Chitooligosaccharides (COS) generated from either chitin (chitin oligosaccharides) or chitosan (chitosan oligosaccharides) have a wide range of applications in agriculture, medicine, and other fields. Here, we report the characterization of a chitosanase from (Csn) and the importance of a tryptophan (Trp), W204, for Csn activity. Csn hydrolyzed the chitosan polymer by an endo mode.
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