Role of radioimmunoscintigraphy and SPET in the diagnosis of patients with malignant melanoma.

The aim of the study was to compare the effectiveness of radioimmunoscintigraphy and single photon emission tomography in the diagnosis of malignant melanoma. Radioimmunoscintigraphy was carried out on 47 patients with stage I to IV malignant melanoma. Seven patients had primary melanoma; the remaining patients had 57 clinically suspected lesions.

[Erythrocyte lipid peroxidation as a diagnostic test in supplying pigs, lambs and chickens with vitamin E].

Experiments with pigs, lambs, sheep, and birds, carried out under various conditions of feeding (with or without supplement or treatment with vitamin E) revealed that the study of the peroxide resistance of erythrocyte lipids could render useful information on the supply of animals with vitamin E. The study of peroxide resistance was shown to be much more accessible than the available chemical methods, and had better reproducibility. This new approach took good consideration of the biologic activity of vitamin E, and was fairly suitable to be employed as a screening diagnostic method.

[Effect of levamisole on the cytochemical function of leukocytes and on blood lysozyme in sheep].

It was established in an experiment with 13 sheep of the local improved breed that the single s/c injection of levamisole at the rate of 7.5 mg per kg of body mass led to a rise of the phagocytic activity of neutrophil leukocytes. These cells also had higher cytochemical activity of the alkaline phosphatase, glucose-6-phosphatase, and lipids (sudanophilia).

[Cytochemical study of acid naphthyl acetate esterase in the lymphocytes of healthy and bovine leukemia virus-infected cows].

A modified cytochemical technique was employed to investigate the unspecific acid naphthylacetate esterase of lymphocytes in the peripheral blood of a total of 64 clinically normal cows, having negative serologic and hematologic reaction for leukosis and of 42 cows with severalfold, well-manifested positive serologic response for the presence of the bovine leukosis virus with no changes in the hematologic data. With the normal animals an average of 6.86 per cent of the peripheral lymphocytes were found to be active--in the form of large dark-brown to dark red-brown granules--and such cells were considered to be T mu mature lymphocytes, while in 53.

[Modified method for the rapid determination of the magnesium requirement of cows and sheep].

Korzeniowski's method was modified in preliminary investigations on the semiquantitative determination of magnesium in urine as follows: solution A--citric acid, 6.5 g; 8-oxicholine, 1.8-2.

[Therapeutic and prophylactic action of monensin and nicotinic acid in cows with subclinical ketosis].

Experiments were carried out with sheep and cows on the basis of a limited feed ration--the giving of concentrates was discontinued, and the ration of alfalfa hay was replaced with meadow hay and a monensine supplement (at the rate of 20-25 mg for sheep and 250 mg for cows, daily), and nicotinic acid (at 0.8 g and 6.0 g, respectively).

[Clinico-experimental and clinical studies of a ketomethine preparation in sheep and cows].

Some of the clinical parameters of the preparation ketomethyne, containing propylene glycol, methyonine, and Co bichloride, were comparatively studied with the analogous preparation ursoketin at oral application to sheep and cows. It was found that ketomethyne raised more slowly the level of blood sugar than ursoketin, but maintained it by 24-35 per cent higher than the initial level from the 6th to the 24th hour following treatment. The oral application of ketomethyne had no unfavourable effect on the rumen activity, the general clinical indices body temperature, pulse, respiration), the hematologic indices as hemoglobin and erythrocyte and leukocyte count, and the appetite.

[Changes in the serum enzymes and clinical and clinico-biochemical indices of cows with subclinical ketosis].

Investigations were carried out with a total of 276 high-producing and clinically healthy cows that had freshly calved on 11 farms, being divided into groups according to the extent to which ketonuria was present if al all. Whole blood and blood serum were sampled to determine the ketone bodies, blood sugar, erythrocyte and leukocyte counts, hemoglobin, inorganic phosphorus, Ca, Mg, total protein, carotene, and activity of the GOT and GPT enzymes as well as the activity of lactic acid dehydrogenase, alkaline phosphatase, aldolase, and leucine aminopeptidase. Studied were the body temperature, the pulse rate, and the respiration rate.

[5'-nucleotidase activity in the organs of cattle, sheep and swine].

Studied was the activity of 5'-nucleotidase (EC3.1.3.

[Bovicystan treatment of subclinical ketosis in cows].

Experiments with normal animals (10 sheep and 15 cows) revealed that the intravenous injection of bovicystan led to a considerable and dependable rise of the blood sugar for 2 to 36 hours the peak being at the 6th hour. The treatment of 95 freshly calved cows on 8 farms with signs of subclinical ketosis (ketonemia 22.4 +/- 1.

[Hyperglycemic and therapeutic action of nontranquilizing doses of xylazine in healthy sheep and cows and in cows with subclinical ketosis].

Experiments with 19 sheep and 26 cows revealed that subcutaneous and muscular injection of xylasine (Rompun - Bayer at 0.04 to 0.08 mg/kg body mass) led to a dependable increase in the level of blood sugar by 50-220 per cent for a period of 1 to 3 hrs.

[Leucine and alanine aminopeptidase activity in the organs of cattle, sheep and swine].

Studied was the activity of leucine-aminopeptidase and alanine-aminopeptidase in fresh tissue homogenates of liver, spleen, kidney, heart, pancreas, femoral muscle, stomach (rumen), small intestine, and lung taken from 8 cattle, sheep, and pigs. Both enzymes showed ubiquity. Leucine-aminopeptidase exhibited highest activity in the spleen of pigs and the kidney of sheep and cattle.

[Thermal sensitivity of aldolase isoenzymes in the organs of cattle, sheep and swine].

During the investigation of homogenates from organs of cattle, sheep and swine (a heart, a kidney, a pancreas, a liver, a thigh muscle, a spleen, a lung) the highest activity of aldolase was proved in the bodily muscles, whereas in the other organs it did not show great differences. Thus it is determined as an ubiquitous enzyme in all these kinds of farm animals. After heating at 61 degrees C for 15 min.

[Alkaline phosphatase activity and properties of the blood serum and organs of hens].

Investigations were carried out with homogenates from the organs of hens (liver, duodenum, pancreas, spleen, kidney, glandular and muscular stomach, ovary resp. testicle, periosteum,) as well as serums in which the general activity of the alkaline phosphatase was determined and parallel to this after an inhibition in the presence of 10 mM 1-phenylalanine or different concentrations (0.25--4 M) or urea, as well as with values of pH ranging from 9.

[Enzymatic activity study of the organs and blood serum of geese].

Studies on organ homogenates of 22 one-year-old healthy geese indicated ubiquiternal distribution of GOT and GPT transaminases, lactate dehydrogenase, alkaline and acid phosphatase, aldolase and kreatine phosphokinase, without the presence of any pronounced organ specificity of some of the named enzymes. It is presumed that the investigation on these enzymes in goose blood serum can be of use only for determining the degree and the course of a given disease, but not for organ-localization of the disease's process.

[Comparative study of the muramidase (lysozyme) activity in the blood cells of laboratory and agricultural animals].

Comparative studies have been carried out on the blood of certain laboratory and farm animals and birds, whereby the activity of muramidase (lysozyme) is established in neutrophilic cells of mice, guinea pigs, rats, rabbits, dogs, hens, turkeys and geese and in the monocytes of rabbits and dogs. The percentage of cells with muramidase activity manifests species features. No cells with a presence of muramidase activity have been found in the perypheral blood of cattle, sheep, pigs, goats, horses and bullalos.

[Biochemical study of serum from sheep infected with Brucella ovis].

Blood serum was used of 49 rams and 20 ewew infected with Brucell ovis. The study on the total protein and the protein fractions of rams revealed that there existed gamma-globulinemia, the percent of the gamma-globulin rise showing a positive correlation with the morphologic changes in the testes. The alfa-globulins were found to rise immediately following the experimental infecting of the sheep for about a month, after which they came back to normal.

[Biochemical and histological studies of cows with ketosis].

Biochemical and histological investigations were carried out with cows affected with ketosis in a subclinical and clinical form. Parellel studies were performed with normal (control) cows originating from the herds with the ill animals and manifesting the same physiologic status. It was found that the blood level of glucose in the diseased cows was lowered more than 2.

[Comparative electrophoretic study of the molecular forms of alkaline phosphatase in the leukocytes of agricultural animals].

The alkaline phosphatase enzyme, isolated by Morton's method from leukocytes of sheep, goats, and pigs gave after agarose elctrophoresis two isoenzyme fractions moving to the positive pole at the sites of the alpha 1- and alpha 2-globulins of the blood serum. In bovine leukocytes, besides these two fractions there was a third one that moved more slowly in the zone of the beta-globulins. In horses the alkaline phosphatase of leukocytes produced a wide band within the zones of the beta-globulins and the albumins.

[Study of disorders in calcium and phosphorus metabolism in the intensive fattening of young cattle].

Studied were mass disease outbreaks on eight farms in calves intensively fed concentrate mixtures that were rich in phosphorus compounds. Clinically, there were selling and deformations of the joints with pains. The tarsal and carpal joints were chiefly involved.