyme in the LB film occurred during the formation of the monolayer. The effect of the immobilization of GOx enzyme molecules on the surface properties of a Langmuir DPPE monolayer was investigated.

Langmuir-Blodgett Films of Arachidic and Stearic Acids as Sensitive Coatings for Chloroform HF SAW Sensors.

Properties of the Langmuir-Blodgett (LB) films of arachidic and stearic acids, versus the amount of the films' monolayers were studied and applied for chloroform vapor detection with acoustoelectric high-frequency SAW sensors, based on an AT quartz two-port Rayleigh type SAW resonator (414 MHz) and ST-X quartz SAW delay line (157.5 MHz). Using both devices, it was confirmed that the film with 17 monolayers of stearic acid deposited on the surface of the SAW delay line at a surface pressure of 30 mN/m in the solid phase has the best sensitivity towards chloroform vapors, compared with the same films with other numbers of monolayers.

Morphology of Mixed Langmuir and Langmuir-Schaefer Monolayers with Covered CdSe/CdS/ZnS Quantum Dots and Arachidic Acid.

The process of formation of a Langmuir-Schaefer (LS) matrix based on a mixed monolayer of arachidic acid (AA) and 8 nm CdSe/CdS/ZnS quantum dots (QDs) stabilized by molecules of trioctylphosphine oxide (TOPO) was investigated. The change in the morphology, monolayer compressibility, and area per elementary cell of the created mixed monolayers, depending on the ratio of the components, was studied. It is shown that the change in the morphology of Langmuir-Blodgett (LB) monolayers begins to occur at a ratio between the number of QDs and AA molecules of 1:24.

Microstructural Evolution and Phase Formation in 2nd-Generation Refractory-Based High Entropy Alloys.

Refractory-based high entropy alloys (HEAs) of the 2nd-generation type are new intensively-studied materials with a high potential for structural high-temperature applications. This paper presents investigation results on microstructural evolution and phase formation in as-cast and subsequently heat-treated HEAs at various temperature-time regimes. Microstructural examination was performed by means of scanning electron microscopy (SEM) combined with the energy dispersive spectroscopy (EDS) mode of electron probe microanalysis (EPMA) and qualitative X-ray diffraction (XRD).

X-band EPR setup with THz light excitation of Novosibirsk Free Electron Laser: Goals, means, useful extras.

Electron Paramagnetic Resonance (EPR) station at the Novosibirsk Free Electron Laser (NovoFEL) user facility is described. It is based on X-band (∼9 GHz) EPR spectrometer and operates in both Continuous Wave (CW) and Time-Resolved (TR) modes, each allowing detection of either direct or indirect influence of high-power NovoFEL light (THz and mid-IR) on the spin system under study. The optics components including two parabolic mirrors, shutters, optical chopper and multimodal waveguide allow the light of NovoFEL to be directly fed into the EPR resonator.

[The mechanisms of the natural history of small pelvis varicosis].

An analysis was made of the results of an all-round clinical, ultrasound, phlebotonometric and phlebographic examination of 89 patients with the clinical evidence of chronic venous insufficiency (CVI) of the pelvis. It is established that small pelvis varicosis develops because of hemodynamic disorders in the system of the inferior vena cava, iliac and left renal veins. In men, blood reflux from the iliac vein to the parietal tributaries leads to the development of the atypical forms of varicosis.

[Sclerotherapy of small pelvis varicosis].

This paper analyses the results of sclerotherapy provided to 89 patients with small pelvis varicosis. Selection of the treatment technology depended on the clinical variant of disease. In patients with damage to the parietal venous system of the pelvis, the treatment was realized by multiple repeat injections of a sclerosing agent to the pelvic veins in a minimal dose which may induce only proliferative processes in varicose vein walls.

[The diagnosis and treatment of chronic venous insufficiency of the pelvis of non-occlusive genesis].

The clinical, ultrasonic duplex scanning and phlebographic (retrograde pelvis renoovaricography) examinations of 89 patients with chronic venous insufficiency of the pelvis were analyzed. Two forms of the clinical course of the disease were established: parietal and visceral. A detailed clinical, ultrasonic and phlebographic characteristics of these forms are described.

[Age-related changes in metabolism of DNA precursors in patients with mastopathy].

The activity of thymidine kinase, thymidine phosphorylase, adenosine deaminase and 5'-nucleotedase of AMP was studied in tissues, blood serum and lymphocytes of 60 healthy females and 50 females with fibrocavernous mastopathy aged 23-70. It was revealed that age-related changes in the activity of thymidine kinase in blood serum reflect the analogous changes in enzyme activity in tissues of healthy women. A direct correlation was established between thymidine kinase activity and age both in healthy females and those with mastopathy.

[Kinetics of the processes of Streptococcus pneumoniae batch cultivation in relation to the physiological state of the inoculum].

The present investigation, carried out with the use of S. pneumoniae as a model, has shown that the kinetics of cultivation processes depend on the preparation of the seed culture. The use of the seed culture, taken at the end of the exponential phase of growth, leads to high initial and maximum specific growth rates and a shorter lag phase, high productivity of the process and the economic coefficient of the yield of the biomass.

[Dissolved oxygen content in microbiological nutrient media].

The modification of a highly sensitive method for the determination of the concentration of dissolved oxygen in liquid culture media and in water is described. This modification permits the easy conversion of the relative readings of electrode membrane transducers for measuring the partial pressure of oxygen into the readings characterizing the absolute concentration of oxygen in the medium (mg/cu. dm).

[Effect of B group vitamins on dysentery bacteria activity during stab culture].

The addition of the vitamin B complex into the culture medium enhances the productivity of the process ensuring the yield of biomass. The presence of the vitamins of this complex in the culture media intensifies the synthesis of some macromolecular compounds and prolongs the period of the physiological activity of the population.

[Physiological characteristics of pneumococci].

The physiological activity of pneumococci under different physico-chemical conditions of deep cultivation in a liquid culture medium has been studied. The culture has been shown to achieve its maximum physiological activity under the conditions of neutral pHn-7.0) and the minimum content of dissolved oxygen in the medium.

[Dynamics of biologically active enterotoxin release by E. coli].

Accumulation of E. coli enterotoxin in the Finkelshtein's culture medium in growing the cells in a 30-litre reactor was studied. Accumulation of active highly molecular enterotoxin occurred in the course of a 6-hour cultivation of E.

[Method of culturing typhoid microorganisms with automatic regulation of glucose feed].

A system of oxygen-glucose inverse relationship is not acceptable for cultivation of typhoid bacilli with a high reproductive rate and glucose utilization. The elaborated method of automatic glucose supply during typhoid bacilli cultivation permits to lead the process under the optimal conditions by pH and the residual glucose concentration.